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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-22250758-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=22250758&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 22250758,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_213599.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.1031C>G",
          "hgvs_p": "p.Pro344Arg",
          "transcript": "NM_213599.3",
          "protein_id": "NP_998764.1",
          "transcript_support_level": null,
          "aa_start": 344,
          "aa_end": null,
          "aa_length": 913,
          "cds_start": 1031,
          "cds_end": null,
          "cds_length": 2742,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000324559.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_213599.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.1031C>G",
          "hgvs_p": "p.Pro344Arg",
          "transcript": "ENST00000324559.9",
          "protein_id": "ENSP00000315371.9",
          "transcript_support_level": 1,
          "aa_start": 344,
          "aa_end": null,
          "aa_length": 913,
          "cds_start": 1031,
          "cds_end": null,
          "cds_length": 2742,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_213599.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000324559.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.1028C>G",
          "hgvs_p": "p.Pro343Arg",
          "transcript": "NM_001142649.2",
          "protein_id": "NP_001136121.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 912,
          "cds_start": 1028,
          "cds_end": null,
          "cds_length": 2739,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001142649.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.989C>G",
          "hgvs_p": "p.Pro330Arg",
          "transcript": "NM_001410963.1",
          "protein_id": "NP_001397892.1",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 899,
          "cds_start": 989,
          "cds_end": null,
          "cds_length": 2700,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410963.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.989C>G",
          "hgvs_p": "p.Pro330Arg",
          "transcript": "ENST00000682341.1",
          "protein_id": "ENSP00000508251.1",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 899,
          "cds_start": 989,
          "cds_end": null,
          "cds_length": 2700,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000682341.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.986C>G",
          "hgvs_p": "p.Pro329Arg",
          "transcript": "NM_001410964.1",
          "protein_id": "NP_001397893.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410964.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.986C>G",
          "hgvs_p": "p.Pro329Arg",
          "transcript": "ENST00000684663.1",
          "protein_id": "ENSP00000508009.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 898,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 2697,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000684663.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.953C>G",
          "hgvs_p": "p.Pro318Arg",
          "transcript": "NM_001441294.1",
          "protein_id": "NP_001428223.1",
          "transcript_support_level": null,
          "aa_start": 318,
          "aa_end": null,
          "aa_length": 887,
          "cds_start": 953,
          "cds_end": null,
          "cds_length": 2664,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441294.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.950C>G",
          "hgvs_p": "p.Pro317Arg",
          "transcript": "NM_001441295.1",
          "protein_id": "NP_001428224.1",
          "transcript_support_level": null,
          "aa_start": 317,
          "aa_end": null,
          "aa_length": 886,
          "cds_start": 950,
          "cds_end": null,
          "cds_length": 2661,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441295.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.938C>G",
          "hgvs_p": "p.Pro313Arg",
          "transcript": "NM_001441296.1",
          "protein_id": "NP_001428225.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 882,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 2649,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441296.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.917C>G",
          "hgvs_p": "p.Pro306Arg",
          "transcript": "ENST00000950081.1",
          "protein_id": "ENSP00000620140.1",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 875,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 2628,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950081.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.911C>G",
          "hgvs_p": "p.Pro304Arg",
          "transcript": "NM_001441297.1",
          "protein_id": "NP_001428226.1",
          "transcript_support_level": null,
          "aa_start": 304,
          "aa_end": null,
          "aa_length": 873,
          "cds_start": 911,
          "cds_end": null,
          "cds_length": 2622,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441297.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.875C>G",
          "hgvs_p": "p.Pro292Arg",
          "transcript": "NM_001441298.1",
          "protein_id": "NP_001428227.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 861,
          "cds_start": 875,
          "cds_end": null,
          "cds_length": 2586,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441298.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.875C>G",
          "hgvs_p": "p.Pro292Arg",
          "transcript": "ENST00000878905.1",
          "protein_id": "ENSP00000548964.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 861,
          "cds_start": 875,
          "cds_end": null,
          "cds_length": 2586,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878905.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.872C>G",
          "hgvs_p": "p.Pro291Arg",
          "transcript": "NM_001441299.1",
          "protein_id": "NP_001428228.1",
          "transcript_support_level": null,
          "aa_start": 291,
          "aa_end": null,
          "aa_length": 860,
          "cds_start": 872,
          "cds_end": null,
          "cds_length": 2583,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441299.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.872C>G",
          "hgvs_p": "p.Pro291Arg",
          "transcript": "ENST00000878904.1",
          "protein_id": "ENSP00000548963.1",
          "transcript_support_level": null,
          "aa_start": 291,
          "aa_end": null,
          "aa_length": 860,
          "cds_start": 872,
          "cds_end": null,
          "cds_length": 2583,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878904.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.989C>G",
          "hgvs_p": "p.Pro330Arg",
          "transcript": "ENST00000683197.1",
          "protein_id": "ENSP00000507641.1",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 989,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000683197.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.674C>G",
          "hgvs_p": "p.Pro225Arg",
          "transcript": "ENST00000950082.1",
          "protein_id": "ENSP00000620141.1",
          "transcript_support_level": null,
          "aa_start": 225,
          "aa_end": null,
          "aa_length": 794,
          "cds_start": 674,
          "cds_end": null,
          "cds_length": 2385,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950082.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.989C>G",
          "hgvs_p": "p.Pro330Arg",
          "transcript": "NM_001441300.1",
          "protein_id": "NP_001428229.1",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 989,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001441300.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO5",
          "gene_hgnc_id": 27337,
          "hgvs_c": "c.989C>G",
          "hgvs_p": "p.Pro330Arg",
          "transcript": "ENST00000939029.1",
          "protein_id": "ENSP00000609088.1",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 768,
          "cds_start": 989,
          "cds_end": null,
          "cds_length": 2307,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "dbsnp": "rs541372136",
      "frequency_reference_population": 0.00017782592,
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      "allele_count_reference_population": 287,
      "gnomad_exomes_af": 0.00018403,
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      "gnomad_genomes_ac": 18,
      "gnomad_exomes_homalt": 3,
      "gnomad_genomes_homalt": 0,
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      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.006363898515701294,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.146,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1282,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.735,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM1,BP4_Strong,BP6,BS2_Supporting",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM1",
            "BP4_Strong",
            "BP6",
            "BS2_Supporting"
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          "verdict": "Likely_benign",
          "transcript": "NM_213599.3",
          "gene_symbol": "ANO5",
          "hgnc_id": 27337,
          "effects": [
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          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1031C>G",
          "hgvs_p": "p.Pro344Arg"
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      ],
      "clinvar_disease": "Autosomal recessive limb-girdle muscular dystrophy type 2L,Gnathodiaphyseal dysplasia,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:3 LB:1",
      "phenotype_combined": "not provided|Gnathodiaphyseal dysplasia;Autosomal recessive limb-girdle muscular dystrophy type 2L",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}