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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-45894040-T-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=45894040&ref=T&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 45894040,
      "ref": "T",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000646241.3",
      "consequences": [
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "NM_000308.4",
          "protein_id": "NP_000299.3",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 790,
          "cdna_end": null,
          "cdna_length": 1858,
          "mane_select": "ENST00000646241.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000646241.3",
          "protein_id": "ENSP00000493613.2",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 790,
          "cdna_end": null,
          "cdna_length": 1858,
          "mane_select": "NM_000308.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.799T>A",
          "hgvs_p": "p.Tyr267Asn",
          "transcript": "ENST00000372484.8",
          "protein_id": "ENSP00000361562.3",
          "transcript_support_level": 1,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 799,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": 1871,
          "cdna_end": null,
          "cdna_length": 2939,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000191018.9",
          "protein_id": "ENSP00000191018.5",
          "transcript_support_level": 1,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 830,
          "cdna_end": null,
          "cdna_length": 1898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000372459.7",
          "protein_id": "ENSP00000361537.2",
          "transcript_support_level": 1,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 928,
          "cdna_end": null,
          "cdna_length": 1996,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.795T>A",
          "hgvs_p": null,
          "transcript": "ENST00000484855.4",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4697,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.799T>A",
          "hgvs_p": "p.Tyr267Asn",
          "transcript": "ENST00000677394.1",
          "protein_id": "ENSP00000504790.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 799,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": 1725,
          "cdna_end": null,
          "cdna_length": 2774,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "NM_001127695.3",
          "protein_id": "NP_001121167.1",
          "transcript_support_level": null,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 836,
          "cdna_end": null,
          "cdna_length": 1904,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000607482.6",
          "protein_id": "ENSP00000475524.2",
          "transcript_support_level": 3,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 819,
          "cdna_end": null,
          "cdna_length": 1887,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.694T>A",
          "hgvs_p": "p.Tyr232Asn",
          "transcript": "NM_001167594.3",
          "protein_id": "NP_001161066.2",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 694,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 739,
          "cdna_end": null,
          "cdna_length": 1807,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.694T>A",
          "hgvs_p": "p.Tyr232Asn",
          "transcript": "ENST00000354880.9",
          "protein_id": "ENSP00000346952.5",
          "transcript_support_level": 2,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 694,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 752,
          "cdna_end": null,
          "cdna_length": 1820,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000678443.1",
          "protein_id": "ENSP00000504006.1",
          "transcript_support_level": null,
          "aa_start": 249,
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          "cds_start": 745,
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          "cdna_start": 745,
          "cdna_end": null,
          "cdna_length": 1697,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "N",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn",
          "transcript": "ENST00000419493.3",
          "protein_id": "ENSP00000408533.3",
          "transcript_support_level": 2,
          "aa_start": 249,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 745,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 771,
          "cdna_end": null,
          "cdna_length": 1778,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.2177T>A",
          "hgvs_p": null,
          "transcript": "ENST00000480961.3",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6079,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.406T>A",
          "hgvs_p": null,
          "transcript": "ENST00000485627.1",
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          "mane_select": null,
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          "biotype": null,
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1112T>A",
          "hgvs_p": null,
          "transcript": "ENST00000493522.8",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2309,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1236T>A",
          "hgvs_p": null,
          "transcript": "ENST00000606066.3",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 2433,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.*387T>A",
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          "transcript": "ENST00000606394.6",
          "protein_id": "ENSP00000475827.1",
          "transcript_support_level": 4,
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          "mane_select": null,
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          "feature": null
        },
        {
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.866T>A",
          "hgvs_p": null,
          "transcript": "ENST00000606782.3",
          "protein_id": null,
          "transcript_support_level": 3,
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          "cdna_length": 2063,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.*110T>A",
          "hgvs_p": null,
          "transcript": "ENST00000606788.5",
          "protein_id": "ENSP00000476235.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3056,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1162T>A",
          "hgvs_p": null,
          "transcript": "ENST00000607187.3",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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      ],
      "gene_symbol": "CTSA",
      "gene_hgnc_id": 9251,
      "dbsnp": "rs137854544",
      "frequency_reference_population": 0.000045876222,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 74,
      "gnomad_exomes_af": 0.0000444957,
      "gnomad_genomes_af": 0.000059125,
      "gnomad_exomes_ac": 65,
      "gnomad_genomes_ac": 9,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6819995641708374,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07000000029802322,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.458,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.2597,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.14,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.744,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.07,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 10,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM2,PP5_Very_Strong",
      "acmg_by_gene": [
        {
          "score": 10,
          "benign_score": 0,
          "pathogenic_score": 10,
          "criteria": [
            "PM2",
            "PP5_Very_Strong"
          ],
          "verdict": "Pathogenic",
          "transcript": "ENST00000646241.3",
          "gene_symbol": "CTSA",
          "hgnc_id": 9251,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.745T>A",
          "hgvs_p": "p.Tyr249Asn"
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      ],
      "clinvar_disease": " late infantile,Combined deficiency of sialidase AND beta galactosidase,Galactosialidosis",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "P:4",
      "phenotype_combined": "Combined deficiency of sialidase AND beta galactosidase|Galactosialidosis, late infantile",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}