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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-48231685-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=48231685&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 48231685,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001184900.3",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "NM_001184900.3",
          "protein_id": "NP_001171829.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000651546.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001184900.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000651546.1",
          "protein_id": "ENSP00000499211.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001184900.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000651546.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000391898.7",
          "protein_id": "ENSP00000375767.3",
          "transcript_support_level": 1,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000391898.7"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.367A>G",
          "hgvs_p": "p.Ile123Val",
          "transcript": "ENST00000520153.5",
          "protein_id": "ENSP00000428736.1",
          "transcript_support_level": 1,
          "aa_start": 123,
          "aa_end": null,
          "aa_length": 487,
          "cds_start": 367,
          "cds_end": null,
          "cds_length": 1464,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000520153.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000520753.5",
          "protein_id": "ENSP00000429839.1",
          "transcript_support_level": 1,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000520753.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000518622.5",
          "protein_id": "ENSP00000430057.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000518622.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000518979.5",
          "protein_id": "ENSP00000430495.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000518979.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000521415.5",
          "protein_id": "ENSP00000428862.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000521415.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*233A>G",
          "hgvs_p": null,
          "transcript": "ENST00000522773.5",
          "protein_id": "ENSP00000429362.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522773.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000518622.5",
          "protein_id": "ENSP00000430057.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000518622.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000518979.5",
          "protein_id": "ENSP00000430495.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000518979.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*77A>G",
          "hgvs_p": null,
          "transcript": "ENST00000521415.5",
          "protein_id": "ENSP00000428862.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000521415.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "n.*233A>G",
          "hgvs_p": null,
          "transcript": "ENST00000522773.5",
          "protein_id": "ENSP00000429362.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000522773.5"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "NM_001351782.2",
          "protein_id": "NP_001338711.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001351782.2"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000519940.6",
          "protein_id": "ENSP00000428883.1",
          "transcript_support_level": 5,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000519940.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000877775.1",
          "protein_id": "ENSP00000547834.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877775.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000877776.1",
          "protein_id": "ENSP00000547835.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000877776.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000930774.1",
          "protein_id": "ENSP00000600833.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930774.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000946860.1",
          "protein_id": "ENSP00000616919.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946860.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CARD8",
          "gene_hgnc_id": 17057,
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val",
          "transcript": "ENST00000946861.1",
          "protein_id": "ENSP00000616920.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946861.1"
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      "hom_count_reference_population": 726,
      "allele_count_reference_population": 14389,
      "gnomad_exomes_af": 0.0057108,
      "gnomad_genomes_af": 0.0399067,
      "gnomad_exomes_ac": 8314,
      "gnomad_genomes_ac": 6075,
      "gnomad_exomes_homalt": 335,
      "gnomad_genomes_homalt": 391,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0034451186656951904,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.014,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0906,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.71,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.148,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001184900.3",
          "gene_symbol": "CARD8",
          "hgnc_id": 17057,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown",
          "hgvs_c": "c.517A>G",
          "hgvs_p": "p.Ile173Val"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}