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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 14-77311960-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=14&pos=77311960&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "14",
      "pos": 77311960,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000261534.9",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "NM_013382.7",
          "protein_id": "NP_037514.2",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 750,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2253,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4875,
          "mane_select": "ENST00000261534.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "ENST00000261534.9",
          "protein_id": "ENSP00000261534.4",
          "transcript_support_level": 1,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 750,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2253,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4875,
          "mane_select": "NM_013382.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.249-5519C>T",
          "hgvs_p": null,
          "transcript": "ENST00000556326.5",
          "protein_id": "ENSP00000450630.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 948,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "ENST00000682795.1",
          "protein_id": "ENSP00000507574.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 799,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2400,
          "cdna_start": 382,
          "cdna_end": null,
          "cdna_length": 2828,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "ENST00000682247.1",
          "protein_id": "ENSP00000507213.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 774,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2325,
          "cdna_start": 443,
          "cdna_end": null,
          "cdna_length": 2729,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "ENST00000682467.1",
          "protein_id": "ENSP00000508062.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 703,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2112,
          "cdna_start": 468,
          "cdna_end": null,
          "cdna_length": 4604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_047431312.1",
          "protein_id": "XP_047287268.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 826,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2481,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 5103,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_011536675.3",
          "protein_id": "XP_011534977.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 813,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2442,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 5064,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_047431313.1",
          "protein_id": "XP_047287269.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4914,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_047431315.1",
          "protein_id": "XP_047287271.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 685,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2058,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 2323,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_047431316.1",
          "protein_id": "XP_047287272.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4644,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_011536677.4",
          "protein_id": "XP_011534979.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 660,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 1983,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4605,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser",
          "transcript": "XM_047431318.1",
          "protein_id": "XP_047287274.1",
          "transcript_support_level": null,
          "aa_start": 108,
          "aa_end": null,
          "aa_length": 597,
          "cds_start": 322,
          "cds_end": null,
          "cds_length": 1794,
          "cdna_start": 524,
          "cdna_end": null,
          "cdna_length": 4416,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.345C>T",
          "hgvs_p": null,
          "transcript": "ENST00000452340.7",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5647,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.203C>T",
          "hgvs_p": null,
          "transcript": "ENST00000554948.1",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 565,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.156C>T",
          "hgvs_p": null,
          "transcript": "ENST00000555788.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 470,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.412C>T",
          "hgvs_p": null,
          "transcript": "ENST00000557525.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 556,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.268C>T",
          "hgvs_p": null,
          "transcript": "ENST00000682382.1",
          "protein_id": "ENSP00000507975.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2995,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.115C>T",
          "hgvs_p": null,
          "transcript": "ENST00000683188.1",
          "protein_id": "ENSP00000507734.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2852,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.190C>T",
          "hgvs_p": null,
          "transcript": "ENST00000683828.1",
          "protein_id": "ENSP00000507134.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1686,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "POMT2",
          "gene_hgnc_id": 19743,
          "hgvs_c": "n.68C>T",
          "hgvs_p": null,
          "transcript": "ENST00000684102.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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        },
        {
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          ],
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          "gene_symbol": "POMT2",
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          "hgvs_c": "c.249-7160C>T",
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          "protein_id": "ENSP00000451967.2",
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        },
        {
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          ],
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          "gene_symbol": "POMT2",
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          "hgvs_c": "c.1-5519C>T",
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          "transcript": "XM_047431314.1",
          "protein_id": "XP_047287270.1",
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          "cdna_start": null,
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          "cdna_length": 5018,
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        }
      ],
      "gene_symbol": "POMT2",
      "gene_hgnc_id": 19743,
      "dbsnp": "rs1555355402",
      "frequency_reference_population": 0.0000013681781,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000136818,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9367971420288086,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.933,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9401,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.44,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.482,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 4,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000261534.9",
          "gene_symbol": "POMT2",
          "hgnc_id": 19743,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.322C>T",
          "hgvs_p": "p.Pro108Ser"
        }
      ],
      "clinvar_disease": " type A2, type B2,Autosomal recessive limb-girdle muscular dystrophy type 2N,Muscular dystrophy-dystroglycanopathy (congenital with brain and eye anomalies),Muscular dystrophy-dystroglycanopathy (congenital with intellectual disability)",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Autosomal recessive limb-girdle muscular dystrophy type 2N;Muscular dystrophy-dystroglycanopathy (congenital with brain and eye anomalies), type A2;Muscular dystrophy-dystroglycanopathy (congenital with intellectual disability), type B2",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}