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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-189998-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=189998&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 189998,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_201412.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "NM_201412.3",
          "protein_id": "NP_958815.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000293872.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_201412.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "ENST00000293872.13",
          "protein_id": "ENSP00000293872.8",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 371,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 1116,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_201412.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000293872.13"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "ENST00000337351.8",
          "protein_id": "ENSP00000337507.4",
          "transcript_support_level": 1,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000337351.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "n.*2107G>A",
          "hgvs_p": null,
          "transcript": "ENST00000426094.5",
          "protein_id": "ENSP00000390953.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000426094.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "n.2263G>A",
          "hgvs_p": null,
          "transcript": "ENST00000490762.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000490762.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "n.*2107G>A",
          "hgvs_p": null,
          "transcript": "ENST00000426094.5",
          "protein_id": "ENSP00000390953.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000426094.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.914G>A",
          "hgvs_p": "p.Arg305Gln",
          "transcript": "ENST00000862883.1",
          "protein_id": "ENSP00000532942.1",
          "transcript_support_level": null,
          "aa_start": 305,
          "aa_end": null,
          "aa_length": 361,
          "cds_start": 914,
          "cds_end": null,
          "cds_length": 1086,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000862883.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.845G>A",
          "hgvs_p": "p.Arg282Gln",
          "transcript": "ENST00000862884.1",
          "protein_id": "ENSP00000532943.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000862884.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.833G>A",
          "hgvs_p": "p.Arg278Gln",
          "transcript": "ENST00000920890.1",
          "protein_id": "ENSP00000590949.1",
          "transcript_support_level": null,
          "aa_start": 278,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 833,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920890.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.815G>A",
          "hgvs_p": "p.Arg272Gln",
          "transcript": "ENST00000920887.1",
          "protein_id": "ENSP00000590946.1",
          "transcript_support_level": null,
          "aa_start": 272,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 815,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920887.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "NM_001320226.2",
          "protein_id": "NP_001307155.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001320226.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "NM_018032.5",
          "protein_id": "NP_060502.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018032.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln",
          "transcript": "ENST00000397783.5",
          "protein_id": "ENSP00000380885.1",
          "transcript_support_level": 2,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 325,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 978,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000397783.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.800G>A",
          "hgvs_p": "p.Arg267Gln",
          "transcript": "ENST00000920888.1",
          "protein_id": "ENSP00000590947.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 323,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 972,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920888.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.785G>A",
          "hgvs_p": "p.Arg262Gln",
          "transcript": "NM_001330420.2",
          "protein_id": "NP_001317349.1",
          "transcript_support_level": null,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 272,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 819,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330420.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.785G>A",
          "hgvs_p": "p.Arg262Gln",
          "transcript": "ENST00000397780.5",
          "protein_id": "ENSP00000380882.1",
          "transcript_support_level": 5,
          "aa_start": 262,
          "aa_end": null,
          "aa_length": 272,
          "cds_start": 785,
          "cds_end": null,
          "cds_length": 819,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000397780.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.512G>A",
          "hgvs_p": "p.Arg171Gln",
          "transcript": "ENST00000920891.1",
          "protein_id": "ENSP00000590950.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
          "aa_length": 227,
          "cds_start": 512,
          "cds_end": null,
          "cds_length": 684,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920891.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.341G>A",
          "hgvs_p": "p.Arg114Gln",
          "transcript": "ENST00000429378.5",
          "protein_id": "ENSP00000413033.1",
          "transcript_support_level": 3,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 170,
          "cds_start": 341,
          "cds_end": null,
          "cds_length": 513,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000429378.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.146G>A",
          "hgvs_p": "p.Arg49Gln",
          "transcript": "ENST00000442701.1",
          "protein_id": "ENSP00000407781.1",
          "transcript_support_level": 3,
          "aa_start": 49,
          "aa_end": null,
          "aa_length": 59,
          "cds_start": 146,
          "cds_end": null,
          "cds_length": 180,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000442701.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LUC7L",
          "gene_hgnc_id": 6723,
          "hgvs_c": "c.845G>A",
          "hgvs_p": "p.Arg282Gln",
          "transcript": "XM_011522561.3",
          "protein_id": "XP_011520863.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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      ],
      "gene_symbol": "LUC7L",
      "gene_hgnc_id": 6723,
      "dbsnp": "rs146490197",
      "frequency_reference_population": 0.000009915938,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 16,
      "gnomad_exomes_af": 0.00000821109,
      "gnomad_genomes_af": 0.000026294,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.17156943678855896,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07000000029802322,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.059,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1065,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.31,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.979,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.07,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_201412.3",
          "gene_symbol": "LUC7L",
          "hgnc_id": 6723,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.944G>A",
          "hgvs_p": "p.Arg315Gln"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}