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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-30615293-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=30615293&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 30615293,
      "ref": "A",
      "alt": "G",
      "effect": "splice_region_variant,intron_variant",
      "transcript": "NM_000355.4",
      "consequences": [
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "NM_000355.4",
          "protein_id": "NP_000346.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000215838.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000355.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000215838.8",
          "protein_id": "ENSP00000215838.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000355.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000215838.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.500-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000407817.3",
          "protein_id": "ENSP00000384914.3",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000407817.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.603A>G",
          "hgvs_p": "p.Pro201Pro",
          "transcript": "ENST00000698271.1",
          "protein_id": "ENSP00000513642.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 437,
          "cds_start": 603,
          "cds_end": null,
          "cds_length": 1314,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698271.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000947107.1",
          "protein_id": "ENSP00000617166.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 468,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1407,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947107.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000883718.1",
          "protein_id": "ENSP00000553777.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883718.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698268.1",
          "protein_id": "ENSP00000513639.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 436,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1311,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698268.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698266.1",
          "protein_id": "ENSP00000513637.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1296,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698266.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-14A>G",
          "hgvs_p": null,
          "transcript": "ENST00000883717.1",
          "protein_id": "ENSP00000553776.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 425,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1278,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000883717.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-17A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698272.1",
          "protein_id": "ENSP00000513643.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 424,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1275,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698272.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.572-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698273.1",
          "protein_id": "ENSP00000513644.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 424,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1275,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698273.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.569-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000405742.7",
          "protein_id": "ENSP00000385914.3",
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": 423,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1272,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698265.1",
          "protein_id": "ENSP00000513636.1",
          "transcript_support_level": null,
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          "aa_length": 422,
          "cds_start": null,
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          "cds_length": 1269,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698265.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.560-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000947108.1",
          "protein_id": "ENSP00000617167.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": null,
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        },
        {
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          "exon_count": 10,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.506-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000450638.5",
          "protein_id": "ENSP00000394184.2",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": null,
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          "cds_length": 1209,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000450638.5"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.500-8A>G",
          "hgvs_p": null,
          "transcript": "NM_001184726.2",
          "protein_id": "NP_001171655.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "NM_001184726.2"
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.500-17A>G",
          "hgvs_p": null,
          "transcript": "ENST00000883716.1",
          "protein_id": "ENSP00000553775.1",
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          "cds_start": null,
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        },
        {
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          "consequences": [
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            "intron_variant"
          ],
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          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.500-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000947106.1",
          "protein_id": "ENSP00000617165.1",
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          "cds_start": null,
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          "biotype": "protein_coding",
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        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.428-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698270.1",
          "protein_id": "ENSP00000513641.1",
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          "aa_length": 376,
          "cds_start": null,
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          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000698270.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698263.1",
          "protein_id": "ENSP00000513635.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 371,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1116,
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          "protein_id": "ENSP00000597658.1",
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          "protein_coding": false,
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          "cdna_start": null,
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          "biotype": "retained_intron",
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        {
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          "protein_coding": false,
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          "gene_symbol": "TCN2",
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          "hgvs_c": "n.2058-8A>G",
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        {
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            "intron_variant"
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          "exon_rank": null,
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          "exon_count": 8,
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          "gene_symbol": "TCN2",
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          "hgvs_c": "n.581-8A>G",
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          "transcript": "ENST00000698267.1",
          "protein_id": "ENSP00000513638.1",
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          "cdna_start": null,
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          "biotype": "nonsense_mediated_decay",
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        {
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          "protein_coding": false,
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            "splice_region_variant",
            "intron_variant"
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          "exon_count": 8,
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          "gene_symbol": "TCN2",
          "gene_hgnc_id": 11653,
          "hgvs_c": "n.*147-8A>G",
          "hgvs_p": null,
          "transcript": "ENST00000698269.1",
          "protein_id": "ENSP00000513640.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000698269.1"
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      ],
      "gene_symbol": "TCN2",
      "gene_hgnc_id": 11653,
      "dbsnp": "rs7290898",
      "frequency_reference_population": 0.00024848615,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 401,
      "gnomad_exomes_af": 0.000142286,
      "gnomad_genomes_af": 0.00127034,
      "gnomad_exomes_ac": 208,
      "gnomad_genomes_ac": 193,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.9599999785423279,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.0020000000949949026,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.96,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.705,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.09,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.0000136666152544953,
      "dbscsnv_ada_prediction": "Benign",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -9,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BS1",
      "acmg_by_gene": [
        {
          "score": -9,
          "benign_score": 9,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BS1"
          ],
          "verdict": "Benign",
          "transcript": "NM_000355.4",
          "gene_symbol": "TCN2",
          "hgnc_id": 11653,
          "effects": [
            "splice_region_variant",
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.581-8A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Transcobalamin II deficiency",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:1 B:1",
      "phenotype_combined": "Transcobalamin II deficiency",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}