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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-45420577-A-AC (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=45420577&ref=A&alt=AC&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 45420577,
      "ref": "A",
      "alt": "AC",
      "effect": "frameshift_variant",
      "transcript": "ENST00000279036.12",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "NM_015937.6",
          "protein_id": "NP_057021.2",
          "transcript_support_level": null,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 919,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": 935,
          "cdna_end": null,
          "cdna_length": 2168,
          "mane_select": "ENST00000279036.12",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "ENST00000279036.12",
          "protein_id": "ENSP00000279036.6",
          "transcript_support_level": 1,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 919,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": 935,
          "cdna_end": null,
          "cdna_length": 2168,
          "mane_select": "NM_015937.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "ENST00000372689.9",
          "protein_id": "ENSP00000361774.4",
          "transcript_support_level": 1,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 511,
          "cds_start": 919,
          "cds_end": null,
          "cds_length": 1536,
          "cdna_start": 939,
          "cdna_end": null,
          "cdna_length": 1971,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.777dupC",
          "hgvs_p": "p.Val260fs",
          "transcript": "ENST00000639382.1",
          "protein_id": "ENSP00000491534.1",
          "transcript_support_level": 1,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 778,
          "cds_end": null,
          "cds_length": 1180,
          "cdna_start": 791,
          "cdna_end": null,
          "cdna_length": 1193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.639dupC",
          "hgvs_p": "p.Val214fs",
          "transcript": "ENST00000638353.1",
          "protein_id": "ENSP00000491538.1",
          "transcript_support_level": 1,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 317,
          "cds_start": 640,
          "cds_end": null,
          "cds_length": 955,
          "cdna_start": 655,
          "cdna_end": null,
          "cdna_length": 970,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.807dupC",
          "hgvs_p": "p.Val270fs",
          "transcript": "ENST00000639235.1",
          "protein_id": "ENSP00000492498.1",
          "transcript_support_level": 1,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 315,
          "cds_start": 808,
          "cds_end": null,
          "cds_length": 949,
          "cdna_start": 820,
          "cdna_end": null,
          "cdna_length": 961,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.888dupC",
          "hgvs_p": "p.Val297fs",
          "transcript": "ENST00000638489.1",
          "protein_id": "ENSP00000491566.1",
          "transcript_support_level": 1,
          "aa_start": 297,
          "aa_end": null,
          "aa_length": 313,
          "cds_start": 889,
          "cds_end": null,
          "cds_length": 942,
          "cdna_start": 904,
          "cdna_end": null,
          "cdna_length": 957,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "ENST00000639292.2",
          "protein_id": "ENSP00000491678.2",
          "transcript_support_level": 5,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 605,
          "cds_start": 919,
          "cds_end": null,
          "cds_length": 1818,
          "cdna_start": 919,
          "cdna_end": null,
          "cdna_length": 2159,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.924dupC",
          "hgvs_p": "p.Val309fs",
          "transcript": "ENST00000640324.1",
          "protein_id": "ENSP00000491074.1",
          "transcript_support_level": 5,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 580,
          "cds_start": 925,
          "cds_end": null,
          "cds_length": 1743,
          "cdna_start": 925,
          "cdna_end": null,
          "cdna_length": 2098,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "ENST00000638691.2",
          "protein_id": "ENSP00000492094.2",
          "transcript_support_level": 3,
          "aa_start": 307,
          "aa_end": null,
          "aa_length": 550,
          "cds_start": 919,
          "cds_end": null,
          "cds_length": 1653,
          "cdna_start": 935,
          "cdna_end": null,
          "cdna_length": 2082,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.750dupC",
          "hgvs_p": "p.Val251fs",
          "transcript": "NM_001184728.3",
          "protein_id": "NP_001171657.1",
          "transcript_support_level": null,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 751,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": 767,
          "cdna_end": null,
          "cdna_length": 2000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.750dupC",
          "hgvs_p": "p.Val251fs",
          "transcript": "ENST00000543458.7",
          "protein_id": "ENSP00000441577.1",
          "transcript_support_level": 2,
          "aa_start": 251,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 751,
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          "cdna_start": 801,
          "cdna_end": null,
          "cdna_length": 2037,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "NM_001184729.3",
          "protein_id": "NP_001171658.1",
          "transcript_support_level": null,
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          "cds_start": 919,
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          "cdna_start": 935,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
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          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
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          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
          "transcript": "ENST00000638594.1",
          "protein_id": "ENSP00000491697.1",
          "transcript_support_level": 5,
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          "aa_length": 505,
          "cds_start": 919,
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          "cdna_start": 935,
          "cdna_end": null,
          "cdna_length": 1835,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 8,
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          "intron_rank": null,
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          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs",
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          "protein_id": "ENSP00000491170.1",
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          "cdna_start": 935,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.612dupC",
          "hgvs_p": "p.Val205fs",
          "transcript": "NM_001184730.3",
          "protein_id": "NP_001171659.1",
          "transcript_support_level": null,
          "aa_start": 205,
          "aa_end": null,
          "aa_length": 476,
          "cds_start": 613,
          "cds_end": null,
          "cds_length": 1431,
          "cdna_start": 629,
          "cdna_end": null,
          "cdna_length": 1862,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.612dupC",
          "hgvs_p": "p.Val205fs",
          "transcript": "ENST00000279035.14",
          "protein_id": "ENSP00000279035.8",
          "transcript_support_level": 2,
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          "aa_length": 476,
          "cds_start": 613,
          "cds_end": null,
          "cds_length": 1431,
          "cdna_start": 686,
          "cdna_end": null,
          "cdna_length": 1880,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
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          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 5,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
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          "hgvs_p": "p.Val170fs",
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          "cdna_start": 521,
          "cdna_end": null,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.336dupC",
          "hgvs_p": "p.Val113fs",
          "transcript": "ENST00000545755.3",
          "protein_id": "ENSP00000443963.3",
          "transcript_support_level": 5,
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          "aa_length": 384,
          "cds_start": 337,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": 340,
          "cdna_end": null,
          "cdna_length": 1158,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.813dupC",
          "hgvs_p": "p.Val272fs",
          "transcript": "ENST00000638478.1",
          "protein_id": "ENSP00000491233.1",
          "transcript_support_level": 3,
          "aa_start": 272,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 814,
          "cds_end": null,
          "cds_length": 1064,
          "cdna_start": 826,
          "cdna_end": null,
          "cdna_length": 1076,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "R?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PIGT",
          "gene_hgnc_id": 14938,
          "hgvs_c": "c.612dupC",
          "hgvs_p": "p.Val205fs",
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        {
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          "gene_symbol": "PIGT",
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          "hgvs_c": "c.-26_-25insC",
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          "transcript": "ENST00000639239.1",
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        },
        {
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          "gene_symbol": "PIGT",
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      ],
      "gene_symbol": "PIGT",
      "gene_hgnc_id": 14938,
      "dbsnp": "rs751861982",
      "frequency_reference_population": 0.000069408125,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 112,
      "gnomad_exomes_af": 0.0000718259,
      "gnomad_genomes_af": 0.0000461206,
      "gnomad_exomes_ac": 105,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": 0.07999999821186066,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": -1.066,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.08,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 18,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PVS1,PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 18,
          "benign_score": 0,
          "pathogenic_score": 18,
          "criteria": [
            "PVS1",
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000279036.12",
          "gene_symbol": "PIGT",
          "hgnc_id": 14938,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.918dupC",
          "hgvs_p": "p.Val307fs"
        }
      ],
      "clinvar_disease": "Inborn genetic diseases,Multiple congenital anomalies-hypotonia-seizures syndrome 3,PIGT-related disorder,not provided",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:3 O:1",
      "phenotype_combined": "Multiple congenital anomalies-hypotonia-seizures syndrome 3|not provided|PIGT-related disorder|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}