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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-236749157-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=236749157&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 236749157,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001103.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "NM_001103.4",
          "protein_id": "NP_001094.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 894,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2685,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000366578.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001103.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000366578.6",
          "protein_id": "ENSP00000355537.4",
          "transcript_support_level": 1,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 894,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2685,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001103.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000366578.6"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000542672.7",
          "protein_id": "ENSP00000443495.1",
          "transcript_support_level": 1,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 894,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2685,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000542672.7"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1660C>T",
          "hgvs_p": "p.Leu554Phe",
          "transcript": "ENST00000879537.1",
          "protein_id": "ENSP00000549596.1",
          "transcript_support_level": null,
          "aa_start": 554,
          "aa_end": null,
          "aa_length": 931,
          "cds_start": 1660,
          "cds_end": null,
          "cds_length": 2796,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879537.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1660C>T",
          "hgvs_p": "p.Leu554Phe",
          "transcript": "ENST00000967370.1",
          "protein_id": "ENSP00000637429.1",
          "transcript_support_level": null,
          "aa_start": 554,
          "aa_end": null,
          "aa_length": 931,
          "cds_start": 1660,
          "cds_end": null,
          "cds_length": 2796,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967370.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1651C>T",
          "hgvs_p": "p.Leu551Phe",
          "transcript": "ENST00000967362.1",
          "protein_id": "ENSP00000637421.1",
          "transcript_support_level": null,
          "aa_start": 551,
          "aa_end": null,
          "aa_length": 928,
          "cds_start": 1651,
          "cds_end": null,
          "cds_length": 2787,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967362.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1639C>T",
          "hgvs_p": "p.Leu547Phe",
          "transcript": "ENST00000879533.1",
          "protein_id": "ENSP00000549592.1",
          "transcript_support_level": null,
          "aa_start": 547,
          "aa_end": null,
          "aa_length": 924,
          "cds_start": 1639,
          "cds_end": null,
          "cds_length": 2775,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879533.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000967365.1",
          "protein_id": "ENSP00000637424.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 909,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2730,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967365.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000879532.1",
          "protein_id": "ENSP00000549591.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 907,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2724,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879532.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1582C>T",
          "hgvs_p": "p.Leu528Phe",
          "transcript": "ENST00000967363.1",
          "protein_id": "ENSP00000637422.1",
          "transcript_support_level": null,
          "aa_start": 528,
          "aa_end": null,
          "aa_length": 905,
          "cds_start": 1582,
          "cds_end": null,
          "cds_length": 2718,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967363.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1561C>T",
          "hgvs_p": "p.Leu521Phe",
          "transcript": "ENST00000967352.1",
          "protein_id": "ENSP00000637411.1",
          "transcript_support_level": null,
          "aa_start": 521,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 1561,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967352.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1552C>T",
          "hgvs_p": "p.Leu518Phe",
          "transcript": "ENST00000879534.1",
          "protein_id": "ENSP00000549593.1",
          "transcript_support_level": null,
          "aa_start": 518,
          "aa_end": null,
          "aa_length": 895,
          "cds_start": 1552,
          "cds_end": null,
          "cds_length": 2688,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879534.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "NM_001278343.2",
          "protein_id": "NP_001265272.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 894,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2685,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001278343.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000879538.1",
          "protein_id": "ENSP00000549597.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 893,
          "cds_start": 1549,
          "cds_end": null,
          "cds_length": 2682,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000879538.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1543C>T",
          "hgvs_p": "p.Leu515Phe",
          "transcript": "ENST00000967348.1",
          "protein_id": "ENSP00000637407.1",
          "transcript_support_level": null,
          "aa_start": 515,
          "aa_end": null,
          "aa_length": 892,
          "cds_start": 1543,
          "cds_end": null,
          "cds_length": 2679,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967348.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe",
          "transcript": "ENST00000967355.1",
          "protein_id": "ENSP00000637414.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 892,
          "cds_start": 1549,
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          "cds_length": 2679,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967355.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1534C>T",
          "hgvs_p": "p.Leu512Phe",
          "transcript": "ENST00000967354.1",
          "protein_id": "ENSP00000637413.1",
          "transcript_support_level": null,
          "aa_start": 512,
          "aa_end": null,
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          "cds_start": 1534,
          "cds_end": null,
          "cds_length": 2670,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000967354.1"
        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1531C>T",
          "hgvs_p": "p.Leu511Phe",
          "transcript": "ENST00000967356.1",
          "protein_id": "ENSP00000637415.1",
          "transcript_support_level": null,
          "aa_start": 511,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 1531,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000967356.1"
        },
        {
          "aa_ref": "L",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1528C>T",
          "hgvs_p": "p.Leu510Phe",
          "transcript": "ENST00000879535.1",
          "protein_id": "ENSP00000549594.1",
          "transcript_support_level": null,
          "aa_start": 510,
          "aa_end": null,
          "aa_length": 887,
          "cds_start": 1528,
          "cds_end": null,
          "cds_length": 2664,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879535.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACTN2",
          "gene_hgnc_id": 164,
          "hgvs_c": "c.1519C>T",
          "hgvs_p": "p.Leu507Phe",
          "transcript": "ENST00000967357.1",
          "protein_id": "ENSP00000637416.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 884,
          "cds_start": 1519,
          "cds_end": null,
          "cds_length": 2655,
          "cdna_start": null,
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      "gene_symbol": "ACTN2",
      "gene_hgnc_id": 164,
      "dbsnp": "rs759778745",
      "frequency_reference_population": 0.000017967113,
      "hom_count_reference_population": 0,
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      "gnomad_exomes_af": 0.0000184694,
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      "gnomad_exomes_ac": 27,
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      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8594377040863037,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.605,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.5567,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 0.709,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3_Moderate,BP6,BS2",
      "acmg_by_gene": [
        {
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          "benign_score": 5,
          "pathogenic_score": 2,
          "criteria": [
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            "BS2"
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          "verdict": "Likely_benign",
          "transcript": "NM_001103.4",
          "gene_symbol": "ACTN2",
          "hgnc_id": 164,
          "effects": [
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          "inheritance_mode": "AD,Unknown",
          "hgvs_c": "c.1549C>T",
          "hgvs_p": "p.Leu517Phe"
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      ],
      "clinvar_disease": "Cardiomyopathy,Cardiovascular phenotype,Dilated cardiomyopathy 1AA,Primary familial hypertrophic cardiomyopathy",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:1",
      "phenotype_combined": "Primary familial hypertrophic cardiomyopathy;Dilated cardiomyopathy 1AA|Cardiomyopathy|Cardiovascular phenotype",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}