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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-39844131-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=39844131&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 39844131,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000316891.10",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.1204C>T",
          "hgvs_p": "p.Arg402*",
          "transcript": "NM_017646.6",
          "protein_id": "NP_060116.2",
          "transcript_support_level": null,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 1204,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": 1217,
          "cdna_end": null,
          "cdna_length": 5051,
          "mane_select": "ENST00000316891.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.1204C>T",
          "hgvs_p": "p.Arg402*",
          "transcript": "ENST00000316891.10",
          "protein_id": "ENSP00000321810.5",
          "transcript_support_level": 1,
          "aa_start": 402,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 1204,
          "cds_end": null,
          "cds_length": 1404,
          "cdna_start": 1217,
          "cdna_end": null,
          "cdna_length": 5051,
          "mane_select": "NM_017646.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.1126C>T",
          "hgvs_p": "p.Arg376*",
          "transcript": "ENST00000372818.5",
          "protein_id": "ENSP00000361905.1",
          "transcript_support_level": 1,
          "aa_start": 376,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 1126,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": 1141,
          "cdna_end": null,
          "cdna_length": 2034,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.958C>T",
          "hgvs_p": "p.Arg320*",
          "transcript": "ENST00000441669.6",
          "protein_id": "ENSP00000388333.2",
          "transcript_support_level": 1,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": 958,
          "cdna_end": null,
          "cdna_length": 1851,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.58C>T",
          "hgvs_p": "p.Arg20*",
          "transcript": "ENST00000541099.5",
          "protein_id": "ENSP00000437896.1",
          "transcript_support_level": 1,
          "aa_start": 20,
          "aa_end": null,
          "aa_length": 85,
          "cds_start": 58,
          "cds_end": null,
          "cds_length": 258,
          "cdna_start": 315,
          "cdna_end": null,
          "cdna_length": 545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.*44C>T",
          "hgvs_p": null,
          "transcript": "ENST00000462797.5",
          "protein_id": "ENSP00000473773.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.*626C>T",
          "hgvs_p": null,
          "transcript": "ENST00000495175.6",
          "protein_id": "ENSP00000474264.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1156,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.*44C>T",
          "hgvs_p": null,
          "transcript": "ENST00000462797.5",
          "protein_id": "ENSP00000473773.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.*626C>T",
          "hgvs_p": null,
          "transcript": "ENST00000495175.6",
          "protein_id": "ENSP00000474264.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1156,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.1126C>T",
          "hgvs_p": "p.Arg376*",
          "transcript": "NM_001312691.1",
          "protein_id": "NP_001299620.1",
          "transcript_support_level": null,
          "aa_start": 376,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 1126,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": 1146,
          "cdna_end": null,
          "cdna_length": 2059,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.958C>T",
          "hgvs_p": "p.Arg320*",
          "transcript": "NM_001312692.1",
          "protein_id": "NP_001299621.1",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": 978,
          "cdna_end": null,
          "cdna_length": 1891,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.292C>T",
          "hgvs_p": "p.Arg98*",
          "transcript": "ENST00000537440.5",
          "protein_id": "ENSP00000437700.1",
          "transcript_support_level": 5,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 163,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 492,
          "cdna_start": 1104,
          "cdna_end": null,
          "cdna_length": 2026,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "c.781C>T",
          "hgvs_p": "p.Arg261*",
          "transcript": "XM_047423225.1",
          "protein_id": "XP_047279181.1",
          "transcript_support_level": null,
          "aa_start": 261,
          "aa_end": null,
          "aa_length": 326,
          "cds_start": 781,
          "cds_end": null,
          "cds_length": 981,
          "cdna_start": 934,
          "cdna_end": null,
          "cdna_length": 4768,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.388C>T",
          "hgvs_p": null,
          "transcript": "ENST00000465417.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 499,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.439C>T",
          "hgvs_p": null,
          "transcript": "ENST00000491865.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 738,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.*1877C>T",
          "hgvs_p": null,
          "transcript": "ENST00000648678.1",
          "protein_id": "ENSP00000497805.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5930,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.1220C>T",
          "hgvs_p": null,
          "transcript": "NR_132401.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2133,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.1078C>T",
          "hgvs_p": null,
          "transcript": "NR_132402.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 1991,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.1074C>T",
          "hgvs_p": null,
          "transcript": "NR_132403.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 1987,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.1074C>T",
          "hgvs_p": null,
          "transcript": "NR_132404.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1987,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIT1",
          "gene_hgnc_id": 20286,
          "hgvs_c": "n.1070C>T",
          "hgvs_p": null,
          "transcript": "NR_132405.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
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      "gene_symbol": "TRIT1",
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      "dbsnp": "rs367752391",
      "frequency_reference_population": 0.000006815754,
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      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000547317,
      "gnomad_genomes_af": 0.0000197068,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6299999952316284,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.63,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 3.829,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PVS1_Strong,PM2,PP5",
      "acmg_by_gene": [
        {
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          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
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            "PM2",
            "PP5"
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          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000316891.10",
          "gene_symbol": "TRIT1",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.1204C>T",
          "hgvs_p": "p.Arg402*"
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      ],
      "clinvar_disease": "Combined oxidative phosphorylation deficiency 35,TRIT1 Deficiency,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:1 US:2",
      "phenotype_combined": "TRIT1 Deficiency|Combined oxidative phosphorylation deficiency 35|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}