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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 15-28130164-A-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=15&pos=28130164&ref=A&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "15",
      "pos": 28130164,
      "ref": "A",
      "alt": "T",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_004667.6",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 83,
          "exon_rank_end": null,
          "exon_count": 93,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12801T>A",
          "hgvs_p": "p.Asp4267Glu",
          "transcript": "NM_004667.6",
          "protein_id": "NP_004658.3",
          "transcript_support_level": null,
          "aa_start": 4267,
          "aa_end": null,
          "aa_length": 4834,
          "cds_start": 12801,
          "cds_end": null,
          "cds_length": 14505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000261609.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004667.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 83,
          "exon_rank_end": null,
          "exon_count": 93,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12801T>A",
          "hgvs_p": "p.Asp4267Glu",
          "transcript": "ENST00000261609.13",
          "protein_id": "ENSP00000261609.8",
          "transcript_support_level": 1,
          "aa_start": 4267,
          "aa_end": null,
          "aa_length": 4834,
          "cds_start": 12801,
          "cds_end": null,
          "cds_length": 14505,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004667.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261609.13"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 83,
          "exon_rank_end": null,
          "exon_count": 93,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12786T>A",
          "hgvs_p": "p.Asp4262Glu",
          "transcript": "XM_006720726.4",
          "protein_id": "XP_006720789.1",
          "transcript_support_level": null,
          "aa_start": 4262,
          "aa_end": null,
          "aa_length": 4829,
          "cds_start": 12786,
          "cds_end": null,
          "cds_length": 14490,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006720726.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 82,
          "exon_rank_end": null,
          "exon_count": 92,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12774T>A",
          "hgvs_p": "p.Asp4258Glu",
          "transcript": "XM_047433206.1",
          "protein_id": "XP_047289162.1",
          "transcript_support_level": null,
          "aa_start": 4258,
          "aa_end": null,
          "aa_length": 4825,
          "cds_start": 12774,
          "cds_end": null,
          "cds_length": 14478,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433206.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 82,
          "exon_rank_end": null,
          "exon_count": 92,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12687T>A",
          "hgvs_p": "p.Asp4229Glu",
          "transcript": "XM_005268276.6",
          "protein_id": "XP_005268333.1",
          "transcript_support_level": null,
          "aa_start": 4229,
          "aa_end": null,
          "aa_length": 4796,
          "cds_start": 12687,
          "cds_end": null,
          "cds_length": 14391,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005268276.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 82,
          "exon_rank_end": null,
          "exon_count": 92,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12687T>A",
          "hgvs_p": "p.Asp4229Glu",
          "transcript": "XM_017022695.1",
          "protein_id": "XP_016878184.1",
          "transcript_support_level": null,
          "aa_start": 4229,
          "aa_end": null,
          "aa_length": 4796,
          "cds_start": 12687,
          "cds_end": null,
          "cds_length": 14391,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022695.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 82,
          "exon_rank_end": null,
          "exon_count": 92,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12687T>A",
          "hgvs_p": "p.Asp4229Glu",
          "transcript": "XM_017022696.2",
          "protein_id": "XP_016878185.1",
          "transcript_support_level": null,
          "aa_start": 4229,
          "aa_end": null,
          "aa_length": 4796,
          "cds_start": 12687,
          "cds_end": null,
          "cds_length": 14391,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022696.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 81,
          "exon_rank_end": null,
          "exon_count": 91,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12543T>A",
          "hgvs_p": "p.Asp4181Glu",
          "transcript": "XM_006720727.4",
          "protein_id": "XP_006720790.1",
          "transcript_support_level": null,
          "aa_start": 4181,
          "aa_end": null,
          "aa_length": 4748,
          "cds_start": 12543,
          "cds_end": null,
          "cds_length": 14247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006720727.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 79,
          "exon_rank_end": null,
          "exon_count": 89,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.12318T>A",
          "hgvs_p": "p.Asp4106Glu",
          "transcript": "XM_047433207.1",
          "protein_id": "XP_047289163.1",
          "transcript_support_level": null,
          "aa_start": 4106,
          "aa_end": null,
          "aa_length": 4673,
          "cds_start": 12318,
          "cds_end": null,
          "cds_length": 14022,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433207.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 52,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.5967T>A",
          "hgvs_p": "p.Asp1989Glu",
          "transcript": "XM_017022697.2",
          "protein_id": "XP_016878186.1",
          "transcript_support_level": null,
          "aa_start": 1989,
          "aa_end": null,
          "aa_length": 2556,
          "cds_start": 5967,
          "cds_end": null,
          "cds_length": 7671,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022697.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 52,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "c.5967T>A",
          "hgvs_p": "p.Asp1989Glu",
          "transcript": "XM_017022698.2",
          "protein_id": "XP_016878187.1",
          "transcript_support_level": null,
          "aa_start": 1989,
          "aa_end": null,
          "aa_length": 2556,
          "cds_start": 5967,
          "cds_end": null,
          "cds_length": 7671,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022698.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 39,
          "intron_rank": 29,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "n.4281+1936T>A",
          "hgvs_p": null,
          "transcript": "ENST00000650509.1",
          "protein_id": "ENSP00000496936.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000650509.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HERC2",
          "gene_hgnc_id": 4868,
          "hgvs_c": "n.*136T>A",
          "hgvs_p": null,
          "transcript": "ENST00000649023.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000649023.1"
        }
      ],
      "gene_symbol": "HERC2",
      "gene_hgnc_id": 4868,
      "dbsnp": "rs373175587",
      "frequency_reference_population": 0.00089719327,
      "hom_count_reference_population": 23,
      "allele_count_reference_population": 1448,
      "gnomad_exomes_af": 0.000912531,
      "gnomad_genomes_af": 0.000749734,
      "gnomad_exomes_ac": 1334,
      "gnomad_genomes_ac": 114,
      "gnomad_exomes_homalt": 21,
      "gnomad_genomes_homalt": 2,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.015111774206161499,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.18199999630451202,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.318,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0748,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.33,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.044,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.0000326579991845859,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_004667.6",
          "gene_symbol": "HERC2",
          "hgnc_id": 4868,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.12801T>A",
          "hgvs_p": "p.Asp4267Glu"
        }
      ],
      "clinvar_disease": "Developmental delay with autism spectrum disorder and gait instability,Inborn genetic diseases,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:3",
      "phenotype_combined": "not specified|Developmental delay with autism spectrum disorder and gait instability|Inborn genetic diseases|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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