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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-193643378-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=193643378&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 193643378,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_130837.3",
      "consequences": [
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1311A>G",
          "hgvs_p": "p.Ile437Met",
          "transcript": "NM_130837.3",
          "protein_id": "NP_570850.2",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 1015,
          "cds_start": 1311,
          "cds_end": null,
          "cds_length": 3048,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000361510.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130837.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1311A>G",
          "hgvs_p": "p.Ile437Met",
          "transcript": "ENST00000361510.8",
          "protein_id": "ENSP00000355324.2",
          "transcript_support_level": 5,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 1015,
          "cds_start": 1311,
          "cds_end": null,
          "cds_length": 3048,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_130837.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361510.8"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1257A>G",
          "hgvs_p": "p.Ile419Met",
          "transcript": "ENST00000361908.8",
          "protein_id": "ENSP00000354681.3",
          "transcript_support_level": 1,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 1257,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361908.8"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1326A>G",
          "hgvs_p": "p.Ile442Met",
          "transcript": "ENST00000968586.1",
          "protein_id": "ENSP00000638645.1",
          "transcript_support_level": null,
          "aa_start": 442,
          "aa_end": null,
          "aa_length": 1020,
          "cds_start": 1326,
          "cds_end": null,
          "cds_length": 3063,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968586.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1272A>G",
          "hgvs_p": "p.Ile424Met",
          "transcript": "ENST00000968584.1",
          "protein_id": "ENSP00000638643.1",
          "transcript_support_level": null,
          "aa_start": 424,
          "aa_end": null,
          "aa_length": 1002,
          "cds_start": 1272,
          "cds_end": null,
          "cds_length": 3009,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968584.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1257A>G",
          "hgvs_p": "p.Ile419Met",
          "transcript": "NM_130836.3",
          "protein_id": "NP_570849.2",
          "transcript_support_level": null,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 1257,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130836.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1257A>G",
          "hgvs_p": "p.Ile419Met",
          "transcript": "ENST00000890753.1",
          "protein_id": "ENSP00000560812.1",
          "transcript_support_level": null,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 1257,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890753.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1257A>G",
          "hgvs_p": "p.Ile419Met",
          "transcript": "ENST00000925500.1",
          "protein_id": "ENSP00000595559.1",
          "transcript_support_level": null,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 997,
          "cds_start": 1257,
          "cds_end": null,
          "cds_length": 2994,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000925500.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1203A>G",
          "hgvs_p": "p.Ile401Met",
          "transcript": "NM_130835.3",
          "protein_id": "NP_570848.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130835.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1203A>G",
          "hgvs_p": "p.Ile401Met",
          "transcript": "ENST00000361715.6",
          "protein_id": "ENSP00000355311.2",
          "transcript_support_level": 5,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361715.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1149A>G",
          "hgvs_p": "p.Ile383Met",
          "transcript": "ENST00000890756.1",
          "protein_id": "ENSP00000560815.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 1149,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890756.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1203A>G",
          "hgvs_p": "p.Ile401Met",
          "transcript": "ENST00000968579.1",
          "protein_id": "ENSP00000638638.1",
          "transcript_support_level": null,
          "aa_start": 401,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 1203,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000968579.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1200A>G",
          "hgvs_p": "p.Ile400Met",
          "transcript": "NM_130834.3",
          "protein_id": "NP_570847.2",
          "transcript_support_level": null,
          "aa_start": 400,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 1200,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_130834.3"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1200A>G",
          "hgvs_p": "p.Ile400Met",
          "transcript": "ENST00000392437.6",
          "protein_id": "ENSP00000376232.2",
          "transcript_support_level": 3,
          "aa_start": 400,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 1200,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392437.6"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1146A>G",
          "hgvs_p": "p.Ile382Met",
          "transcript": "ENST00000890755.1",
          "protein_id": "ENSP00000560814.1",
          "transcript_support_level": null,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 978,
          "cds_start": 1146,
          "cds_end": null,
          "cds_length": 2937,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890755.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1191A>G",
          "hgvs_p": "p.Ile397Met",
          "transcript": "ENST00000890757.1",
          "protein_id": "ENSP00000560816.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 975,
          "cds_start": 1191,
          "cds_end": null,
          "cds_length": 2928,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890757.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 28,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1146A>G",
          "hgvs_p": "p.Ile382Met",
          "transcript": "ENST00000392436.7",
          "protein_id": "ENSP00000376231.3",
          "transcript_support_level": 3,
          "aa_start": 382,
          "aa_end": null,
          "aa_length": 972,
          "cds_start": 1146,
          "cds_end": null,
          "cds_length": 2919,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000392436.7"
        },
        {
          "aa_ref": "I",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
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          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1311A>G",
          "hgvs_p": "p.Ile437Met",
          "transcript": "ENST00000968581.1",
          "protein_id": "ENSP00000638640.1",
          "transcript_support_level": null,
          "aa_start": 437,
          "aa_end": null,
          "aa_length": 968,
          "cds_start": 1311,
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          "cds_length": 2907,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000968581.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1161A>G",
          "hgvs_p": "p.Ile387Met",
          "transcript": "ENST00000645553.1",
          "protein_id": "ENSP00000494725.1",
          "transcript_support_level": null,
          "aa_start": 387,
          "aa_end": null,
          "aa_length": 965,
          "cds_start": 1161,
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          "cds_length": 2898,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000645553.1"
        },
        {
          "aa_ref": "I",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "OPA1",
          "gene_hgnc_id": 8140,
          "hgvs_c": "c.1149A>G",
          "hgvs_p": "p.Ile383Met",
          "transcript": "NM_130833.3",
          "protein_id": "NP_570846.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 961,
          "cds_start": 1149,
          "cds_end": null,
          "cds_length": 2886,
          "cdna_start": null,
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          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000646699.1"
        }
      ],
      "gene_symbol": "OPA1",
      "gene_hgnc_id": 8140,
      "dbsnp": "rs143319805",
      "frequency_reference_population": 0.00086697255,
      "hom_count_reference_population": 3,
      "allele_count_reference_population": 1394,
      "gnomad_exomes_af": 0.000899289,
      "gnomad_genomes_af": 0.000558109,
      "gnomad_exomes_ac": 1309,
      "gnomad_genomes_ac": 85,
      "gnomad_exomes_homalt": 3,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.37358683347702026,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.943,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.6509,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.53,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 0.888,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP5,BP4,BS1_Supporting,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 6,
          "pathogenic_score": 1,
          "criteria": [
            "PP5",
            "BP4",
            "BS1_Supporting",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_130837.3",
          "gene_symbol": "OPA1",
          "hgnc_id": 8140,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR,SD",
          "hgvs_c": "c.1311A>G",
          "hgvs_p": "p.Ile437Met"
        }
      ],
      "clinvar_disease": " and neuropathy, ataxia, myopathy, ophthalmoplegia,Abortive cerebellar ataxia,Autosomal dominant optic atrophy classic form,Inborn genetic diseases,Mitochondrial DNA depletion syndrome 14 (cardioencephalomyopathic type),OPA1-related disorder,Optic atrophy,Optic atrophy with or without deafness,Optic nerve hypoplasia,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:6 LP:3 US:9",
      "phenotype_combined": "Autosomal dominant optic atrophy classic form|not provided|Abortive cerebellar ataxia|Optic atrophy with or without deafness, ophthalmoplegia, myopathy, ataxia, and neuropathy;Autosomal dominant optic atrophy classic form;Abortive cerebellar ataxia|Optic atrophy|Optic atrophy with or without deafness, ophthalmoplegia, myopathy, ataxia, and neuropathy;Autosomal dominant optic atrophy classic form;Mitochondrial DNA depletion syndrome 14 (cardioencephalomyopathic type);Abortive cerebellar ataxia|not specified|OPA1-related disorder|Optic nerve hypoplasia|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}