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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-50167716-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=50167716&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 50167716,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000262018.8",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser",
          "transcript": "NM_000023.4",
          "protein_id": "NP_000014.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": 328,
          "cdna_end": null,
          "cdna_length": 1429,
          "mane_select": "ENST00000262018.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser",
          "transcript": "ENST00000262018.8",
          "protein_id": "ENSP00000262018.3",
          "transcript_support_level": 1,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": 328,
          "cdna_end": null,
          "cdna_length": 1429,
          "mane_select": "NM_000023.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser",
          "transcript": "ENST00000344627.10",
          "protein_id": "ENSP00000345522.6",
          "transcript_support_level": 1,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": 328,
          "cdna_end": null,
          "cdna_length": 1057,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.172C>A",
          "hgvs_p": "p.Arg58Ser",
          "transcript": "ENST00000682109.1",
          "protein_id": "ENSP00000508041.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 347,
          "cds_start": 172,
          "cds_end": null,
          "cds_length": 1044,
          "cdna_start": 172,
          "cdna_end": null,
          "cdna_length": 1044,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser",
          "transcript": "ENST00000504073.2",
          "protein_id": "ENSP00000422030.2",
          "transcript_support_level": 5,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 337,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 1014,
          "cdna_start": 292,
          "cdna_end": null,
          "cdna_length": 1229,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser",
          "transcript": "NM_001135697.3",
          "protein_id": "NP_001129169.1",
          "transcript_support_level": null,
          "aa_start": 98,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 292,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": 328,
          "cdna_end": null,
          "cdna_length": 1057,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.292C>A",
          "hgvs_p": null,
          "transcript": "ENST00000512526.2",
          "protein_id": "ENSP00000426606.2",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 733,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.292C>A",
          "hgvs_p": null,
          "transcript": "ENST00000513821.5",
          "protein_id": "ENSP00000426571.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1090,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.2C>A",
          "hgvs_p": null,
          "transcript": "ENST00000683226.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1922,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.292C>A",
          "hgvs_p": null,
          "transcript": "ENST00000683294.1",
          "protein_id": "ENSP00000508134.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1331,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.328C>A",
          "hgvs_p": null,
          "transcript": "NR_135553.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1220,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.157+229C>A",
          "hgvs_p": null,
          "transcript": "ENST00000502555.5",
          "protein_id": "ENSP00000422817.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1349,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.38-231C>A",
          "hgvs_p": null,
          "transcript": "ENST00000511303.6",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 672,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.104-231C>A",
          "hgvs_p": null,
          "transcript": "ENST00000513942.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 734,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SGCA",
          "gene_hgnc_id": 10805,
          "hgvs_c": "n.*18+229C>A",
          "hgvs_p": null,
          "transcript": "ENST00000514934.1",
          "protein_id": "ENSP00000423168.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 542,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000253730",
          "gene_hgnc_id": null,
          "hgvs_c": "n.544-9093G>T",
          "hgvs_p": null,
          "transcript": "ENST00000751801.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 835,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SGCA",
      "gene_hgnc_id": 10805,
      "dbsnp": "rs138945081",
      "frequency_reference_population": 0.000002055414,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205541,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8389497995376587,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.561,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2407,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.15,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 0.334,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 9,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP3,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 9,
          "benign_score": 0,
          "pathogenic_score": 9,
          "criteria": [
            "PM1",
            "PM2",
            "PM5",
            "PP3",
            "PP5_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000262018.8",
          "gene_symbol": "SGCA",
          "hgnc_id": 10805,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.292C>A",
          "hgvs_p": "p.Arg98Ser"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000751801.1",
          "gene_symbol": "ENSG00000253730",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.544-9093G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Autosomal recessive limb-girdle muscular dystrophy",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Autosomal recessive limb-girdle muscular dystrophy",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}