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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-6451307-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=6451307&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 6451307,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001413263.1",
      "consequences": [
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.698A>G",
          "hgvs_p": "p.His233Arg",
          "transcript": "NM_001242.5",
          "protein_id": "NP_001233.2",
          "transcript_support_level": null,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000266557.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001242.5"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.698A>G",
          "hgvs_p": "p.His233Arg",
          "transcript": "ENST00000266557.4",
          "protein_id": "ENSP00000266557.3",
          "transcript_support_level": 1,
          "aa_start": 233,
          "aa_end": null,
          "aa_length": 260,
          "cds_start": 698,
          "cds_end": null,
          "cds_length": 783,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001242.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000266557.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CD27-AS1",
          "gene_hgnc_id": 43896,
          "hgvs_c": "n.34+162T>C",
          "hgvs_p": null,
          "transcript": "ENST00000399492.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000399492.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CD27-AS1",
          "gene_hgnc_id": 43896,
          "hgvs_c": "n.152+162T>C",
          "hgvs_p": null,
          "transcript": "ENST00000504270.4",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000504270.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CD27-AS1",
          "gene_hgnc_id": 43896,
          "hgvs_c": "n.51+162T>C",
          "hgvs_p": null,
          "transcript": "ENST00000537003.2",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000537003.2"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.791A>G",
          "hgvs_p": "p.His264Arg",
          "transcript": "NM_001413263.1",
          "protein_id": "NP_001400192.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 791,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413263.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.791A>G",
          "hgvs_p": "p.His264Arg",
          "transcript": "ENST00000881848.1",
          "protein_id": "ENSP00000551907.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 791,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881848.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.713A>G",
          "hgvs_p": "p.His238Arg",
          "transcript": "ENST00000881847.1",
          "protein_id": "ENSP00000551906.1",
          "transcript_support_level": null,
          "aa_start": 238,
          "aa_end": null,
          "aa_length": 265,
          "cds_start": 713,
          "cds_end": null,
          "cds_length": 798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881847.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.671A>G",
          "hgvs_p": "p.His224Arg",
          "transcript": "NM_001413264.1",
          "protein_id": "NP_001400193.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413264.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.671A>G",
          "hgvs_p": "p.His224Arg",
          "transcript": "ENST00000881846.1",
          "protein_id": "ENSP00000551905.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000881846.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.518A>G",
          "hgvs_p": "p.His173Arg",
          "transcript": "NM_001413265.1",
          "protein_id": "NP_001400194.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": 518,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413265.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.248A>G",
          "hgvs_p": "p.His83Arg",
          "transcript": "NM_001413266.1",
          "protein_id": "NP_001400195.1",
          "transcript_support_level": null,
          "aa_start": 83,
          "aa_end": null,
          "aa_length": 110,
          "cds_start": 248,
          "cds_end": null,
          "cds_length": 333,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413266.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.248A>G",
          "hgvs_p": "p.His83Arg",
          "transcript": "NM_001413267.1",
          "protein_id": "NP_001400196.1",
          "transcript_support_level": null,
          "aa_start": 83,
          "aa_end": null,
          "aa_length": 110,
          "cds_start": 248,
          "cds_end": null,
          "cds_length": 333,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413267.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.248A>G",
          "hgvs_p": "p.His83Arg",
          "transcript": "NM_001413268.1",
          "protein_id": "NP_001400197.1",
          "transcript_support_level": null,
          "aa_start": 83,
          "aa_end": null,
          "aa_length": 110,
          "cds_start": 248,
          "cds_end": null,
          "cds_length": 333,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413268.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.560A>G",
          "hgvs_p": "p.His187Arg",
          "transcript": "XM_011521042.4",
          "protein_id": "XP_011519344.1",
          "transcript_support_level": null,
          "aa_start": 187,
          "aa_end": null,
          "aa_length": 214,
          "cds_start": 560,
          "cds_end": null,
          "cds_length": 645,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011521042.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.810A>G",
          "hgvs_p": "p.Ser270Ser",
          "transcript": "XM_017020234.2",
          "protein_id": "XP_016875723.1",
          "transcript_support_level": null,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 810,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017020234.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "c.672A>G",
          "hgvs_p": "p.Ser224Ser",
          "transcript": "XM_047429900.1",
          "protein_id": "XP_047285856.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 312,
          "cds_start": 672,
          "cds_end": null,
          "cds_length": 939,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047429900.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27-AS1",
          "gene_hgnc_id": 43896,
          "hgvs_c": "n.211T>C",
          "hgvs_p": null,
          "transcript": "ENST00000535639.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000535639.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27",
          "gene_hgnc_id": 11922,
          "hgvs_c": "n.634A>G",
          "hgvs_p": null,
          "transcript": "ENST00000541233.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000541233.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD27-AS1",
          "gene_hgnc_id": 43896,
          "hgvs_c": "n.213T>C",
          "hgvs_p": null,
          "transcript": "ENST00000762183.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000762183.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
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      "dbsnp": "rs2532502",
      "frequency_reference_population": 0.99068576,
      "hom_count_reference_population": 792098,
      "allele_count_reference_population": 1599056,
      "gnomad_exomes_af": 0.990382,
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      "gnomad_exomes_homalt": 716926,
      "gnomad_genomes_homalt": 75172,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 6.732140036547207e-7,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.25,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0624,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.4,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.374,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
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          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001413263.1",
          "gene_symbol": "CD27",
          "hgnc_id": 11922,
          "effects": [
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          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.791A>G",
          "hgvs_p": "p.His264Arg"
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        {
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          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000535639.5",
          "gene_symbol": "CD27-AS1",
          "hgnc_id": 43896,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.211T>C",
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      ],
      "clinvar_disease": "Lymphoproliferative syndrome 2,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:5",
      "phenotype_combined": "not specified|Lymphoproliferative syndrome 2|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}