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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-399659-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=399659&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 399659,
      "ref": "G",
      "alt": "C",
      "effect": "synonymous_variant",
      "transcript": "ENST00000219479.7",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.360G>C",
          "hgvs_p": "p.Ser120Ser",
          "transcript": "NM_005009.3",
          "protein_id": "NP_005000.1",
          "transcript_support_level": null,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": 360,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": 384,
          "cdna_end": null,
          "cdna_length": 1000,
          "mane_select": "ENST00000219479.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.360G>C",
          "hgvs_p": "p.Ser120Ser",
          "transcript": "ENST00000219479.7",
          "protein_id": "ENSP00000219479.2",
          "transcript_support_level": 1,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": 360,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": 384,
          "cdna_end": null,
          "cdna_length": 1000,
          "mane_select": "NM_005009.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.384G>C",
          "hgvs_p": "p.Ser128Ser",
          "transcript": "ENST00000382940.8",
          "protein_id": "ENSP00000372398.4",
          "transcript_support_level": 3,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 195,
          "cds_start": 384,
          "cds_end": null,
          "cds_length": 588,
          "cdna_start": 398,
          "cdna_end": null,
          "cdna_length": 766,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.360G>C",
          "hgvs_p": "p.Ser120Ser",
          "transcript": "NM_001286435.2",
          "protein_id": "NP_001273364.1",
          "transcript_support_level": null,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 360,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": 384,
          "cdna_end": null,
          "cdna_length": 1110,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.345G>C",
          "hgvs_p": "p.Ser115Ser",
          "transcript": "ENST00000433358.5",
          "protein_id": "ENSP00000405925.1",
          "transcript_support_level": 3,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 168,
          "cds_start": 345,
          "cds_end": null,
          "cds_length": 507,
          "cdna_start": 346,
          "cdna_end": null,
          "cdna_length": 966,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.258G>C",
          "hgvs_p": "p.Ser86Ser",
          "transcript": "NM_001286433.2",
          "protein_id": "NP_001273362.1",
          "transcript_support_level": null,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 258,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": 282,
          "cdna_end": null,
          "cdna_length": 898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.258G>C",
          "hgvs_p": "p.Ser86Ser",
          "transcript": "ENST00000621774.4",
          "protein_id": "ENSP00000479681.1",
          "transcript_support_level": 3,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 258,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": 289,
          "cdna_end": null,
          "cdna_length": 904,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "NM_001286436.2",
          "protein_id": "NP_001273365.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 620,
          "cdna_end": null,
          "cdna_length": 1236,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "NM_001286438.2",
          "protein_id": "NP_001273367.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 562,
          "cdna_end": null,
          "cdna_length": 1178,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "NM_001286439.2",
          "protein_id": "NP_001273368.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 571,
          "cdna_end": null,
          "cdna_length": 1187,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "NM_001286440.2",
          "protein_id": "NP_001273369.1",
          "transcript_support_level": null,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 707,
          "cdna_end": null,
          "cdna_length": 1323,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "ENST00000397722.5",
          "protein_id": "ENSP00000380834.1",
          "transcript_support_level": 2,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 624,
          "cdna_end": null,
          "cdna_length": 1232,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "ENST00000450036.1",
          "protein_id": "ENSP00000389048.1",
          "transcript_support_level": 3,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 707,
          "cdna_end": null,
          "cdna_length": 985,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.150G>C",
          "hgvs_p": "p.Ser50Ser",
          "transcript": "ENST00000620944.4",
          "protein_id": "ENSP00000479996.1",
          "transcript_support_level": 2,
          "aa_start": 50,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 150,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 578,
          "cdna_end": null,
          "cdna_length": 1193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "n.360G>C",
          "hgvs_p": null,
          "transcript": "ENST00000444498.5",
          "protein_id": "ENSP00000395605.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1111,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "n.*412G>C",
          "hgvs_p": null,
          "transcript": "ENST00000448828.5",
          "protein_id": "ENSP00000412039.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1167,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "n.1157G>C",
          "hgvs_p": null,
          "transcript": "ENST00000460297.1",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1652,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "n.1250G>C",
          "hgvs_p": null,
          "transcript": "ENST00000468031.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1861,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "n.*412G>C",
          "hgvs_p": null,
          "transcript": "ENST00000448828.5",
          "protein_id": "ENSP00000412039.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1167,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NME4",
          "gene_hgnc_id": 7852,
          "hgvs_c": "c.*185G>C",
          "hgvs_p": null,
          "transcript": "ENST00000454619.5",
          "protein_id": "ENSP00000406317.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 36,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 111,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 719,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "NME4",
      "gene_hgnc_id": 7852,
      "dbsnp": "rs14293",
      "frequency_reference_population": 0.000002738008,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000273801,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.004000000189989805,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "REVEL",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.004,
      "revel_prediction": "Benign",
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.88,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -3.163,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP7",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 5,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000219479.7",
          "gene_symbol": "NME4",
          "hgnc_id": 7852,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.360G>C",
          "hgvs_p": "p.Ser120Ser"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}