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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 1-97082391-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=1&pos=97082391&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "1",
      "pos": 97082391,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000370192.8",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2846A>T",
          "hgvs_p": "p.Asp949Val",
          "transcript": "NM_000110.4",
          "protein_id": "NP_000101.2",
          "transcript_support_level": null,
          "aa_start": 949,
          "aa_end": null,
          "aa_length": 1025,
          "cds_start": 2846,
          "cds_end": null,
          "cds_length": 3078,
          "cdna_start": 2958,
          "cdna_end": null,
          "cdna_length": 4423,
          "mane_select": "ENST00000370192.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2846A>T",
          "hgvs_p": "p.Asp949Val",
          "transcript": "ENST00000370192.8",
          "protein_id": "ENSP00000359211.3",
          "transcript_support_level": 1,
          "aa_start": 949,
          "aa_end": null,
          "aa_length": 1025,
          "cds_start": 2846,
          "cds_end": null,
          "cds_length": 3078,
          "cdna_start": 2958,
          "cdna_end": null,
          "cdna_length": 4423,
          "mane_select": "NM_000110.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2735A>T",
          "hgvs_p": "p.Asp912Val",
          "transcript": "XM_017000507.2",
          "protein_id": "XP_016855996.1",
          "transcript_support_level": null,
          "aa_start": 912,
          "aa_end": null,
          "aa_length": 988,
          "cds_start": 2735,
          "cds_end": null,
          "cds_length": 2967,
          "cdna_start": 2872,
          "cdna_end": null,
          "cdna_length": 4337,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2630A>T",
          "hgvs_p": "p.Asp877Val",
          "transcript": "XM_005270562.3",
          "protein_id": "XP_005270619.2",
          "transcript_support_level": null,
          "aa_start": 877,
          "aa_end": null,
          "aa_length": 953,
          "cds_start": 2630,
          "cds_end": null,
          "cds_length": 2862,
          "cdna_start": 2767,
          "cdna_end": null,
          "cdna_length": 4232,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2618A>T",
          "hgvs_p": "p.Asp873Val",
          "transcript": "XM_047448076.1",
          "protein_id": "XP_047304032.1",
          "transcript_support_level": null,
          "aa_start": 873,
          "aa_end": null,
          "aa_length": 949,
          "cds_start": 2618,
          "cds_end": null,
          "cds_length": 2850,
          "cdna_start": 2876,
          "cdna_end": null,
          "cdna_length": 4341,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "D",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DPYD",
          "gene_hgnc_id": 3012,
          "hgvs_c": "c.2519A>T",
          "hgvs_p": "p.Asp840Val",
          "transcript": "XM_047448077.1",
          "protein_id": "XP_047304033.1",
          "transcript_support_level": null,
          "aa_start": 840,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2519,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": 2656,
          "cdna_end": null,
          "cdna_length": 4121,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "DPYD",
      "gene_hgnc_id": 3012,
      "dbsnp": "rs67376798",
      "frequency_reference_population": 0.0050438135,
      "hom_count_reference_population": 32,
      "allele_count_reference_population": 8139,
      "gnomad_exomes_af": 0.00522778,
      "gnomad_genomes_af": 0.00327781,
      "gnomad_exomes_ac": 7640,
      "gnomad_genomes_ac": 499,
      "gnomad_exomes_homalt": 29,
      "gnomad_genomes_homalt": 3,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.13372507691383362,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.962,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.2925,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": 0.46,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.819,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3,BP4_Moderate,BS1_Supporting,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 7,
          "pathogenic_score": 1,
          "criteria": [
            "PP3",
            "BP4_Moderate",
            "BS1_Supporting",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000370192.8",
          "gene_symbol": "DPYD",
          "hgnc_id": 3012,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2846A>T",
          "hgvs_p": "p.Asp949Val"
        }
      ],
      "clinvar_disease": "DPYD-related disorder,Dihydropyrimidine dehydrogenase deficiency,Fluorouracil response,Inborn genetic diseases,capecitabine response - Toxicity,fluorouracil response - Other,fluorouracil response - Toxicity,not provided,tegafur response - Toxicity",
      "clinvar_classification": "drug response",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "P:6 LP:1 US:1 LB:1 B:1 O:5",
      "phenotype_combined": "Dihydropyrimidine dehydrogenase deficiency|fluorouracil response - Toxicity|fluorouracil response - Other|capecitabine response - Toxicity|DPYD-related disorder|not provided|Fluorouracil response|Inborn genetic diseases|tegafur response - Toxicity",
      "pathogenicity_classification_combined": "drug response",
      "custom_annotations": null
    }
  ],
  "message": null
}