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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-12647289-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=12647289&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 12647289,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001440570.1",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2867T>G",
          "hgvs_p": "p.Leu956Arg",
          "transcript": "NM_000528.4",
          "protein_id": "NP_000519.2",
          "transcript_support_level": null,
          "aa_start": 956,
          "aa_end": null,
          "aa_length": 1011,
          "cds_start": 2867,
          "cds_end": null,
          "cds_length": 3036,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000456935.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000528.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2867T>G",
          "hgvs_p": "p.Leu956Arg",
          "transcript": "ENST00000456935.7",
          "protein_id": "ENSP00000395473.2",
          "transcript_support_level": 1,
          "aa_start": 956,
          "aa_end": null,
          "aa_length": 1011,
          "cds_start": 2867,
          "cds_end": null,
          "cds_length": 3036,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000528.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000456935.7"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2864T>G",
          "hgvs_p": "p.Leu955Arg",
          "transcript": "ENST00000221363.9",
          "protein_id": "ENSP00000221363.4",
          "transcript_support_level": 1,
          "aa_start": 955,
          "aa_end": null,
          "aa_length": 1010,
          "cds_start": 2864,
          "cds_end": null,
          "cds_length": 3033,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000221363.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000269242",
          "gene_hgnc_id": null,
          "hgvs_c": "n.425T>G",
          "hgvs_p": null,
          "transcript": "ENST00000597692.1",
          "protein_id": "ENSP00000470240.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000597692.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2915T>G",
          "hgvs_p": "p.Leu972Arg",
          "transcript": "ENST00000964003.1",
          "protein_id": "ENSP00000634062.1",
          "transcript_support_level": null,
          "aa_start": 972,
          "aa_end": null,
          "aa_length": 1027,
          "cds_start": 2915,
          "cds_end": null,
          "cds_length": 3084,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964003.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2870T>G",
          "hgvs_p": "p.Leu957Arg",
          "transcript": "NM_001440570.1",
          "protein_id": "NP_001427499.1",
          "transcript_support_level": null,
          "aa_start": 957,
          "aa_end": null,
          "aa_length": 1012,
          "cds_start": 2870,
          "cds_end": null,
          "cds_length": 3039,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440570.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2870T>G",
          "hgvs_p": "p.Leu957Arg",
          "transcript": "ENST00000858849.1",
          "protein_id": "ENSP00000528908.1",
          "transcript_support_level": null,
          "aa_start": 957,
          "aa_end": null,
          "aa_length": 1012,
          "cds_start": 2870,
          "cds_end": null,
          "cds_length": 3039,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858849.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2864T>G",
          "hgvs_p": "p.Leu955Arg",
          "transcript": "NM_001173498.2",
          "protein_id": "NP_001166969.1",
          "transcript_support_level": null,
          "aa_start": 955,
          "aa_end": null,
          "aa_length": 1010,
          "cds_start": 2864,
          "cds_end": null,
          "cds_length": 3033,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001173498.2"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2861T>G",
          "hgvs_p": "p.Leu954Arg",
          "transcript": "ENST00000963998.1",
          "protein_id": "ENSP00000634057.1",
          "transcript_support_level": null,
          "aa_start": 954,
          "aa_end": null,
          "aa_length": 1009,
          "cds_start": 2861,
          "cds_end": null,
          "cds_length": 3030,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963998.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2855T>G",
          "hgvs_p": "p.Leu952Arg",
          "transcript": "ENST00000963997.1",
          "protein_id": "ENSP00000634056.1",
          "transcript_support_level": null,
          "aa_start": 952,
          "aa_end": null,
          "aa_length": 1007,
          "cds_start": 2855,
          "cds_end": null,
          "cds_length": 3024,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963997.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2834T>G",
          "hgvs_p": "p.Leu945Arg",
          "transcript": "ENST00000858857.1",
          "protein_id": "ENSP00000528916.1",
          "transcript_support_level": null,
          "aa_start": 945,
          "aa_end": null,
          "aa_length": 1000,
          "cds_start": 2834,
          "cds_end": null,
          "cds_length": 3003,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858857.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2816T>G",
          "hgvs_p": "p.Leu939Arg",
          "transcript": "ENST00000935810.1",
          "protein_id": "ENSP00000605869.1",
          "transcript_support_level": null,
          "aa_start": 939,
          "aa_end": null,
          "aa_length": 994,
          "cds_start": 2816,
          "cds_end": null,
          "cds_length": 2985,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935810.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2801T>G",
          "hgvs_p": "p.Leu934Arg",
          "transcript": "ENST00000858853.1",
          "protein_id": "ENSP00000528912.1",
          "transcript_support_level": null,
          "aa_start": 934,
          "aa_end": null,
          "aa_length": 989,
          "cds_start": 2801,
          "cds_end": null,
          "cds_length": 2970,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858853.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2798T>G",
          "hgvs_p": "p.Leu933Arg",
          "transcript": "ENST00000858851.1",
          "protein_id": "ENSP00000528910.1",
          "transcript_support_level": null,
          "aa_start": 933,
          "aa_end": null,
          "aa_length": 988,
          "cds_start": 2798,
          "cds_end": null,
          "cds_length": 2967,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858851.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2798T>G",
          "hgvs_p": "p.Leu933Arg",
          "transcript": "ENST00000963999.1",
          "protein_id": "ENSP00000634058.1",
          "transcript_support_level": null,
          "aa_start": 933,
          "aa_end": null,
          "aa_length": 988,
          "cds_start": 2798,
          "cds_end": null,
          "cds_length": 2967,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963999.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2786T>G",
          "hgvs_p": "p.Leu929Arg",
          "transcript": "ENST00000858852.1",
          "protein_id": "ENSP00000528911.1",
          "transcript_support_level": null,
          "aa_start": 929,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 2786,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858852.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2765T>G",
          "hgvs_p": "p.Leu922Arg",
          "transcript": "ENST00000935811.1",
          "protein_id": "ENSP00000605870.1",
          "transcript_support_level": null,
          "aa_start": 922,
          "aa_end": null,
          "aa_length": 977,
          "cds_start": 2765,
          "cds_end": null,
          "cds_length": 2934,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000935811.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2753T>G",
          "hgvs_p": "p.Leu918Arg",
          "transcript": "ENST00000935809.1",
          "protein_id": "ENSP00000605868.1",
          "transcript_support_level": null,
          "aa_start": 918,
          "aa_end": null,
          "aa_length": 973,
          "cds_start": 2753,
          "cds_end": null,
          "cds_length": 2922,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000935809.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2750T>G",
          "hgvs_p": "p.Leu917Arg",
          "transcript": "ENST00000858850.1",
          "protein_id": "ENSP00000528909.1",
          "transcript_support_level": null,
          "aa_start": 917,
          "aa_end": null,
          "aa_length": 972,
          "cds_start": 2750,
          "cds_end": null,
          "cds_length": 2919,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000858850.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAN2B1",
          "gene_hgnc_id": 6826,
          "hgvs_c": "c.2714T>G",
          "hgvs_p": "p.Leu905Arg",
          "transcript": "ENST00000964001.1",
          "protein_id": "ENSP00000634060.1",
          "transcript_support_level": null,
          "aa_start": 905,
          "aa_end": null,
          "aa_length": 960,
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      "gene_symbol": "MAN2B1",
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      "dbsnp": "rs768233248",
      "frequency_reference_population": 0.000012312912,
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      "gnomad_exomes_af": 0.0000123129,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 18,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9871559143066406,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.933,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.7465,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.53,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.246,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001440570.1",
          "gene_symbol": "MAN2B1",
          "hgnc_id": 6826,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2870T>G",
          "hgvs_p": "p.Leu957Arg"
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        {
          "score": 5,
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            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000597692.1",
          "gene_symbol": "ENSG00000269242",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.425T>G",
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      ],
      "clinvar_disease": "Deficiency of alpha-mannosidase",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "LP:4 US:2",
      "phenotype_combined": "Deficiency of alpha-mannosidase",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}