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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-26541443-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=26541443&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 26541443,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001362923.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "NM_003595.5",
          "protein_id": "NP_003586.3",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000338754.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003595.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000338754.9",
          "protein_id": "ENSP00000339813.4",
          "transcript_support_level": 1,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003595.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338754.9"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.350G>A",
          "hgvs_p": "p.Arg117His",
          "transcript": "NM_001362923.2",
          "protein_id": "NP_001349852.1",
          "transcript_support_level": null,
          "aa_start": 117,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 350,
          "cds_end": null,
          "cds_length": 1296,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001362923.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910417.1",
          "protein_id": "ENSP00000580476.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 383,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1152,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910417.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "NM_001008566.3",
          "protein_id": "NP_001008566.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001008566.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "NM_001362922.2",
          "protein_id": "NP_001349851.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001362922.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000398110.6",
          "protein_id": "ENSP00000381180.2",
          "transcript_support_level": 2,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000398110.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000403880.5",
          "protein_id": "ENSP00000385192.1",
          "transcript_support_level": 5,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000403880.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000454778.6",
          "protein_id": "ENSP00000400357.2",
          "transcript_support_level": 4,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000454778.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910410.1",
          "protein_id": "ENSP00000580469.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910410.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910411.1",
          "protein_id": "ENSP00000580470.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910411.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910412.1",
          "protein_id": "ENSP00000580471.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910412.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910413.1",
          "protein_id": "ENSP00000580472.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910413.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910414.1",
          "protein_id": "ENSP00000580473.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910414.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910415.1",
          "protein_id": "ENSP00000580474.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910415.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910416.1",
          "protein_id": "ENSP00000580475.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000910416.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910418.1",
          "protein_id": "ENSP00000580477.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000910418.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910419.1",
          "protein_id": "ENSP00000580478.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910419.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
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          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910421.1",
          "protein_id": "ENSP00000580480.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910421.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.188G>A",
          "hgvs_p": "p.Arg63His",
          "transcript": "ENST00000910422.1",
          "protein_id": "ENSP00000580481.1",
          "transcript_support_level": null,
          "aa_start": 63,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": 188,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910422.1"
        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TPST2",
          "gene_hgnc_id": 12021,
          "hgvs_c": "c.*47G>A",
          "hgvs_p": null,
          "transcript": "ENST00000450022.1",
          "protein_id": "ENSP00000414809.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 46,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 141,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000450022.1"
        }
      ],
      "gene_symbol": "TPST2",
      "gene_hgnc_id": 12021,
      "dbsnp": "rs756408961",
      "frequency_reference_population": 0.0000074945383,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 12,
      "gnomad_exomes_af": 0.00000690173,
      "gnomad_genomes_af": 0.0000131359,
      "gnomad_exomes_ac": 10,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.1481133997440338,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.158,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0799,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.39,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.935,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001362923.2",
          "gene_symbol": "TPST2",
          "hgnc_id": 12021,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.350G>A",
          "hgvs_p": "p.Arg117His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}