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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-109627794-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=109627794&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 109627794,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000528667.7",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "NM_001368809.2",
          "protein_id": "NP_001355738.1",
          "transcript_support_level": null,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 825,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2478,
          "cdna_start": 1675,
          "cdna_end": null,
          "cdna_length": 4081,
          "mane_select": "ENST00000528667.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "ENST00000528667.7",
          "protein_id": "ENSP00000436541.2",
          "transcript_support_level": 1,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 825,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2478,
          "cdna_start": 1675,
          "cdna_end": null,
          "cdna_length": 4081,
          "mane_select": "NM_001368809.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.890G>C",
          "hgvs_p": "p.Arg297Pro",
          "transcript": "ENST00000342115.8",
          "protein_id": "ENSP00000345498.4",
          "transcript_support_level": 1,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": 1322,
          "cdna_end": null,
          "cdna_length": 3728,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.1034G>C",
          "hgvs_p": null,
          "transcript": "ENST00000526301.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3065,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "ENST00000369840.7",
          "protein_id": "ENSP00000358855.3",
          "transcript_support_level": 5,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 836,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2511,
          "cdna_start": 1044,
          "cdna_end": null,
          "cdna_length": 3130,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "NM_004037.9",
          "protein_id": "NP_004028.4",
          "transcript_support_level": null,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 825,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2478,
          "cdna_start": 1005,
          "cdna_end": null,
          "cdna_length": 3411,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "ENST00000256578.8",
          "protein_id": "ENSP00000256578.4",
          "transcript_support_level": 5,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 825,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2478,
          "cdna_start": 1493,
          "cdna_end": null,
          "cdna_length": 3899,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.908G>C",
          "hgvs_p": "p.Arg303Pro",
          "transcript": "NM_001308170.1",
          "protein_id": "NP_001295099.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 804,
          "cds_start": 908,
          "cds_end": null,
          "cds_length": 2415,
          "cdna_start": 1102,
          "cdna_end": null,
          "cdna_length": 3512,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.890G>C",
          "hgvs_p": "p.Arg297Pro",
          "transcript": "NM_139156.4",
          "protein_id": "NP_631895.1",
          "transcript_support_level": null,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": 1322,
          "cdna_end": null,
          "cdna_length": 3728,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.890G>C",
          "hgvs_p": "p.Arg297Pro",
          "transcript": "ENST00000531734.6",
          "protein_id": "ENSP00000433739.2",
          "transcript_support_level": 4,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 890,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": 1151,
          "cdna_end": null,
          "cdna_length": 2658,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro",
          "transcript": "ENST00000358729.9",
          "protein_id": "ENSP00000351573.5",
          "transcript_support_level": 2,
          "aa_start": 324,
          "aa_end": null,
          "aa_length": 788,
          "cds_start": 971,
          "cds_end": null,
          "cds_length": 2367,
          "cdna_start": 971,
          "cdna_end": null,
          "cdna_length": 2783,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.779G>C",
          "hgvs_p": "p.Arg260Pro",
          "transcript": "NM_001257361.2",
          "protein_id": "NP_001244290.1",
          "transcript_support_level": null,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 761,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 2286,
          "cdna_start": 941,
          "cdna_end": null,
          "cdna_length": 3347,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.779G>C",
          "hgvs_p": "p.Arg260Pro",
          "transcript": "ENST00000528454.5",
          "protein_id": "ENSP00000437164.1",
          "transcript_support_level": 2,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 761,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 2286,
          "cdna_start": 1156,
          "cdna_end": null,
          "cdna_length": 2728,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.779G>C",
          "hgvs_p": "p.Arg260Pro",
          "transcript": "ENST00000531203.6",
          "protein_id": "ENSP00000431975.2",
          "transcript_support_level": 4,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 761,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 2286,
          "cdna_start": 1093,
          "cdna_end": null,
          "cdna_length": 2600,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.653G>C",
          "hgvs_p": "p.Arg218Pro",
          "transcript": "ENST00000476688.3",
          "protein_id": "ENSP00000437025.2",
          "transcript_support_level": 3,
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          "aa_end": null,
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          "cds_length": 2160,
          "cdna_start": 653,
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          "cdna_length": 3052,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "c.371G>C",
          "hgvs_p": "p.Arg124Pro",
          "transcript": "ENST00000667949.2",
          "protein_id": "ENSP00000499465.2",
          "transcript_support_level": null,
          "aa_start": 124,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 371,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": 1191,
          "cdna_end": null,
          "cdna_length": 3590,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.1590G>C",
          "hgvs_p": null,
          "transcript": "ENST00000474459.6",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3989,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.1694G>C",
          "hgvs_p": null,
          "transcript": "ENST00000486282.7",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4600,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.1305G>C",
          "hgvs_p": null,
          "transcript": "ENST00000524975.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 4145,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.1487G>C",
          "hgvs_p": null,
          "transcript": "ENST00000525415.2",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3878,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AMPD2",
          "gene_hgnc_id": 469,
          "hgvs_c": "n.826G>C",
          "hgvs_p": null,
          "transcript": "ENST00000527846.7",
          "protein_id": null,
          "transcript_support_level": 4,
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      ],
      "gene_symbol": "AMPD2",
      "gene_hgnc_id": 469,
      "dbsnp": "rs746332433",
      "frequency_reference_population": 6.84075e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84075e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9749153852462769,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.23999999463558197,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.956,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9993,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.47,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.884,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.24,
      "spliceai_max_prediction": "Uncertain_significance",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": 10,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PM5,PP3_Strong,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 10,
          "benign_score": 0,
          "pathogenic_score": 10,
          "criteria": [
            "PM2",
            "PM5",
            "PP3_Strong",
            "PP5_Moderate"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000528667.7",
          "gene_symbol": "AMPD2",
          "hgnc_id": 469,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.971G>C",
          "hgvs_p": "p.Arg324Pro"
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      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}