← Back to variant description

GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 12-8648141-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=12&pos=8648141&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "12",
      "pos": 8648141,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "ENST00000359478.7",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.472C>T",
          "hgvs_p": "p.Arg158*",
          "transcript": "NM_003480.4",
          "protein_id": "NP_003471.1",
          "transcript_support_level": null,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 472,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": 674,
          "cdna_end": null,
          "cdna_length": 2872,
          "mane_select": "ENST00000359478.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.472C>T",
          "hgvs_p": "p.Arg158*",
          "transcript": "ENST00000359478.7",
          "protein_id": "ENSP00000352455.2",
          "transcript_support_level": 1,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 472,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": 674,
          "cdna_end": null,
          "cdna_length": 2872,
          "mane_select": "NM_003480.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.442C>T",
          "hgvs_p": "p.Arg148*",
          "transcript": "ENST00000540087.5",
          "protein_id": "ENSP00000440496.1",
          "transcript_support_level": 1,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 163,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 492,
          "cdna_start": 442,
          "cdna_end": null,
          "cdna_length": 492,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.442C>T",
          "hgvs_p": "p.Arg148*",
          "transcript": "NM_001297709.2",
          "protein_id": "NP_001284638.1",
          "transcript_support_level": null,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 163,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 492,
          "cdna_start": 644,
          "cdna_end": null,
          "cdna_length": 2842,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.442C>T",
          "hgvs_p": "p.Arg148*",
          "transcript": "ENST00000396549.6",
          "protein_id": "ENSP00000379798.2",
          "transcript_support_level": 2,
          "aa_start": 148,
          "aa_end": null,
          "aa_length": 163,
          "cds_start": 442,
          "cds_end": null,
          "cds_length": 492,
          "cdna_start": 644,
          "cdna_end": null,
          "cdna_length": 1195,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.439C>T",
          "hgvs_p": "p.Arg147*",
          "transcript": "ENST00000535411.5",
          "protein_id": "ENSP00000441185.1",
          "transcript_support_level": 4,
          "aa_start": 147,
          "aa_end": null,
          "aa_length": 162,
          "cds_start": 439,
          "cds_end": null,
          "cds_length": 489,
          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 585,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.406C>T",
          "hgvs_p": "p.Arg136*",
          "transcript": "NM_001297710.2",
          "protein_id": "NP_001284639.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 151,
          "cds_start": 406,
          "cds_end": null,
          "cds_length": 456,
          "cdna_start": 608,
          "cdna_end": null,
          "cdna_length": 2806,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.406C>T",
          "hgvs_p": "p.Arg136*",
          "transcript": "ENST00000543369.5",
          "protein_id": "ENSP00000441492.1",
          "transcript_support_level": 3,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 151,
          "cds_start": 406,
          "cds_end": null,
          "cds_length": 456,
          "cdna_start": 469,
          "cdna_end": null,
          "cdna_length": 972,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133*",
          "transcript": "NM_001297711.2",
          "protein_id": "NP_001284640.1",
          "transcript_support_level": null,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": 599,
          "cdna_end": null,
          "cdna_length": 2797,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.397C>T",
          "hgvs_p": "p.Arg133*",
          "transcript": "ENST00000433590.6",
          "protein_id": "ENSP00000411997.2",
          "transcript_support_level": 2,
          "aa_start": 133,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 397,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": 657,
          "cdna_end": null,
          "cdna_length": 2308,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.280C>T",
          "hgvs_p": "p.Arg94*",
          "transcript": "NM_001297712.2",
          "protein_id": "NP_001284641.1",
          "transcript_support_level": null,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 109,
          "cds_start": 280,
          "cds_end": null,
          "cds_length": 330,
          "cdna_start": 482,
          "cdna_end": null,
          "cdna_length": 2680,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.280C>T",
          "hgvs_p": "p.Arg94*",
          "transcript": "ENST00000535336.5",
          "protein_id": "ENSP00000438525.1",
          "transcript_support_level": 4,
          "aa_start": 94,
          "aa_end": null,
          "aa_length": 109,
          "cds_start": 280,
          "cds_end": null,
          "cds_length": 330,
          "cdna_start": 381,
          "cdna_end": null,
          "cdna_length": 566,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Arg64*",
          "transcript": "ENST00000543467.5",
          "protein_id": "ENSP00000444531.1",
          "transcript_support_level": 3,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 79,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 240,
          "cdna_start": 190,
          "cdna_end": null,
          "cdna_length": 388,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.*124C>T",
          "hgvs_p": null,
          "transcript": "ENST00000537009.5",
          "protein_id": "ENSP00000439289.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 620,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.431C>T",
          "hgvs_p": null,
          "transcript": "ENST00000538694.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.*124C>T",
          "hgvs_p": null,
          "transcript": "ENST00000544211.5",
          "protein_id": "ENSP00000443839.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 840,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.743C>T",
          "hgvs_p": null,
          "transcript": "NR_123733.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2941,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.713C>T",
          "hgvs_p": null,
          "transcript": "NR_123734.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2911,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.*124C>T",
          "hgvs_p": null,
          "transcript": "ENST00000537009.5",
          "protein_id": "ENSP00000439289.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 620,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MFAP5",
          "gene_hgnc_id": 29673,
          "hgvs_c": "n.*124C>T",
          "hgvs_p": null,
          "transcript": "ENST00000544211.5",
          "protein_id": "ENSP00000443839.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 840,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MFAP5",
      "gene_hgnc_id": 29673,
      "dbsnp": "rs727502791",
      "frequency_reference_population": 0.000020523401,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 30,
      "gnomad_exomes_af": 0.0000205234,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 30,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.4699999988079071,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.47,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 2.096,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PVS1_Moderate,PP5,BS2",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 4,
          "pathogenic_score": 3,
          "criteria": [
            "PVS1_Moderate",
            "PP5",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000359478.7",
          "gene_symbol": "MFAP5",
          "hgnc_id": 29673,
          "effects": [
            "stop_gained"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.472C>T",
          "hgvs_p": "p.Arg158*"
        }
      ],
      "clinvar_disease": " familial thoracic 9,Aortic aneurysm,Isolated thoracic aortic aneurysm,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:3 LP:2 US:1",
      "phenotype_combined": "Aortic aneurysm, familial thoracic 9|Isolated thoracic aortic aneurysm|not provided",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}