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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 11-26599673-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=11&pos=26599673&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "11",
      "pos": 26599673,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001313726.2",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1795G>C",
          "hgvs_p": "p.Ala599Pro",
          "transcript": "NM_031418.4",
          "protein_id": "NP_113606.2",
          "transcript_support_level": null,
          "aa_start": 599,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 1795,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": 1941,
          "cdna_end": null,
          "cdna_length": 5937,
          "mane_select": "ENST00000256737.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_031418.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1795G>C",
          "hgvs_p": "p.Ala599Pro",
          "transcript": "ENST00000256737.8",
          "protein_id": "ENSP00000256737.3",
          "transcript_support_level": 1,
          "aa_start": 599,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 1795,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": 1941,
          "cdna_end": null,
          "cdna_length": 5937,
          "mane_select": "NM_031418.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000256737.8"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1978G>C",
          "hgvs_p": "p.Ala660Pro",
          "transcript": "NM_001313726.2",
          "protein_id": "NP_001300655.1",
          "transcript_support_level": null,
          "aa_start": 660,
          "aa_end": null,
          "aa_length": 1042,
          "cds_start": 1978,
          "cds_end": null,
          "cds_length": 3129,
          "cdna_start": 2347,
          "cdna_end": null,
          "cdna_length": 6343,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001313726.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1978G>C",
          "hgvs_p": "p.Ala660Pro",
          "transcript": "ENST00000672621.1",
          "protein_id": "ENSP00000500506.1",
          "transcript_support_level": null,
          "aa_start": 660,
          "aa_end": null,
          "aa_length": 1042,
          "cds_start": 1978,
          "cds_end": null,
          "cds_length": 3129,
          "cdna_start": 2313,
          "cdna_end": null,
          "cdna_length": 6308,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000672621.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1747G>C",
          "hgvs_p": "p.Ala583Pro",
          "transcript": "ENST00000525139.5",
          "protein_id": "ENSP00000432576.1",
          "transcript_support_level": 5,
          "aa_start": 583,
          "aa_end": null,
          "aa_length": 965,
          "cds_start": 1747,
          "cds_end": null,
          "cds_length": 2898,
          "cdna_start": 1870,
          "cdna_end": null,
          "cdna_length": 3257,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000525139.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1357G>C",
          "hgvs_p": "p.Ala453Pro",
          "transcript": "NM_001313727.2",
          "protein_id": "NP_001300656.1",
          "transcript_support_level": null,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1357,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": 1537,
          "cdna_end": null,
          "cdna_length": 5533,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001313727.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1357G>C",
          "hgvs_p": "p.Ala453Pro",
          "transcript": "ENST00000531568.1",
          "protein_id": "ENSP00000432394.1",
          "transcript_support_level": 2,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1357,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": 1590,
          "cdna_end": null,
          "cdna_length": 3117,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000531568.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1750G>C",
          "hgvs_p": "p.Ala584Pro",
          "transcript": "XM_047427399.1",
          "protein_id": "XP_047283355.1",
          "transcript_support_level": null,
          "aa_start": 584,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 1750,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": 1896,
          "cdna_end": null,
          "cdna_length": 5892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047427399.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1357G>C",
          "hgvs_p": "p.Ala453Pro",
          "transcript": "XM_017018118.3",
          "protein_id": "XP_016873607.1",
          "transcript_support_level": null,
          "aa_start": 453,
          "aa_end": null,
          "aa_length": 835,
          "cds_start": 1357,
          "cds_end": null,
          "cds_length": 2508,
          "cdna_start": 1568,
          "cdna_end": null,
          "cdna_length": 5564,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017018118.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.1066G>C",
          "hgvs_p": "p.Ala356Pro",
          "transcript": "XM_017018119.3",
          "protein_id": "XP_016873608.1",
          "transcript_support_level": null,
          "aa_start": 356,
          "aa_end": null,
          "aa_length": 738,
          "cds_start": 1066,
          "cds_end": null,
          "cds_length": 2217,
          "cdna_start": 1180,
          "cdna_end": null,
          "cdna_length": 5176,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017018119.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ANO3",
          "gene_hgnc_id": 14004,
          "hgvs_c": "c.397G>C",
          "hgvs_p": "p.Ala133Pro",
          "transcript": "XM_011520282.4",
          "protein_id": "XP_011518584.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 515,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 1548,
          "cdna_start": 554,
          "cdna_end": null,
          "cdna_length": 4550,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011520282.4"
        }
      ],
      "gene_symbol": "ANO3",
      "gene_hgnc_id": 14004,
      "dbsnp": "rs1554976231",
      "frequency_reference_population": 6.8409184e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84092e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6783190369606018,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.389,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.978,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.04,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 9.968,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001313726.2",
          "gene_symbol": "ANO3",
          "hgnc_id": 14004,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1978G>C",
          "hgvs_p": "p.Ala660Pro"
        }
      ],
      "clinvar_disease": "Dystonic disorder",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Dystonic disorder",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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