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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-147390969-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=147390969&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 147390969,
      "ref": "C",
      "alt": "A",
      "effect": "synonymous_variant",
      "transcript": "ENST00000383075.8",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.966G>T",
          "hgvs_p": "p.Ser322Ser",
          "transcript": "NM_032153.6",
          "protein_id": "NP_115529.2",
          "transcript_support_level": null,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 966,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 1162,
          "cdna_end": null,
          "cdna_length": 4014,
          "mane_select": "ENST00000383075.8",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.966G>T",
          "hgvs_p": "p.Ser322Ser",
          "transcript": "ENST00000383075.8",
          "protein_id": "ENSP00000372553.3",
          "transcript_support_level": 1,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 966,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 1162,
          "cdna_end": null,
          "cdna_length": 4014,
          "mane_select": "NM_032153.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.1116G>T",
          "hgvs_p": "p.Ser372Ser",
          "transcript": "NM_001168378.1",
          "protein_id": "NP_001161850.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 1116,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": 1302,
          "cdna_end": null,
          "cdna_length": 4152,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.1116G>T",
          "hgvs_p": "p.Ser372Ser",
          "transcript": "ENST00000525172.6",
          "protein_id": "ENSP00000435509.2",
          "transcript_support_level": 2,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 1116,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": 1302,
          "cdna_end": null,
          "cdna_length": 4152,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.1080G>T",
          "hgvs_p": "p.Ser360Ser",
          "transcript": "NM_001168379.2",
          "protein_id": "NP_001161851.1",
          "transcript_support_level": null,
          "aa_start": 360,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 1080,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": 1129,
          "cdna_end": null,
          "cdna_length": 3981,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.1080G>T",
          "hgvs_p": "p.Ser360Ser",
          "transcript": "ENST00000425731.7",
          "protein_id": "ENSP00000397695.3",
          "transcript_support_level": 2,
          "aa_start": 360,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 1080,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": 1129,
          "cdna_end": null,
          "cdna_length": 3979,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.966G>T",
          "hgvs_p": "p.Ser322Ser",
          "transcript": "ENST00000473123.5",
          "protein_id": "ENSP00000420775.1",
          "transcript_support_level": 2,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 966,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 1129,
          "cdna_end": null,
          "cdna_length": 1481,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.966G>T",
          "hgvs_p": "p.Ser322Ser",
          "transcript": "ENST00000484399.5",
          "protein_id": "ENSP00000417855.1",
          "transcript_support_level": 2,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 966,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 1302,
          "cdna_end": null,
          "cdna_length": 1774,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.348G>T",
          "hgvs_p": "p.Ser116Ser",
          "transcript": "NM_001243256.2",
          "protein_id": "NP_001230185.1",
          "transcript_support_level": null,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 348,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": 544,
          "cdna_end": null,
          "cdna_length": 3396,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "c.348G>T",
          "hgvs_p": "p.Ser116Ser",
          "transcript": "ENST00000491672.5",
          "protein_id": "ENSP00000418277.1",
          "transcript_support_level": 4,
          "aa_start": 116,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 348,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": 791,
          "cdna_end": null,
          "cdna_length": 925,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.586G>T",
          "hgvs_p": null,
          "transcript": "ENST00000463850.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 834,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.454G>T",
          "hgvs_p": null,
          "transcript": "ENST00000464502.5",
          "protein_id": null,
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 610,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.700G>T",
          "hgvs_p": null,
          "transcript": "ENST00000472749.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3550,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.652G>T",
          "hgvs_p": null,
          "transcript": "ENST00000475502.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 708,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.1613G>T",
          "hgvs_p": null,
          "transcript": "ENST00000493664.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.1965G>T",
          "hgvs_p": null,
          "transcript": "ENST00000494569.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3317,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.691G>T",
          "hgvs_p": null,
          "transcript": "NR_033118.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3543,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.637G>T",
          "hgvs_p": null,
          "transcript": "NR_033119.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3489,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ZIC4",
          "gene_hgnc_id": 20393,
          "hgvs_c": "n.538G>T",
          "hgvs_p": null,
          "transcript": "NR_040762.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3390,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ZIC4",
      "gene_hgnc_id": 20393,
      "dbsnp": "rs17509456",
      "frequency_reference_population": 0.00006947951,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 112,
      "gnomad_exomes_af": 0.0000691894,
      "gnomad_genomes_af": 0.0000722619,
      "gnomad_exomes_ac": 101,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.3199999928474426,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.69,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Moderate,BP6_Moderate,BP7",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 5,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate",
            "BP6_Moderate",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000383075.8",
          "gene_symbol": "ZIC4",
          "hgnc_id": 20393,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.966G>T",
          "hgvs_p": "p.Ser322Ser"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}