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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 2-98395997-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=2&pos=98395997&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "2",
      "pos": 98395997,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000272602.7",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.827A>G",
          "hgvs_p": "p.Asn276Ser",
          "transcript": "NM_001298.3",
          "protein_id": "NP_001289.1",
          "transcript_support_level": null,
          "aa_start": 276,
          "aa_end": null,
          "aa_length": 694,
          "cds_start": 827,
          "cds_end": null,
          "cds_length": 2085,
          "cdna_start": 943,
          "cdna_end": null,
          "cdna_length": 3547,
          "mane_select": "ENST00000272602.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.827A>G",
          "hgvs_p": "p.Asn276Ser",
          "transcript": "ENST00000272602.7",
          "protein_id": "ENSP00000272602.2",
          "transcript_support_level": 1,
          "aa_start": 276,
          "aa_end": null,
          "aa_length": 694,
          "cds_start": 827,
          "cds_end": null,
          "cds_length": 2085,
          "cdna_start": 943,
          "cdna_end": null,
          "cdna_length": 3547,
          "mane_select": "NM_001298.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.773A>G",
          "hgvs_p": "p.Asn258Ser",
          "transcript": "ENST00000436404.6",
          "protein_id": "ENSP00000410070.2",
          "transcript_support_level": 1,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 676,
          "cds_start": 773,
          "cds_end": null,
          "cds_length": 2031,
          "cdna_start": 1157,
          "cdna_end": null,
          "cdna_length": 2504,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.773A>G",
          "hgvs_p": "p.Asn258Ser",
          "transcript": "NM_001079878.2",
          "protein_id": "NP_001073347.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 676,
          "cds_start": 773,
          "cds_end": null,
          "cds_length": 2031,
          "cdna_start": 889,
          "cdna_end": null,
          "cdna_length": 3493,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.992A>G",
          "hgvs_p": "p.Asn331Ser",
          "transcript": "XM_011510554.3",
          "protein_id": "XP_011508856.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": 1191,
          "cdna_end": null,
          "cdna_length": 3795,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.992A>G",
          "hgvs_p": "p.Asn331Ser",
          "transcript": "XM_047443222.1",
          "protein_id": "XP_047299178.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": 1108,
          "cdna_end": null,
          "cdna_length": 3712,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "c.938A>G",
          "hgvs_p": "p.Asn313Ser",
          "transcript": "XM_006712243.3",
          "protein_id": "XP_006712306.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": 1054,
          "cdna_end": null,
          "cdna_length": 3658,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CNGA3",
          "gene_hgnc_id": 2150,
          "hgvs_c": "n.980A>G",
          "hgvs_p": null,
          "transcript": "ENST00000409937.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2615,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "CNGA3",
      "gene_hgnc_id": 2150,
      "dbsnp": "rs199474697",
      "frequency_reference_population": 0.0000013680976,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.0000013681,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9151265025138855,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.821,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1807,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.06,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 8.947,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 15,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PP2,PP3_Moderate,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 15,
          "benign_score": 0,
          "pathogenic_score": 15,
          "criteria": [
            "PM1",
            "PM2",
            "PP2",
            "PP3_Moderate",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000272602.7",
          "gene_symbol": "CNGA3",
          "hgnc_id": 2150,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.827A>G",
          "hgvs_p": "p.Asn276Ser"
        }
      ],
      "clinvar_disease": "Achromatopsia,Achromatopsia 2,not provided",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LP:3 O:1",
      "phenotype_combined": "Achromatopsia 2|not provided|Achromatopsia",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}