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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-151872444-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=151872444&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 151872444,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_000726.5",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.571A>T",
          "hgvs_p": "p.Thr191Ser",
          "transcript": "NM_000726.5",
          "protein_id": "NP_000717.2",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000539935.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000726.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.571A>T",
          "hgvs_p": "p.Thr191Ser",
          "transcript": "ENST00000539935.7",
          "protein_id": "ENSP00000438949.1",
          "transcript_support_level": 1,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 520,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1563,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000726.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000539935.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.469A>T",
          "hgvs_p": "p.Thr157Ser",
          "transcript": "ENST00000534999.7",
          "protein_id": "ENSP00000443893.1",
          "transcript_support_level": 1,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000534999.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.571A>T",
          "hgvs_p": "p.Thr191Ser",
          "transcript": "ENST00000201943.10",
          "protein_id": "ENSP00000201943.5",
          "transcript_support_level": 1,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000201943.10"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.469A>T",
          "hgvs_p": "p.Thr157Ser",
          "transcript": "ENST00000636947.1",
          "protein_id": "ENSP00000490337.1",
          "transcript_support_level": 1,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 307,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 924,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636947.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000283228",
          "gene_hgnc_id": null,
          "hgvs_c": "n.469A>T",
          "hgvs_p": null,
          "transcript": "ENST00000637559.1",
          "protein_id": "ENSP00000489697.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000637559.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.517A>T",
          "hgvs_p": "p.Thr173Ser",
          "transcript": "NM_001005746.4",
          "protein_id": "NP_001005746.1",
          "transcript_support_level": null,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001005746.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.517A>T",
          "hgvs_p": "p.Thr173Ser",
          "transcript": "ENST00000638005.1",
          "protein_id": "ENSP00000489677.1",
          "transcript_support_level": 2,
          "aa_start": 173,
          "aa_end": null,
          "aa_length": 502,
          "cds_start": 517,
          "cds_end": null,
          "cds_length": 1509,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000638005.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.481A>T",
          "hgvs_p": "p.Thr161Ser",
          "transcript": "ENST00000637762.1",
          "protein_id": "ENSP00000489876.1",
          "transcript_support_level": 5,
          "aa_start": 161,
          "aa_end": null,
          "aa_length": 490,
          "cds_start": 481,
          "cds_end": null,
          "cds_length": 1473,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637762.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.469A>T",
          "hgvs_p": "p.Thr157Ser",
          "transcript": "NM_001005747.4",
          "protein_id": "NP_001005747.1",
          "transcript_support_level": null,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 486,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 1461,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001005747.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.448A>T",
          "hgvs_p": "p.Thr150Ser",
          "transcript": "ENST00000427385.6",
          "protein_id": "ENSP00000410978.2",
          "transcript_support_level": 5,
          "aa_start": 150,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 448,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000427385.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.430A>T",
          "hgvs_p": "p.Thr144Ser",
          "transcript": "NM_001320722.3",
          "protein_id": "NP_001307651.1",
          "transcript_support_level": null,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001320722.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.430A>T",
          "hgvs_p": "p.Thr144Ser",
          "transcript": "NM_001330118.1",
          "protein_id": "NP_001317047.1",
          "transcript_support_level": null,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330118.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.430A>T",
          "hgvs_p": "p.Thr144Ser",
          "transcript": "ENST00000439467.6",
          "protein_id": "ENSP00000390161.2",
          "transcript_support_level": 5,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000439467.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.430A>T",
          "hgvs_p": "p.Thr144Ser",
          "transcript": "ENST00000636598.1",
          "protein_id": "ENSP00000490247.1",
          "transcript_support_level": 5,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636598.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.430A>T",
          "hgvs_p": "p.Thr144Ser",
          "transcript": "ENST00000637217.1",
          "protein_id": "ENSP00000490250.1",
          "transcript_support_level": 5,
          "aa_start": 144,
          "aa_end": null,
          "aa_length": 473,
          "cds_start": 430,
          "cds_end": null,
          "cds_length": 1422,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637217.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.469A>T",
          "hgvs_p": "p.Thr157Ser",
          "transcript": "ENST00000360283.11",
          "protein_id": "ENSP00000353425.8",
          "transcript_support_level": 5,
          "aa_start": 157,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 469,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360283.11"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.394A>T",
          "hgvs_p": "p.Thr132Ser",
          "transcript": "ENST00000636442.1",
          "protein_id": "ENSP00000489779.1",
          "transcript_support_level": 5,
          "aa_start": 132,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 394,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636442.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.571A>T",
          "hgvs_p": "p.Thr191Ser",
          "transcript": "NM_001145798.2",
          "protein_id": "NP_001139270.1",
          "transcript_support_level": null,
          "aa_start": 191,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 571,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145798.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CACNB4",
          "gene_hgnc_id": 1404,
          "hgvs_c": "c.346A>T",
          "hgvs_p": "p.Thr116Ser",
          "transcript": "ENST00000636350.1",
          "protein_id": "ENSP00000489621.1",
          "transcript_support_level": 5,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 346,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636350.1"
        },
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        {
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          "gene_symbol": "CACNB4",
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          "hgvs_c": "n.419+3982A>T",
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          "transcript": "ENST00000637550.1",
          "protein_id": "ENSP00000489943.1",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000637550.1"
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      ],
      "gene_symbol": "CACNB4",
      "gene_hgnc_id": 1404,
      "dbsnp": "rs746415223",
      "frequency_reference_population": 0.000011220029,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 18,
      "gnomad_exomes_af": 0.0000110186,
      "gnomad_genomes_af": 0.0000131418,
      "gnomad_exomes_ac": 16,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.20594221353530884,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.45,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0705,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.26,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.92,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000726.5",
          "gene_symbol": "CACNB4",
          "hgnc_id": 1404,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.571A>T",
          "hgvs_p": "p.Thr191Ser"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000637559.1",
          "gene_symbol": "ENSG00000283228",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.469A>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Idiopathic generalized epilepsy,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "not provided|Idiopathic generalized epilepsy",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}