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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-84177742-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=84177742&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 84177742,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "NM_178452.6",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2079C>T",
          "hgvs_p": "p.Ser693Ser",
          "transcript": "NM_178452.6",
          "protein_id": "NP_848547.4",
          "transcript_support_level": null,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2079,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000378553.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_178452.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2079C>T",
          "hgvs_p": "p.Ser693Ser",
          "transcript": "ENST00000378553.10",
          "protein_id": "ENSP00000367815.5",
          "transcript_support_level": 1,
          "aa_start": 693,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 2079,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_178452.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378553.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2181C>T",
          "hgvs_p": "p.Ser727Ser",
          "transcript": "ENST00000963697.1",
          "protein_id": "ENSP00000633756.1",
          "transcript_support_level": null,
          "aa_start": 727,
          "aa_end": null,
          "aa_length": 759,
          "cds_start": 2181,
          "cds_end": null,
          "cds_length": 2280,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963697.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2175C>T",
          "hgvs_p": "p.Ser725Ser",
          "transcript": "ENST00000963694.1",
          "protein_id": "ENSP00000633753.1",
          "transcript_support_level": null,
          "aa_start": 725,
          "aa_end": null,
          "aa_length": 757,
          "cds_start": 2175,
          "cds_end": null,
          "cds_length": 2274,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963694.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2127C>T",
          "hgvs_p": "p.Ser709Ser",
          "transcript": "ENST00000963696.1",
          "protein_id": "ENSP00000633755.1",
          "transcript_support_level": null,
          "aa_start": 709,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 2127,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963696.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2085C>T",
          "hgvs_p": "p.Ser695Ser",
          "transcript": "ENST00000897897.1",
          "protein_id": "ENSP00000567956.1",
          "transcript_support_level": null,
          "aa_start": 695,
          "aa_end": null,
          "aa_length": 727,
          "cds_start": 2085,
          "cds_end": null,
          "cds_length": 2184,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897897.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.1866C>T",
          "hgvs_p": "p.Ser622Ser",
          "transcript": "ENST00000963695.1",
          "protein_id": "ENSP00000633754.1",
          "transcript_support_level": null,
          "aa_start": 622,
          "aa_end": null,
          "aa_length": 654,
          "cds_start": 1866,
          "cds_end": null,
          "cds_length": 1965,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963695.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.1371C>T",
          "hgvs_p": "p.Ser457Ser",
          "transcript": "NM_001318756.1",
          "protein_id": "NP_001305685.1",
          "transcript_support_level": null,
          "aa_start": 457,
          "aa_end": null,
          "aa_length": 489,
          "cds_start": 1371,
          "cds_end": null,
          "cds_length": 1470,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318756.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.474C>T",
          "hgvs_p": "p.Ser158Ser",
          "transcript": "ENST00000569735.1",
          "protein_id": "ENSP00000454960.1",
          "transcript_support_level": 2,
          "aa_start": 158,
          "aa_end": null,
          "aa_length": 190,
          "cds_start": 474,
          "cds_end": null,
          "cds_length": 573,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000569735.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2220C>T",
          "hgvs_p": "p.Ser740Ser",
          "transcript": "XM_011522853.4",
          "protein_id": "XP_011521155.1",
          "transcript_support_level": null,
          "aa_start": 740,
          "aa_end": null,
          "aa_length": 772,
          "cds_start": 2220,
          "cds_end": null,
          "cds_length": 2319,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522853.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2172C>T",
          "hgvs_p": "p.Ser724Ser",
          "transcript": "XM_017022918.3",
          "protein_id": "XP_016878407.1",
          "transcript_support_level": null,
          "aa_start": 724,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 2172,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022918.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2133C>T",
          "hgvs_p": "p.Ser711Ser",
          "transcript": "XM_011522854.4",
          "protein_id": "XP_011521156.1",
          "transcript_support_level": null,
          "aa_start": 711,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 2133,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522854.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2127C>T",
          "hgvs_p": "p.Ser709Ser",
          "transcript": "XM_011522855.4",
          "protein_id": "XP_011521157.1",
          "transcript_support_level": null,
          "aa_start": 709,
          "aa_end": null,
          "aa_length": 741,
          "cds_start": 2127,
          "cds_end": null,
          "cds_length": 2226,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522855.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.2085C>T",
          "hgvs_p": "p.Ser695Ser",
          "transcript": "XM_006721129.4",
          "protein_id": "XP_006721192.1",
          "transcript_support_level": null,
          "aa_start": 695,
          "aa_end": null,
          "aa_length": 727,
          "cds_start": 2085,
          "cds_end": null,
          "cds_length": 2184,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006721129.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.1959C>T",
          "hgvs_p": "p.Ser653Ser",
          "transcript": "XM_017022919.2",
          "protein_id": "XP_016878408.1",
          "transcript_support_level": null,
          "aa_start": 653,
          "aa_end": null,
          "aa_length": 685,
          "cds_start": 1959,
          "cds_end": null,
          "cds_length": 2058,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022919.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.1464C>T",
          "hgvs_p": "p.Ser488Ser",
          "transcript": "XM_017022920.3",
          "protein_id": "XP_016878409.1",
          "transcript_support_level": null,
          "aa_start": 488,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 1464,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017022920.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "c.158-648C>T",
          "hgvs_p": null,
          "transcript": "ENST00000564928.1",
          "protein_id": "ENSP00000457899.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 57,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 174,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000564928.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "n.551C>T",
          "hgvs_p": null,
          "transcript": "ENST00000562024.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000562024.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "n.1756C>T",
          "hgvs_p": null,
          "transcript": "ENST00000563818.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000563818.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DNAAF1",
          "gene_hgnc_id": 30539,
          "hgvs_c": "n.4532C>T",
          "hgvs_p": null,
          "transcript": "ENST00000570298.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000570298.5"
        },
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      "computational_score_selected": -0.7400000095367432,
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      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "bayesdelnoaf_score": -0.74,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.352,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6,BP7",
      "acmg_by_gene": [
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          "verdict": "Likely_benign",
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      "clinvar_disease": "DNAAF1-related disorder,Primary ciliary dyskinesia,Primary ciliary dyskinesia 13",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:2",
      "phenotype_combined": "Primary ciliary dyskinesia|Primary ciliary dyskinesia 13|DNAAF1-related disorder",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
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  ],
  "message": null
}