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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-70255828-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=70255828&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 70255828,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001605.3",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "NM_001605.3",
          "protein_id": "NP_001596.2",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000261772.13",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001605.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000261772.13",
          "protein_id": "ENSP00000261772.8",
          "transcript_support_level": 1,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001605.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000261772.13"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000565361.3",
          "protein_id": "ENSP00000455360.3",
          "transcript_support_level": 5,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 999,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 3000,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000565361.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000896288.1",
          "protein_id": "ENSP00000566347.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 999,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 3000,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896288.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000675853.1",
          "protein_id": "ENSP00000502367.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675853.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000951926.1",
          "protein_id": "ENSP00000621985.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951926.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2213G>A",
          "hgvs_p": "p.Arg738Gln",
          "transcript": "ENST00000675045.1",
          "protein_id": "ENSP00000502014.1",
          "transcript_support_level": null,
          "aa_start": 738,
          "aa_end": null,
          "aa_length": 977,
          "cds_start": 2213,
          "cds_end": null,
          "cds_length": 2934,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675045.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000674691.1",
          "protein_id": "ENSP00000502247.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674691.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000674963.1",
          "protein_id": "ENSP00000501924.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674963.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000675643.1",
          "protein_id": "ENSP00000502797.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000675643.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000896284.1",
          "protein_id": "ENSP00000566343.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896284.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000896291.1",
          "protein_id": "ENSP00000566350.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000896291.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000934581.1",
          "protein_id": "ENSP00000604640.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
          "cds_end": null,
          "cds_length": 2907,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000934581.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000934584.1",
          "protein_id": "ENSP00000604643.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
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          "cds_start": 2186,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          ],
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          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln",
          "transcript": "ENST00000951922.1",
          "protein_id": "ENSP00000621981.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 2186,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000951922.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2183G>A",
          "hgvs_p": "p.Arg728Gln",
          "transcript": "ENST00000951923.1",
          "protein_id": "ENSP00000621982.1",
          "transcript_support_level": null,
          "aa_start": 728,
          "aa_end": null,
          "aa_length": 967,
          "cds_start": 2183,
          "cds_end": null,
          "cds_length": 2904,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000951923.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2177G>A",
          "hgvs_p": "p.Arg726Gln",
          "transcript": "ENST00000951924.1",
          "protein_id": "ENSP00000621983.1",
          "transcript_support_level": null,
          "aa_start": 726,
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          "biotype": "protein_coding",
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        },
        {
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          ],
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          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2171G>A",
          "hgvs_p": "p.Arg724Gln",
          "transcript": "ENST00000896285.1",
          "protein_id": "ENSP00000566344.1",
          "transcript_support_level": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "protein_coding": true,
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          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2165G>A",
          "hgvs_p": "p.Arg722Gln",
          "transcript": "ENST00000674512.1",
          "protein_id": "ENSP00000501613.1",
          "transcript_support_level": null,
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          "cds_start": 2165,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000674512.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AARS1",
          "gene_hgnc_id": 20,
          "hgvs_c": "c.2159G>A",
          "hgvs_p": "p.Arg720Gln",
          "transcript": "ENST00000675133.1",
          "protein_id": "ENSP00000502230.1",
          "transcript_support_level": null,
          "aa_start": 720,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2159,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": null,
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      ],
      "gene_symbol": "AARS1",
      "gene_hgnc_id": 20,
      "dbsnp": "rs142850278",
      "frequency_reference_population": 0.000048963888,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 79,
      "gnomad_exomes_af": 0.0000485925,
      "gnomad_genomes_af": 0.0000525272,
      "gnomad_exomes_ac": 71,
      "gnomad_genomes_ac": 8,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.08809679746627808,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.077,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0746,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.46,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.701,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BP6",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001605.3",
          "gene_symbol": "AARS1",
          "hgnc_id": 20,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR,Unknown",
          "hgvs_c": "c.2186G>A",
          "hgvs_p": "p.Arg729Gln"
        }
      ],
      "clinvar_disease": " 29,Charcot-Marie-Tooth disease axonal type 2N,Charcot-Marie-Tooth disease type 2,Developmental and epileptic encephalopathy,Inborn genetic diseases,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:2",
      "phenotype_combined": "not provided|Charcot-Marie-Tooth disease type 2|Charcot-Marie-Tooth disease axonal type 2N;Developmental and epileptic encephalopathy, 29|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}