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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-225838705-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=225838705&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 225838705,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000120.4",
      "consequences": [
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "NM_001136018.4",
          "protein_id": "NP_001129490.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000272167.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001136018.4"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000272167.10",
          "protein_id": "ENSP00000272167.5",
          "transcript_support_level": 1,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001136018.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000272167.10"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000366837.5",
          "protein_id": "ENSP00000355802.4",
          "transcript_support_level": 1,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000366837.5"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000614058.4",
          "protein_id": "ENSP00000480004.1",
          "transcript_support_level": 1,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000614058.4"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869511.1",
          "protein_id": "ENSP00000539570.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1479,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869511.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869476.1",
          "protein_id": "ENSP00000539535.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 488,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869476.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869509.1",
          "protein_id": "ENSP00000539568.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 488,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1467,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869509.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869484.1",
          "protein_id": "ENSP00000539543.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869484.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869483.1",
          "protein_id": "ENSP00000539542.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869483.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.452A>T",
          "hgvs_p": "p.His151Leu",
          "transcript": "ENST00000869517.1",
          "protein_id": "ENSP00000539576.1",
          "transcript_support_level": null,
          "aa_start": 151,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 452,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000869517.1"
        },
        {
          "aa_ref": "H",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869470.1",
          "protein_id": "ENSP00000539529.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000869470.1"
        },
        {
          "aa_ref": "H",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 4,
          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869516.1",
          "protein_id": "ENSP00000539575.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1398,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "H",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869519.1",
          "protein_id": "ENSP00000539578.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 465,
          "cds_start": 416,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "H",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "NM_000120.4",
          "protein_id": "NP_000111.1",
          "transcript_support_level": null,
          "aa_start": 139,
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          "cds_start": 416,
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        {
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          ],
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          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "NM_001291163.2",
          "protein_id": "NP_001278092.1",
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          "aa_end": null,
          "aa_length": 455,
          "cds_start": 416,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001291163.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "NM_001378426.1",
          "protein_id": "NP_001365355.1",
          "transcript_support_level": null,
          "aa_start": 139,
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          "cds_start": 416,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "H",
          "aa_alt": "L",
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 4,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "NM_001378427.1",
          "protein_id": "NP_001365356.1",
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        },
        {
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          ],
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          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "EPHX1",
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          "hgvs_c": "c.416A>T",
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          "transcript": "ENST00000869469.1",
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        },
        {
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          ],
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          "gene_symbol": "EPHX1",
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          "hgvs_c": "c.416A>T",
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          "protein_id": "ENSP00000539530.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000869471.1"
        },
        {
          "aa_ref": "H",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu",
          "transcript": "ENST00000869473.1",
          "protein_id": "ENSP00000539532.1",
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          "cds_start": null,
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        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
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          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "EPHX1",
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          "hgvs_c": "n.612A>T",
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          "transcript": "NR_165627.1",
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          "cdna_start": null,
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          "biotype": "pseudogene",
          "feature": "NR_165627.1"
        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 6,
          "intron_rank": 3,
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          "gene_symbol": "EPHX1",
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          "hgvs_c": "n.370-3709A>T",
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          "transcript": "NR_165624.1",
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          "transcript_support_level": null,
          "aa_start": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "pseudogene",
          "feature": "NR_165624.1"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "EPHX1",
          "gene_hgnc_id": 3401,
          "hgvs_c": "c.*8A>T",
          "hgvs_p": null,
          "transcript": "ENST00000448202.5",
          "protein_id": "ENSP00000408469.1",
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": 135,
          "cds_start": null,
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          "cds_length": 408,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000448202.5"
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      ],
      "gene_symbol": "EPHX1",
      "gene_hgnc_id": 3401,
      "dbsnp": "rs2234922",
      "frequency_reference_population": 0.000009294556,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 15,
      "gnomad_exomes_af": 0.00000957668,
      "gnomad_genomes_af": 0.00000658051,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.045999377965927124,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.016,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1031,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.59,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.84,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000120.4",
          "gene_symbol": "EPHX1",
          "hgnc_id": 3401,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.416A>T",
          "hgvs_p": "p.His139Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}