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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-81908570-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=81908570&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 81908570,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_002661.5",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "NM_002661.5",
          "protein_id": "NP_002652.2",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000564138.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002661.5"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000564138.6",
          "protein_id": "ENSP00000482457.1",
          "transcript_support_level": 1,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002661.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000564138.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.1956A>G",
          "hgvs_p": null,
          "transcript": "ENST00000567980.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000567980.5"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1865A>G",
          "hgvs_p": "p.Asn622Ser",
          "transcript": "ENST00000902427.1",
          "protein_id": "ENSP00000572486.1",
          "transcript_support_level": null,
          "aa_start": 622,
          "aa_end": null,
          "aa_length": 1316,
          "cds_start": 1865,
          "cds_end": null,
          "cds_length": 3951,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902427.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "NM_001425749.1",
          "protein_id": "NP_001412678.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425749.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "NM_001425750.1",
          "protein_id": "NP_001412679.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425750.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "NM_001425751.1",
          "protein_id": "NP_001412680.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001425751.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000565054.7",
          "protein_id": "ENSP00000520638.1",
          "transcript_support_level": 5,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000565054.7"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000697580.2",
          "protein_id": "ENSP00000520637.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697580.2"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000902425.1",
          "protein_id": "ENSP00000572484.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902425.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000902431.1",
          "protein_id": "ENSP00000572490.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902431.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser",
          "transcript": "ENST00000902429.1",
          "protein_id": "ENSP00000572488.1",
          "transcript_support_level": null,
          "aa_start": 571,
          "aa_end": null,
          "aa_length": 1263,
          "cds_start": 1712,
          "cds_end": null,
          "cds_length": 3792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902429.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1703A>G",
          "hgvs_p": "p.Asn568Ser",
          "transcript": "ENST00000902430.1",
          "protein_id": "ENSP00000572489.1",
          "transcript_support_level": null,
          "aa_start": 568,
          "aa_end": null,
          "aa_length": 1262,
          "cds_start": 1703,
          "cds_end": null,
          "cds_length": 3789,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902430.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1676A>G",
          "hgvs_p": "p.Asn559Ser",
          "transcript": "ENST00000902428.1",
          "protein_id": "ENSP00000572487.1",
          "transcript_support_level": null,
          "aa_start": 559,
          "aa_end": null,
          "aa_length": 1253,
          "cds_start": 1676,
          "cds_end": null,
          "cds_length": 3762,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902428.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1607A>G",
          "hgvs_p": "p.Asn536Ser",
          "transcript": "ENST00000902426.1",
          "protein_id": "ENSP00000572485.1",
          "transcript_support_level": null,
          "aa_start": 536,
          "aa_end": null,
          "aa_length": 1230,
          "cds_start": 1607,
          "cds_end": null,
          "cds_length": 3693,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902426.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1595A>G",
          "hgvs_p": "p.Asn532Ser",
          "transcript": "ENST00000697564.1",
          "protein_id": "ENSP00000513340.1",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 1226,
          "cds_start": 1595,
          "cds_end": null,
          "cds_length": 3681,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697564.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1568A>G",
          "hgvs_p": "p.Asn523Ser",
          "transcript": "ENST00000947323.1",
          "protein_id": "ENSP00000617382.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 1217,
          "cds_start": 1568,
          "cds_end": null,
          "cds_length": 3654,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947323.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1511A>G",
          "hgvs_p": "p.Asn504Ser",
          "transcript": "ENST00000697583.1",
          "protein_id": "ENSP00000513349.1",
          "transcript_support_level": null,
          "aa_start": 504,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 1511,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697583.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1511A>G",
          "hgvs_p": "p.Asn504Ser",
          "transcript": "ENST00000697584.1",
          "protein_id": "ENSP00000513350.1",
          "transcript_support_level": null,
          "aa_start": 504,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 1511,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000697584.1"
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.1511A>G",
          "hgvs_p": "p.Asn504Ser",
          "transcript": "ENST00000697585.1",
          "protein_id": "ENSP00000513351.1",
          "transcript_support_level": null,
          "aa_start": 504,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 1511,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": null,
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        {
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000697581.1"
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      ],
      "gene_symbol": "PLCG2",
      "gene_hgnc_id": 9066,
      "dbsnp": "rs75472618",
      "frequency_reference_population": 0.0065914867,
      "hom_count_reference_population": 73,
      "allele_count_reference_population": 10624,
      "gnomad_exomes_af": 0.00646158,
      "gnomad_genomes_af": 0.00783703,
      "gnomad_exomes_ac": 9431,
      "gnomad_genomes_ac": 1193,
      "gnomad_exomes_homalt": 65,
      "gnomad_genomes_homalt": 8,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.007156580686569214,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.293,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.069,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.357,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_002661.5",
          "gene_symbol": "PLCG2",
          "hgnc_id": 9066,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1712A>G",
          "hgvs_p": "p.Asn571Ser"
        }
      ],
      "clinvar_disease": "Autoinflammation-PLCG2-associated antibody deficiency-immune dysregulation,Familial cold autoinflammatory syndrome 3,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:1 B:4",
      "phenotype_combined": "not specified|Familial cold autoinflammatory syndrome 3|not provided|Familial cold autoinflammatory syndrome 3;Autoinflammation-PLCG2-associated antibody deficiency-immune dysregulation",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}