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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 22-50440025-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=22&pos=50440025&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "22",
      "pos": 50440025,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000612753.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp",
          "transcript": "NM_001242898.2",
          "protein_id": "NP_001229827.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": 2721,
          "cdna_end": null,
          "cdna_length": 4094,
          "mane_select": "ENST00000612753.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp",
          "transcript": "ENST00000612753.5",
          "protein_id": "ENSP00000478417.1",
          "transcript_support_level": 2,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": 2721,
          "cdna_end": null,
          "cdna_length": 4094,
          "mane_select": "NM_001242898.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp",
          "transcript": "ENST00000216061.9",
          "protein_id": "ENSP00000216061.5",
          "transcript_support_level": 1,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": 2720,
          "cdna_end": null,
          "cdna_length": 3415,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2272C>T",
          "hgvs_p": "p.Arg758Trp",
          "transcript": "ENST00000395741.7",
          "protein_id": "ENSP00000379090.3",
          "transcript_support_level": 1,
          "aa_start": 758,
          "aa_end": null,
          "aa_length": 933,
          "cds_start": 2272,
          "cds_end": null,
          "cds_length": 2802,
          "cdna_start": 2647,
          "cdna_end": null,
          "cdna_length": 3304,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2269C>T",
          "hgvs_p": "p.Arg757Trp",
          "transcript": "ENST00000395744.7",
          "protein_id": "ENSP00000379093.3",
          "transcript_support_level": 1,
          "aa_start": 757,
          "aa_end": null,
          "aa_length": 932,
          "cds_start": 2269,
          "cds_end": null,
          "cds_length": 2799,
          "cdna_start": 2636,
          "cdna_end": null,
          "cdna_length": 3293,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp",
          "transcript": "NM_001365836.1",
          "protein_id": "NP_001352765.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 966,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2901,
          "cdna_start": 2996,
          "cdna_end": null,
          "cdna_length": 4390,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2353C>T",
          "hgvs_p": "p.Arg785Trp",
          "transcript": "NM_001351641.2",
          "protein_id": "NP_001338570.1",
          "transcript_support_level": null,
          "aa_start": 785,
          "aa_end": null,
          "aa_length": 960,
          "cds_start": 2353,
          "cds_end": null,
          "cds_length": 2883,
          "cdna_start": 2724,
          "cdna_end": null,
          "cdna_length": 4097,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2353C>T",
          "hgvs_p": "p.Arg785Trp",
          "transcript": "NM_001351642.2",
          "protein_id": "NP_001338571.1",
          "transcript_support_level": null,
          "aa_start": 785,
          "aa_end": null,
          "aa_length": 960,
          "cds_start": 2353,
          "cds_end": null,
          "cds_length": 2883,
          "cdna_start": 2724,
          "cdna_end": null,
          "cdna_length": 4097,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp",
          "transcript": "NM_001351643.2",
          "protein_id": "NP_001338572.1",
          "transcript_support_level": null,
          "aa_start": 784,
          "aa_end": null,
          "aa_length": 959,
          "cds_start": 2350,
          "cds_end": null,
          "cds_length": 2880,
          "cdna_start": 2847,
          "cdna_end": null,
          "cdna_length": 4220,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2272C>T",
          "hgvs_p": "p.Arg758Trp",
          "transcript": "NM_001242899.2",
          "protein_id": "NP_001229828.1",
          "transcript_support_level": null,
          "aa_start": 758,
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          "aa_length": 933,
          "cds_start": 2272,
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          "cds_length": 2802,
          "cdna_start": 2643,
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          "mane_select": null,
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          "feature": null
        },
        {
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          "strand": true,
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          ],
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          "exon_count": 23,
          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2272C>T",
          "hgvs_p": "p.Arg758Trp",
          "transcript": "NM_001351644.2",
          "protein_id": "NP_001338573.1",
          "transcript_support_level": null,
          "aa_start": 758,
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          "aa_length": 933,
          "cds_start": 2272,
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          "cdna_start": 2643,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "R",
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          "consequences": [
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          "exon_rank": 21,
          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
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          "hgvs_c": "c.2269C>T",
          "hgvs_p": "p.Arg757Trp",
          "transcript": "NM_001351645.2",
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          "cds_start": 2269,
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          "mane_select": null,
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        {
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          "protein_coding": true,
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          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2269C>T",
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          "transcript": "NM_014678.5",
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        },
        {
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          ],
          "exon_rank": 20,
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          "exon_count": 23,
          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2266C>T",
          "hgvs_p": "p.Arg756Trp",
          "transcript": "NM_001351646.2",
          "protein_id": "NP_001338575.1",
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        {
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          "transcript": "NM_001242900.2",
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        },
        {
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          ],
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          "intron_rank": null,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.2272C>T",
          "hgvs_p": "p.Arg758Trp",
          "transcript": "ENST00000359139.7",
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          "transcript_support_level": 2,
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        {
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          "exon_rank": 21,
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          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.1864C>T",
          "hgvs_p": "p.Arg622Trp",
          "transcript": "NM_001351647.2",
          "protein_id": "NP_001338576.1",
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        {
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          "hgvs_c": "c.1783C>T",
          "hgvs_p": "p.Arg595Trp",
          "transcript": "NM_001351648.2",
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        },
        {
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          "gene_symbol": "PPP6R2",
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          "hgvs_c": "c.1525C>T",
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          "transcript": "ENST00000401672.7",
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        {
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          ],
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          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PPP6R2",
          "gene_hgnc_id": 19253,
          "hgvs_c": "c.1156C>T",
          "hgvs_p": "p.Arg386Trp",
          "transcript": "ENST00000427222.2",
          "protein_id": "ENSP00000391595.2",
          "transcript_support_level": 5,
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          "aa_length": 561,
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          "cdna_start": 1157,
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        },
        {
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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        {
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          "gene_symbol": "PPP6R2",
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          "hgvs_c": "n.273C>T",
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      ],
      "gene_symbol": "PPP6R2",
      "gene_hgnc_id": 19253,
      "dbsnp": "rs193920858",
      "frequency_reference_population": 0.00015435256,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 249,
      "gnomad_exomes_af": 0.000158801,
      "gnomad_genomes_af": 0.000111661,
      "gnomad_exomes_ac": 232,
      "gnomad_genomes_ac": 17,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.11384421586990356,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.086,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0818,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.59,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.133,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000612753.5",
          "gene_symbol": "PPP6R2",
          "hgnc_id": 19253,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2350C>T",
          "hgvs_p": "p.Arg784Trp"
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      ],
      "clinvar_disease": "Prostate cancer",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Prostate cancer",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}