← Back to variant description

GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-46905497-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=46905497&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 46905497,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000319466.9",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "NM_139279.6",
          "protein_id": "NP_644808.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 497,
          "cdna_end": null,
          "cdna_length": 4120,
          "mane_select": "ENST00000319466.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000319466.9",
          "protein_id": "ENSP00000317271.4",
          "transcript_support_level": 1,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 497,
          "cdna_end": null,
          "cdna_length": 4120,
          "mane_select": "NM_139279.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.251T>C",
          "hgvs_p": "p.Ile84Thr",
          "transcript": "ENST00000409913.5",
          "protein_id": "ENSP00000386941.1",
          "transcript_support_level": 1,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 251,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": 352,
          "cdna_end": null,
          "cdna_length": 3975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "NM_001171506.2",
          "protein_id": "NP_001164977.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 653,
          "cdna_end": null,
          "cdna_length": 4280,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "NM_001171507.2",
          "protein_id": "NP_001164978.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 545,
          "cdna_end": null,
          "cdna_length": 4172,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "NM_001171508.2",
          "protein_id": "NP_001164979.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 697,
          "cdna_end": null,
          "cdna_length": 4324,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000409105.5",
          "protein_id": "ENSP00000386651.1",
          "transcript_support_level": 5,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 587,
          "cdna_end": null,
          "cdna_length": 4210,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000409207.5",
          "protein_id": "ENSP00000386386.1",
          "transcript_support_level": 2,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 574,
          "cdna_end": null,
          "cdna_length": 1113,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000409218.5",
          "protein_id": "ENSP00000386261.1",
          "transcript_support_level": 2,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 545,
          "cdna_end": null,
          "cdna_length": 648,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000409973.5",
          "protein_id": "ENSP00000386279.1",
          "transcript_support_level": 2,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 146,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 441,
          "cdna_start": 595,
          "cdna_end": null,
          "cdna_length": 821,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr",
          "transcript": "ENST00000412438.5",
          "protein_id": "ENSP00000402717.1",
          "transcript_support_level": 3,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": 557,
          "cdna_end": null,
          "cdna_length": 588,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.350T>C",
          "hgvs_p": "p.Ile117Thr",
          "transcript": "NM_001171511.3",
          "protein_id": "NP_001164982.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": 422,
          "cdna_end": null,
          "cdna_length": 4045,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.350T>C",
          "hgvs_p": "p.Ile117Thr",
          "transcript": "ENST00000444761.6",
          "protein_id": "ENSP00000394647.2",
          "transcript_support_level": 2,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 127,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 384,
          "cdna_start": 542,
          "cdna_end": null,
          "cdna_length": 4169,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.251T>C",
          "hgvs_p": "p.Ile84Thr",
          "transcript": "NM_001171509.3",
          "protein_id": "NP_001164980.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 251,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": 342,
          "cdna_end": null,
          "cdna_length": 3965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.251T>C",
          "hgvs_p": "p.Ile84Thr",
          "transcript": "NM_001171510.3",
          "protein_id": "NP_001164981.1",
          "transcript_support_level": null,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 251,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": 331,
          "cdna_end": null,
          "cdna_length": 3954,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.251T>C",
          "hgvs_p": "p.Ile84Thr",
          "transcript": "ENST00000409147.1",
          "protein_id": "ENSP00000387082.1",
          "transcript_support_level": 2,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 251,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": 542,
          "cdna_end": null,
          "cdna_length": 671,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "I",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.251T>C",
          "hgvs_p": "p.Ile84Thr",
          "transcript": "ENST00000409800.5",
          "protein_id": "ENSP00000387202.1",
          "transcript_support_level": 4,
          "aa_start": 84,
          "aa_end": null,
          "aa_length": 94,
          "cds_start": 251,
          "cds_end": null,
          "cds_length": 285,
          "cdna_start": 332,
          "cdna_end": null,
          "cdna_length": 3955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "n.300T>C",
          "hgvs_p": null,
          "transcript": "ENST00000470873.1",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 552,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "n.165T>C",
          "hgvs_p": null,
          "transcript": "ENST00000493804.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 807,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2",
          "gene_hgnc_id": 18451,
          "hgvs_c": "c.*73T>C",
          "hgvs_p": null,
          "transcript": "ENST00000649435.1",
          "protein_id": "ENSP00000498024.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 109,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 330,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3978,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000228925",
          "gene_hgnc_id": 58223,
          "hgvs_c": "n.80-1387A>G",
          "hgvs_p": null,
          "transcript": "ENST00000429761.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1874,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "LINC01118",
          "gene_hgnc_id": 49261,
          "hgvs_c": "n.718-7679A>G",
          "hgvs_p": null,
          "transcript": "ENST00000759083.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 773,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MCFD2-AS1",
          "gene_hgnc_id": 58223,
          "hgvs_c": "n.41-1387A>G",
          "hgvs_p": null,
          "transcript": "NR_199007.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1835,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "MCFD2",
      "gene_hgnc_id": 18451,
      "dbsnp": "rs137852914",
      "frequency_reference_population": 0.0000027379408,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 4,
      "gnomad_exomes_af": 0.00000273794,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 4,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9379702806472778,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.899,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9771,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.45,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.68,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000319466.9",
          "gene_symbol": "MCFD2",
          "hgnc_id": 18451,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.407T>C",
          "hgvs_p": "p.Ile136Thr"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000429761.2",
          "gene_symbol": "ENSG00000228925",
          "hgnc_id": 58223,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.80-1387A>G",
          "hgvs_p": null
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NR_199007.1",
          "gene_symbol": "MCFD2-AS1",
          "hgnc_id": 58223,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.41-1387A>G",
          "hgvs_p": null
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000759083.1",
          "gene_symbol": "LINC01118",
          "hgnc_id": 49261,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.718-7679A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " 2, combined deficiency of,Factor 5 and Factor VIII",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Factor 5 and Factor VIII, combined deficiency of, 2",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}