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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 20-63413558-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=20&pos=63413558&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "20",
      "pos": 63413558,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000359125.7",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Lys552Arg",
          "transcript": "NM_172107.4",
          "protein_id": "NP_742105.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 872,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2619,
          "cdna_start": 1847,
          "cdna_end": null,
          "cdna_length": 9247,
          "mane_select": "ENST00000359125.7",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1655A>G",
          "hgvs_p": "p.Lys552Arg",
          "transcript": "ENST00000359125.7",
          "protein_id": "ENSP00000352035.2",
          "transcript_support_level": 1,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 872,
          "cds_start": 1655,
          "cds_end": null,
          "cds_length": 2619,
          "cdna_start": 1847,
          "cdna_end": null,
          "cdna_length": 9247,
          "mane_select": "NM_172107.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1601A>G",
          "hgvs_p": "p.Lys534Arg",
          "transcript": "ENST00000626839.2",
          "protein_id": "ENSP00000486706.1",
          "transcript_support_level": 1,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 854,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 2565,
          "cdna_start": 1815,
          "cdna_end": null,
          "cdna_length": 9213,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1562A>G",
          "hgvs_p": "p.Lys521Arg",
          "transcript": "ENST00000344462.8",
          "protein_id": "ENSP00000339611.4",
          "transcript_support_level": 1,
          "aa_start": 521,
          "aa_end": null,
          "aa_length": 841,
          "cds_start": 1562,
          "cds_end": null,
          "cds_length": 2526,
          "cdna_start": 1608,
          "cdna_end": null,
          "cdna_length": 2750,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1601A>G",
          "hgvs_p": "p.Lys534Arg",
          "transcript": "NM_001382235.1",
          "protein_id": "NP_001369164.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 890,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 2673,
          "cdna_start": 1793,
          "cdna_end": null,
          "cdna_length": 9301,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1601A>G",
          "hgvs_p": "p.Lys534Arg",
          "transcript": "ENST00000706989.1",
          "protein_id": "ENSP00000516702.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 890,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 2673,
          "cdna_start": 1793,
          "cdna_end": null,
          "cdna_length": 9301,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1601A>G",
          "hgvs_p": "p.Lys534Arg",
          "transcript": "NM_172106.3",
          "protein_id": "NP_742104.1",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 854,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 2565,
          "cdna_start": 1793,
          "cdna_end": null,
          "cdna_length": 9193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1598A>G",
          "hgvs_p": "p.Lys533Arg",
          "transcript": "NM_001439003.1",
          "protein_id": "NP_001425932.1",
          "transcript_support_level": null,
          "aa_start": 533,
          "aa_end": null,
          "aa_length": 853,
          "cds_start": 1598,
          "cds_end": null,
          "cds_length": 2562,
          "cdna_start": 1790,
          "cdna_end": null,
          "cdna_length": 9190,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1571A>G",
          "hgvs_p": "p.Lys524Arg",
          "transcript": "NM_004518.6",
          "protein_id": "NP_004509.2",
          "transcript_support_level": null,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 844,
          "cds_start": 1571,
          "cds_end": null,
          "cds_length": 2535,
          "cdna_start": 1763,
          "cdna_end": null,
          "cdna_length": 9163,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1571A>G",
          "hgvs_p": "p.Lys524Arg",
          "transcript": "ENST00000360480.7",
          "protein_id": "ENSP00000353668.3",
          "transcript_support_level": 5,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 844,
          "cds_start": 1571,
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          "cds_length": 2535,
          "cdna_start": 1613,
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          "cdna_length": 2775,
          "mane_select": null,
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        },
        {
          "aa_ref": "K",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1568A>G",
          "hgvs_p": "p.Lys523Arg",
          "transcript": "NM_001439004.1",
          "protein_id": "NP_001425933.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
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          "cds_start": 1568,
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          "cdna_start": 1760,
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          "mane_select": null,
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        {
          "aa_ref": "K",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1568A>G",
          "hgvs_p": "p.Lys523Arg",
          "transcript": "ENST00000627221.3",
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        {
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          ],
          "exon_rank": 14,
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          "gene_symbol": "KCNQ2",
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          "hgvs_c": "c.1562A>G",
          "hgvs_p": "p.Lys521Arg",
          "transcript": "NM_172108.5",
          "protein_id": "NP_742106.1",
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        {
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          "intron_rank": null,
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          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1535A>G",
          "hgvs_p": "p.Lys512Arg",
          "transcript": "ENST00000713605.1",
          "protein_id": "ENSP00000518901.1",
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        {
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          "gene_symbol": "KCNQ2",
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        {
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          "intron_rank": null,
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          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
          "hgvs_c": "c.1535A>G",
          "hgvs_p": "p.Lys512Arg",
          "transcript": "ENST00000625514.2",
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        {
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          "gene_symbol": "KCNQ2",
          "gene_hgnc_id": 6296,
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          "transcript": "ENST00000629241.2",
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        {
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        {
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          "gene_symbol": "KCNQ2",
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        {
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          "gene_symbol": "KCNQ2",
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          "hgvs_c": "c.1571A>G",
          "hgvs_p": "p.Lys524Arg",
          "transcript": "ENST00000629676.2",
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          "mane_select": null,
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        {
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      ],
      "gene_symbol": "KCNQ2",
      "gene_hgnc_id": 6296,
      "dbsnp": "rs1555853613",
      "frequency_reference_population": 6.845199e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.8452e-7,
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      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.8870341181755066,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.14000000059604645,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4889,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.21,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.001,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.14,
      "spliceai_max_prediction": "Benign",
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      "mitotip_score": null,
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      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP2,PP3_Moderate",
      "acmg_by_gene": [
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          "criteria": [
            "PM1",
            "PM2",
            "PP2",
            "PP3_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000359125.7",
          "gene_symbol": "KCNQ2",
          "hgnc_id": 6296,
          "effects": [
            "missense_variant"
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          "inheritance_mode": "AD",
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        {
          "score": 4,
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            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_937002.3",
          "gene_symbol": "LOC105372724",
          "hgnc_id": null,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*208T>C",
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      ],
      "clinvar_disease": "Developmental and epileptic encephalopathy",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Developmental and epileptic encephalopathy",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
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  ],
  "message": null
}