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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 10-3067617-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=10&pos=3067617&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "10",
      "pos": 3067617,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001410880.1",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "NM_002627.5",
          "protein_id": "NP_002618.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000381125.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002627.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000381125.9",
          "protein_id": "ENSP00000370517.4",
          "transcript_support_level": 1,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002627.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381125.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "NM_001410880.1",
          "protein_id": "NP_001397809.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410880.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000699222.1",
          "protein_id": "ENSP00000514216.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000699222.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963518.1",
          "protein_id": "ENSP00000633577.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 827,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2484,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963518.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963522.1",
          "protein_id": "ENSP00000633581.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 817,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2454,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963522.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963523.1",
          "protein_id": "ENSP00000633582.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 816,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2451,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963523.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963519.1",
          "protein_id": "ENSP00000633578.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 815,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2448,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963519.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963520.1",
          "protein_id": "ENSP00000633579.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963520.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867378.1",
          "protein_id": "ENSP00000537437.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 784,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2355,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867378.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867383.1",
          "protein_id": "ENSP00000537442.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867383.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867380.1",
          "protein_id": "ENSP00000537439.1",
          "transcript_support_level": null,
          "aa_start": 8,
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          "aa_length": 782,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2349,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867380.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867379.1",
          "protein_id": "ENSP00000537438.1",
          "transcript_support_level": null,
          "aa_start": 8,
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          "aa_length": 769,
          "cds_start": 22,
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          "cds_length": 2310,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867379.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "NM_001323068.2",
          "protein_id": "NP_001309997.1",
          "transcript_support_level": null,
          "aa_start": 8,
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          "aa_length": 733,
          "cds_start": 22,
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          "cds_length": 2202,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
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          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867382.1",
          "protein_id": "ENSP00000537441.1",
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          "aa_start": 8,
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          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2202,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867382.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000867381.1",
          "protein_id": "ENSP00000537440.1",
          "transcript_support_level": null,
          "aa_start": 8,
          "aa_end": null,
          "aa_length": 719,
          "cds_start": 22,
          "cds_end": null,
          "cds_length": 2160,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000867381.1"
        },
        {
          "aa_ref": "A",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "ENST00000963521.1",
          "protein_id": "ENSP00000633580.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 509,
          "cds_start": 22,
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          "mane_select": null,
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        },
        {
          "aa_ref": "A",
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          "canonical": false,
          "protein_coding": true,
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          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro",
          "transcript": "XM_005252466.5",
          "protein_id": "XP_005252523.1",
          "transcript_support_level": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_005252466.5"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
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          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.-209G>C",
          "hgvs_p": null,
          "transcript": "NM_001323071.2",
          "protein_id": "NP_001310000.1",
          "transcript_support_level": null,
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          "aa_length": 746,
          "cds_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001323071.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "c.-481G>C",
          "hgvs_p": null,
          "transcript": "NM_001323069.2",
          "protein_id": "NP_001309998.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 615,
          "cds_start": null,
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          "cds_length": 1848,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001323069.2"
        },
        {
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        {
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          "protein_coding": false,
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          ],
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          "gene_symbol": "PFKP-DT",
          "gene_hgnc_id": 55177,
          "hgvs_c": "n.98+1869C>G",
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          "transcript": "ENST00000820070.1",
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          "biotype": "pseudogene",
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        },
        {
          "aa_ref": null,
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
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          "exon_count": 2,
          "intron_rank": null,
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          "gene_symbol": "PFKP",
          "gene_hgnc_id": 8878,
          "hgvs_c": "n.-55G>C",
          "hgvs_p": null,
          "transcript": "ENST00000495715.1",
          "protein_id": null,
          "transcript_support_level": 3,
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          "aa_length": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000495715.1"
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      ],
      "gene_symbol": "PFKP",
      "gene_hgnc_id": 8878,
      "dbsnp": "rs984216789",
      "frequency_reference_population": 0.00010394888,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 159,
      "gnomad_exomes_af": 0.000110352,
      "gnomad_genomes_af": 0.0000459957,
      "gnomad_exomes_ac": 152,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0925009548664093,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.161,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0666,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.38,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.058,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001410880.1",
          "gene_symbol": "PFKP",
          "hgnc_id": 8878,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.22G>C",
          "hgvs_p": "p.Ala8Pro"
        },
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000820060.1",
          "gene_symbol": "PFKP-DT",
          "hgnc_id": 55177,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.292+304C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}