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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-31350241-ACTT-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=31350241&ref=ACTT&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 31350241,
      "ref": "ACTT",
      "alt": "A",
      "effect": "conservative_inframe_deletion",
      "transcript": "ENST00000358273.9",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 50,
          "exon_rank_end": null,
          "exon_count": 58,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7387_7389delCTT",
          "hgvs_p": "p.Leu2463del",
          "transcript": "NM_001042492.3",
          "protein_id": "NP_001035957.1",
          "transcript_support_level": null,
          "aa_start": 2463,
          "aa_end": null,
          "aa_length": 2839,
          "cds_start": 7387,
          "cds_end": null,
          "cds_length": 8520,
          "cdna_start": 7720,
          "cdna_end": null,
          "cdna_length": 12373,
          "mane_select": "ENST00000358273.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 50,
          "exon_rank_end": null,
          "exon_count": 58,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7387_7389delCTT",
          "hgvs_p": "p.Leu2463del",
          "transcript": "ENST00000358273.9",
          "protein_id": "ENSP00000351015.4",
          "transcript_support_level": 1,
          "aa_start": 2463,
          "aa_end": null,
          "aa_length": 2839,
          "cds_start": 7387,
          "cds_end": null,
          "cds_length": 8520,
          "cdna_start": 7720,
          "cdna_end": null,
          "cdna_length": 12373,
          "mane_select": "NM_001042492.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 49,
          "exon_rank_end": null,
          "exon_count": 57,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7324_7326delCTT",
          "hgvs_p": "p.Leu2442del",
          "transcript": "ENST00000356175.7",
          "protein_id": "ENSP00000348498.3",
          "transcript_support_level": 1,
          "aa_start": 2442,
          "aa_end": null,
          "aa_length": 2818,
          "cds_start": 7324,
          "cds_end": null,
          "cds_length": 8457,
          "cdna_start": 7707,
          "cdna_end": null,
          "cdna_length": 12362,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 50,
          "exon_rank_end": null,
          "exon_count": 58,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "n.*2552_*2554delCTT",
          "hgvs_p": null,
          "transcript": "ENST00000579081.6",
          "protein_id": "ENSP00000462408.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 8788,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 50,
          "exon_rank_end": null,
          "exon_count": 58,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "n.*2552_*2554delCTT",
          "hgvs_p": null,
          "transcript": "ENST00000579081.6",
          "protein_id": "ENSP00000462408.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 8788,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 51,
          "exon_rank_end": null,
          "exon_count": 59,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7417_7419delCTT",
          "hgvs_p": "p.Leu2473del",
          "transcript": "ENST00000691014.1",
          "protein_id": "ENSP00000510595.1",
          "transcript_support_level": null,
          "aa_start": 2473,
          "aa_end": null,
          "aa_length": 2849,
          "cds_start": 7417,
          "cds_end": null,
          "cds_length": 8550,
          "cdna_start": 7750,
          "cdna_end": null,
          "cdna_length": 12415,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 49,
          "exon_rank_end": null,
          "exon_count": 57,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7324_7326delCTT",
          "hgvs_p": "p.Leu2442del",
          "transcript": "NM_000267.4",
          "protein_id": "NP_000258.1",
          "transcript_support_level": null,
          "aa_start": 2442,
          "aa_end": null,
          "aa_length": 2818,
          "cds_start": 7324,
          "cds_end": null,
          "cds_length": 8457,
          "cdna_start": 7657,
          "cdna_end": null,
          "cdna_length": 12310,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 50,
          "exon_rank_end": null,
          "exon_count": 59,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.7369_7371delCTT",
          "hgvs_p": "p.Leu2457del",
          "transcript": "ENST00000696138.1",
          "protein_id": "ENSP00000512431.1",
          "transcript_support_level": null,
          "aa_start": 2457,
          "aa_end": null,
          "aa_length": 2807,
          "cds_start": 7369,
          "cds_end": null,
          "cds_length": 8424,
          "cdna_start": 7752,
          "cdna_end": null,
          "cdna_length": 12484,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 50,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.6322_6324delCTT",
          "hgvs_p": "p.Leu2108del",
          "transcript": "ENST00000456735.6",
          "protein_id": "ENSP00000389907.2",
          "transcript_support_level": 5,
          "aa_start": 2108,
          "aa_end": null,
          "aa_length": 2502,
          "cds_start": 6322,
          "cds_end": null,
          "cds_length": 7509,
          "cdna_start": 6322,
          "cdna_end": null,
          "cdna_length": 7787,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.1951_1953delCTT",
          "hgvs_p": "p.Leu651del",
          "transcript": "ENST00000684826.1",
          "protein_id": "ENSP00000509994.1",
          "transcript_support_level": null,
          "aa_start": 651,
          "aa_end": null,
          "aa_length": 1045,
          "cds_start": 1951,
          "cds_end": null,
          "cds_length": 3138,
          "cdna_start": 2660,
          "cdna_end": null,
          "cdna_length": 7344,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.1951_1953delCTT",
          "hgvs_p": "p.Leu651del",
          "transcript": "ENST00000693617.1",
          "protein_id": "ENSP00000510031.1",
          "transcript_support_level": null,
          "aa_start": 651,
          "aa_end": null,
          "aa_length": 1027,
          "cds_start": 1951,
          "cds_end": null,
          "cds_length": 3084,
          "cdna_start": 2836,
          "cdna_end": null,
          "cdna_length": 7501,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.1543_1545delCTT",
          "hgvs_p": "p.Leu515del",
          "transcript": "ENST00000687027.1",
          "protein_id": "ENSP00000508715.1",
          "transcript_support_level": null,
          "aa_start": 515,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 1543,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": 2320,
          "cdna_end": null,
          "cdna_length": 6985,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "conservative_inframe_deletion"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "c.436_438delCTT",
          "hgvs_p": "p.Leu146del",
          "transcript": "ENST00000689464.1",
          "protein_id": "ENSP00000510673.1",
          "transcript_support_level": null,
          "aa_start": 146,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 436,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": 437,
          "cdna_end": null,
          "cdna_length": 5082,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "n.769_771delCTT",
          "hgvs_p": null,
          "transcript": "ENST00000471572.6",
          "protein_id": "ENSP00000464678.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2028,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "n.529_531delCTT",
          "hgvs_p": null,
          "transcript": "ENST00000581790.5",
          "protein_id": "ENSP00000462700.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1256,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NF1",
          "gene_hgnc_id": 7765,
          "hgvs_c": "n.4032_4034delCTT",
          "hgvs_p": null,
          "transcript": "ENST00000687863.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5018,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "NF1",
      "gene_hgnc_id": 7765,
      "dbsnp": "rs786203184",
      "frequency_reference_population": 0.000015493173,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 25,
      "gnomad_exomes_af": 0.0000164223,
      "gnomad_genomes_af": 0.00000657099,
      "gnomad_exomes_ac": 24,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": 7.236,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM4_Supporting,BP6,BS2_Supporting",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 2,
          "pathogenic_score": 1,
          "criteria": [
            "PM4_Supporting",
            "BP6",
            "BS2_Supporting"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000358273.9",
          "gene_symbol": "NF1",
          "hgnc_id": 7765,
          "effects": [
            "conservative_inframe_deletion"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.7387_7389delCTT",
          "hgvs_p": "p.Leu2463del"
        }
      ],
      "clinvar_disease": " familial spinal, type 1,Café-au-lait macules with pulmonary stenosis,Cardiovascular phenotype,Hereditary cancer-predisposing syndrome,Juvenile myelomonocytic leukemia,Neurofibromatosis,Neurofibromatosis-Noonan syndrome,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:3 LB:3 B:1",
      "phenotype_combined": "Hereditary cancer-predisposing syndrome|Neurofibromatosis, type 1|not provided|Hereditary cancer-predisposing syndrome;Cardiovascular phenotype|Juvenile myelomonocytic leukemia;Neurofibromatosis, type 1;Neurofibromatosis, familial spinal;Neurofibromatosis-Noonan syndrome;Café-au-lait macules with pulmonary stenosis|not specified",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}