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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-45895054-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=45895054&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 45895054,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000646241.3",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "NM_000308.4",
          "protein_id": "NP_000299.3",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1054,
          "cdna_end": null,
          "cdna_length": 1858,
          "mane_select": "ENST00000646241.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "ENST00000646241.3",
          "protein_id": "ENSP00000493613.2",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1054,
          "cdna_end": null,
          "cdna_length": 1858,
          "mane_select": "NM_000308.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1063G>C",
          "hgvs_p": "p.Ala355Pro",
          "transcript": "ENST00000372484.8",
          "protein_id": "ENSP00000361562.3",
          "transcript_support_level": 1,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1063,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": 2135,
          "cdna_end": null,
          "cdna_length": 2939,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "ENST00000191018.9",
          "protein_id": "ENSP00000191018.5",
          "transcript_support_level": 1,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1094,
          "cdna_end": null,
          "cdna_length": 1898,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "ENST00000372459.7",
          "protein_id": "ENSP00000361537.2",
          "transcript_support_level": 1,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1192,
          "cdna_end": null,
          "cdna_length": 1996,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1059G>C",
          "hgvs_p": null,
          "transcript": "ENST00000484855.4",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4697,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1063G>C",
          "hgvs_p": "p.Ala355Pro",
          "transcript": "ENST00000677394.1",
          "protein_id": "ENSP00000504790.1",
          "transcript_support_level": null,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1063,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": 1989,
          "cdna_end": null,
          "cdna_length": 2774,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "NM_001127695.3",
          "protein_id": "NP_001121167.1",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1100,
          "cdna_end": null,
          "cdna_length": 1904,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "ENST00000607482.6",
          "protein_id": "ENSP00000475524.2",
          "transcript_support_level": 3,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 480,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1443,
          "cdna_start": 1083,
          "cdna_end": null,
          "cdna_length": 1887,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.958G>C",
          "hgvs_p": "p.Ala320Pro",
          "transcript": "NM_001167594.3",
          "protein_id": "NP_001161066.2",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 1003,
          "cdna_end": null,
          "cdna_length": 1807,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.958G>C",
          "hgvs_p": "p.Ala320Pro",
          "transcript": "ENST00000354880.9",
          "protein_id": "ENSP00000346952.5",
          "transcript_support_level": 2,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 1016,
          "cdna_end": null,
          "cdna_length": 1820,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro",
          "transcript": "ENST00000678443.1",
          "protein_id": "ENSP00000504006.1",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 450,
          "cds_start": 1009,
          "cds_end": null,
          "cds_length": 1353,
          "cdna_start": 1009,
          "cdna_end": null,
          "cdna_length": 1697,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "c.999G>C",
          "hgvs_p": "p.Leu333Leu",
          "transcript": "ENST00000419493.3",
          "protein_id": "ENSP00000408533.3",
          "transcript_support_level": 2,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 999,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1025,
          "cdna_end": null,
          "cdna_length": 1778,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.2441G>C",
          "hgvs_p": null,
          "transcript": "ENST00000480961.3",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6079,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1376G>C",
          "hgvs_p": null,
          "transcript": "ENST00000493522.8",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2309,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1500G>C",
          "hgvs_p": null,
          "transcript": "ENST00000606066.3",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2433,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.*651G>C",
          "hgvs_p": null,
          "transcript": "ENST00000606394.6",
          "protein_id": "ENSP00000475827.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 992,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1130G>C",
          "hgvs_p": null,
          "transcript": "ENST00000606782.3",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2063,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.*374G>C",
          "hgvs_p": null,
          "transcript": "ENST00000606788.5",
          "protein_id": "ENSP00000476235.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3056,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1426G>C",
          "hgvs_p": null,
          "transcript": "ENST00000607187.3",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4866,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CTSA",
          "gene_hgnc_id": 9251,
          "hgvs_c": "n.1220G>C",
          "hgvs_p": null,
          "transcript": "ENST00000607212.3",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
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      ],
      "gene_symbol": "CTSA",
      "gene_hgnc_id": 9251,
      "dbsnp": "rs374059459",
      "frequency_reference_population": 0.00003283405,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 53,
      "gnomad_exomes_af": 0.0000266779,
      "gnomad_genomes_af": 0.0000919262,
      "gnomad_exomes_ac": 39,
      "gnomad_genomes_ac": 14,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0193842351436615,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.348,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1839,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.27,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.89,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -9,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BS1",
      "acmg_by_gene": [
        {
          "score": -9,
          "benign_score": 9,
          "pathogenic_score": 0,
          "criteria": [
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            "BS1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000646241.3",
          "gene_symbol": "CTSA",
          "hgnc_id": 9251,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1009G>C",
          "hgvs_p": "p.Ala337Pro"
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      ],
      "clinvar_disease": "Combined deficiency of sialidase AND beta galactosidase,Inborn genetic diseases",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:1",
      "phenotype_combined": "Combined deficiency of sialidase AND beta galactosidase|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}