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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-3068004-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=3068004&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 3068004,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001308078.4",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.135G>C",
          "hgvs_p": "p.Glu45Asp",
          "transcript": "NM_001376923.1",
          "protein_id": "NP_001363852.1",
          "transcript_support_level": null,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 135,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000525643.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001376923.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.135G>C",
          "hgvs_p": "p.Glu45Asp",
          "transcript": "ENST00000525643.7",
          "protein_id": "ENSP00000432218.3",
          "transcript_support_level": 1,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 135,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001376923.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000525643.7"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000396890.6",
          "protein_id": "ENSP00000380099.2",
          "transcript_support_level": 1,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396890.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.135G>C",
          "hgvs_p": "p.Glu45Asp",
          "transcript": "ENST00000325568.9",
          "protein_id": "ENSP00000324742.5",
          "transcript_support_level": 1,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 188,
          "cds_start": 135,
          "cds_end": null,
          "cds_length": 567,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000325568.9"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.105G>C",
          "hgvs_p": "p.Glu35Asp",
          "transcript": "ENST00000531965.5",
          "protein_id": "ENSP00000433177.1",
          "transcript_support_level": 1,
          "aa_start": 35,
          "aa_end": null,
          "aa_length": 178,
          "cds_start": 105,
          "cds_end": null,
          "cds_length": 537,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000531965.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.135G>C",
          "hgvs_p": "p.Glu45Asp",
          "transcript": "ENST00000529699.5",
          "protein_id": "ENSP00000436937.1",
          "transcript_support_level": 1,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 168,
          "cds_start": 135,
          "cds_end": null,
          "cds_length": 507,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000529699.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.75G>C",
          "hgvs_p": "p.Glu25Asp",
          "transcript": "ENST00000548246.1",
          "protein_id": "ENSP00000447979.1",
          "transcript_support_level": 1,
          "aa_start": 25,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 75,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000548246.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.135G>C",
          "hgvs_p": "p.Glu45Asp",
          "transcript": "ENST00000396887.7",
          "protein_id": "ENSP00000380096.3",
          "transcript_support_level": 1,
          "aa_start": 45,
          "aa_end": null,
          "aa_length": 131,
          "cds_start": 135,
          "cds_end": null,
          "cds_length": 396,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000396887.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "n.720G>C",
          "hgvs_p": null,
          "transcript": "ENST00000532247.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000532247.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "NM_001308078.4",
          "protein_id": "NP_001295007.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001308078.4"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "NM_001369587.3",
          "protein_id": "NP_001356516.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369587.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "NM_001369588.3",
          "protein_id": "NP_001356517.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369588.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000534507.5",
          "protein_id": "ENSP00000431775.1",
          "transcript_support_level": 2,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000534507.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000548476.5",
          "protein_id": "ENSP00000449483.1",
          "transcript_support_level": 2,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000548476.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878151.1",
          "protein_id": "ENSP00000548210.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878151.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878152.1",
          "protein_id": "ENSP00000548211.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878152.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878157.1",
          "protein_id": "ENSP00000548216.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878157.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878158.1",
          "protein_id": "ENSP00000548217.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878158.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878159.1",
          "protein_id": "ENSP00000548218.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878159.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "IL32",
          "gene_hgnc_id": 16830,
          "hgvs_c": "c.273G>C",
          "hgvs_p": "p.Glu91Asp",
          "transcript": "ENST00000878165.1",
          "protein_id": "ENSP00000548224.1",
          "transcript_support_level": null,
          "aa_start": 91,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 273,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878165.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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      "computational_score_selected": 0.05306386947631836,
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      "computational_source_selected": "MetaRNN",
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Benign",
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      "bayesdelnoaf_score": -0.48,
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      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
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          "pathogenic_score": 2,
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            "BP4_Strong"
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          "verdict": "Likely_benign",
          "transcript": "NM_001308078.4",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}