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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-7224040-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=7224040&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 7224040,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001270447.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "NM_000018.4",
          "protein_id": "NP_000009.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000356839.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000018.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000356839.10",
          "protein_id": "ENSP00000349297.5",
          "transcript_support_level": 1,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000018.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000356839.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1339C>T",
          "hgvs_p": "p.Arg447Trp",
          "transcript": "ENST00000350303.9",
          "protein_id": "ENSP00000344152.5",
          "transcript_support_level": 1,
          "aa_start": 447,
          "aa_end": null,
          "aa_length": 633,
          "cds_start": 1339,
          "cds_end": null,
          "cds_length": 1902,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000350303.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1474C>T",
          "hgvs_p": "p.Arg492Trp",
          "transcript": "NM_001270447.2",
          "protein_id": "NP_001257376.1",
          "transcript_support_level": null,
          "aa_start": 492,
          "aa_end": null,
          "aa_length": 678,
          "cds_start": 1474,
          "cds_end": null,
          "cds_length": 2037,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001270447.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1474C>T",
          "hgvs_p": "p.Arg492Trp",
          "transcript": "ENST00000543245.6",
          "protein_id": "ENSP00000438689.2",
          "transcript_support_level": 2,
          "aa_start": 492,
          "aa_end": null,
          "aa_length": 678,
          "cds_start": 1474,
          "cds_end": null,
          "cds_length": 2037,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543245.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1447C>T",
          "hgvs_p": "p.Arg483Trp",
          "transcript": "ENST00000945302.1",
          "protein_id": "ENSP00000615361.1",
          "transcript_support_level": null,
          "aa_start": 483,
          "aa_end": null,
          "aa_length": 669,
          "cds_start": 1447,
          "cds_end": null,
          "cds_length": 2010,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945302.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1435C>T",
          "hgvs_p": "p.Arg479Trp",
          "transcript": "ENST00000883761.1",
          "protein_id": "ENSP00000553820.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 665,
          "cds_start": 1435,
          "cds_end": null,
          "cds_length": 1998,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883761.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1429C>T",
          "hgvs_p": "p.Arg477Trp",
          "transcript": "ENST00000945308.1",
          "protein_id": "ENSP00000615367.1",
          "transcript_support_level": null,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1429,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945308.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1423C>T",
          "hgvs_p": "p.Arg475Trp",
          "transcript": "ENST00000883770.1",
          "protein_id": "ENSP00000553829.1",
          "transcript_support_level": null,
          "aa_start": 475,
          "aa_end": null,
          "aa_length": 661,
          "cds_start": 1423,
          "cds_end": null,
          "cds_length": 1986,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883770.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000916066.1",
          "protein_id": "ENSP00000586125.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916066.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000916068.1",
          "protein_id": "ENSP00000586127.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916068.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1402C>T",
          "hgvs_p": "p.Arg468Trp",
          "transcript": "ENST00000883764.1",
          "protein_id": "ENSP00000553823.1",
          "transcript_support_level": null,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 654,
          "cds_start": 1402,
          "cds_end": null,
          "cds_length": 1965,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883764.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000883757.1",
          "protein_id": "ENSP00000553816.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883757.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1396C>T",
          "hgvs_p": "p.Arg466Trp",
          "transcript": "ENST00000945291.1",
          "protein_id": "ENSP00000615350.1",
          "transcript_support_level": null,
          "aa_start": 466,
          "aa_end": null,
          "aa_length": 652,
          "cds_start": 1396,
          "cds_end": null,
          "cds_length": 1959,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000945291.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
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          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000945298.1",
          "protein_id": "ENSP00000615357.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 651,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1956,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945298.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1390C>T",
          "hgvs_p": "p.Arg464Trp",
          "transcript": "ENST00000945297.1",
          "protein_id": "ENSP00000615356.1",
          "transcript_support_level": null,
          "aa_start": 464,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 1390,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945297.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000883762.1",
          "protein_id": "ENSP00000553821.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1405,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000883762.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1405C>T",
          "hgvs_p": "p.Arg469Trp",
          "transcript": "ENST00000883763.1",
          "protein_id": "ENSP00000553822.1",
          "transcript_support_level": null,
          "aa_start": 469,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1405,
          "cds_end": null,
          "cds_length": 1944,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883763.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1381C>T",
          "hgvs_p": "p.Arg461Trp",
          "transcript": "ENST00000945292.1",
          "protein_id": "ENSP00000615351.1",
          "transcript_support_level": null,
          "aa_start": 461,
          "aa_end": null,
          "aa_length": 647,
          "cds_start": 1381,
          "cds_end": null,
          "cds_length": 1944,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945292.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACADVL",
          "gene_hgnc_id": 92,
          "hgvs_c": "c.1378C>T",
          "hgvs_p": "p.Arg460Trp",
          "transcript": "ENST00000945303.1",
          "protein_id": "ENSP00000615362.1",
          "transcript_support_level": null,
          "aa_start": 460,
          "aa_end": null,
          "aa_length": 646,
          "cds_start": 1378,
          "cds_end": null,
          "cds_length": 1941,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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      ],
      "gene_symbol": "ACADVL",
      "gene_hgnc_id": 92,
      "dbsnp": "rs113994170",
      "frequency_reference_population": 0.00000867408,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 14,
      "gnomad_exomes_af": 0.00000820864,
      "gnomad_genomes_af": 0.0000131467,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9822096824645996,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.947,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.6925,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.54,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 1.829,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 9,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2_Supporting,PM1,PM3,PS3_Supporting,PP4_Moderate,PP3",
      "acmg_by_gene": [
        {
          "score": 9,
          "benign_score": 0,
          "pathogenic_score": 9,
          "criteria": [
            "PM2_Supporting",
            "PM1",
            "PM3",
            "PS3_Supporting",
            "PP4_Moderate",
            "PP3"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001270447.2",
          "gene_symbol": "ACADVL",
          "hgnc_id": 92,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1474C>T",
          "hgvs_p": "p.Arg492Trp"
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      ],
      "clinvar_disease": "Very long chain acyl-CoA dehydrogenase deficiency",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "P:7 LP:2",
      "phenotype_combined": "Very long chain acyl-CoA dehydrogenase deficiency",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}