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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-44406229-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=44406229&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 44406229,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000316673.9",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "NM_175914.5",
          "protein_id": "NP_787110.2",
          "transcript_support_level": null,
          "aa_start": 74,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 221,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": 327,
          "cdna_end": null,
          "cdna_length": 4645,
          "mane_select": "ENST00000316673.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "ENST00000316673.9",
          "protein_id": "ENSP00000315180.4",
          "transcript_support_level": 1,
          "aa_start": 74,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 221,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": 327,
          "cdna_end": null,
          "cdna_length": 4645,
          "mane_select": "NM_175914.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "ENST00000316099.10",
          "protein_id": "ENSP00000312987.3",
          "transcript_support_level": 1,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": 421,
          "cdna_end": null,
          "cdna_length": 4739,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "ENST00000415691.2",
          "protein_id": "ENSP00000412111.1",
          "transcript_support_level": 1,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 359,
          "cdna_end": null,
          "cdna_length": 2302,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "ENST00000457232.5",
          "protein_id": "ENSP00000396216.1",
          "transcript_support_level": 1,
          "aa_start": 74,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 221,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": 225,
          "cdna_end": null,
          "cdna_length": 1339,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "ENST00000443598.6",
          "protein_id": "ENSP00000410911.2",
          "transcript_support_level": 1,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 376,
          "cdna_end": null,
          "cdna_length": 1603,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "ENST00000609795.5",
          "protein_id": "ENSP00000476609.1",
          "transcript_support_level": 1,
          "aa_start": 74,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 221,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": 225,
          "cdna_end": null,
          "cdna_length": 1192,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.212G>A",
          "hgvs_p": "p.Cys71Tyr",
          "transcript": "ENST00000609262.5",
          "protein_id": "ENSP00000476310.1",
          "transcript_support_level": 1,
          "aa_start": 71,
          "aa_end": null,
          "aa_length": 102,
          "cds_start": 212,
          "cds_end": null,
          "cds_length": 309,
          "cdna_start": 443,
          "cdna_end": null,
          "cdna_length": 540,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "n.*54G>A",
          "hgvs_p": null,
          "transcript": "ENST00000372920.1",
          "protein_id": "ENSP00000362011.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3340,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "n.*54G>A",
          "hgvs_p": null,
          "transcript": "ENST00000372920.1",
          "protein_id": "ENSP00000362011.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3340,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "NM_000457.6",
          "protein_id": "NP_000448.3",
          "transcript_support_level": null,
          "aa_start": 96,
          "aa_end": null,
          "aa_length": 474,
          "cds_start": 287,
          "cds_end": null,
          "cds_length": 1425,
          "cdna_start": 421,
          "cdna_end": null,
          "cdna_length": 4739,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.266G>A",
          "hgvs_p": "p.Cys89Tyr",
          "transcript": "NM_001258355.2",
          "protein_id": "NP_001245284.1",
          "transcript_support_level": null,
          "aa_start": 89,
          "aa_end": null,
          "aa_length": 467,
          "cds_start": 266,
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          "cdna_start": 512,
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          "cdna_length": 6581,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "NM_178849.3",
          "protein_id": "NP_849180.1",
          "transcript_support_level": null,
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          "aa_end": null,
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          "cds_start": 287,
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          "cdna_start": 421,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.212G>A",
          "hgvs_p": "p.Cys71Tyr",
          "transcript": "NM_001287183.2",
          "protein_id": "NP_001274112.1",
          "transcript_support_level": null,
          "aa_start": 71,
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          "aa_length": 449,
          "cds_start": 212,
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          "cds_length": 1350,
          "cdna_start": 549,
          "cdna_end": null,
          "cdna_length": 4867,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "gene_symbol": "HNF4A",
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          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "NM_001030003.3",
          "protein_id": "NP_001025174.1",
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
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          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.212G>A",
          "hgvs_p": "p.Cys71Tyr",
          "transcript": "NM_001287182.2",
          "protein_id": "NP_001274111.1",
          "transcript_support_level": null,
          "aa_start": 71,
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          "cds_start": 212,
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          "cds_length": 1320,
          "cdna_start": 549,
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          "cdna_length": 6588,
          "mane_select": null,
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        },
        {
          "aa_ref": "C",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.287G>A",
          "hgvs_p": "p.Cys96Tyr",
          "transcript": "NM_178850.3",
          "protein_id": "NP_849181.1",
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          "cdna_start": 421,
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          "mane_select": null,
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        },
        {
          "aa_ref": "C",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr",
          "transcript": "NM_001030004.3",
          "protein_id": "NP_001025175.1",
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        },
        {
          "aa_ref": "C",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.212G>A",
          "hgvs_p": "p.Cys71Tyr",
          "transcript": "NM_001287184.2",
          "protein_id": "NP_001274113.1",
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          "cdna_start": 549,
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          "cdna_length": 1780,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
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          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
          "hgvs_c": "c.260G>A",
          "hgvs_p": "p.Cys87Tyr",
          "transcript": "ENST00000619550.5",
          "protein_id": "ENSP00000481331.2",
          "transcript_support_level": 5,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 260,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": 261,
          "cdna_end": null,
          "cdna_length": 1361,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "C",
          "aa_alt": "Y",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "HNF4A",
          "gene_hgnc_id": 5024,
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      ],
      "gene_symbol": "HNF4A",
      "gene_hgnc_id": 5024,
      "dbsnp": "rs1555813342",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
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      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.9929113388061523,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9998,
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      "bayesdelnoaf_score": 0.57,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 9.917,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
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      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PP3,PM2_Supporting,PM1,PP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PP3",
            "PM2_Supporting",
            "PM1",
            "PP4_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000316673.9",
          "gene_symbol": "HNF4A",
          "hgnc_id": 5024,
          "effects": [
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          "inheritance_mode": "AD",
          "hgvs_c": "c.221G>A",
          "hgvs_p": "p.Cys74Tyr"
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      ],
      "clinvar_disease": "Maturity onset diabetes mellitus in young,Monogenic diabetes",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "reviewed by expert panel",
      "clinvar_submissions_summary": "LP:1 US:2",
      "phenotype_combined": "Maturity onset diabetes mellitus in young|Monogenic diabetes",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}