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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 3-50269320-G-GC (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=3&pos=50269320&ref=G&alt=GC&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "3",
      "pos": 50269320,
      "ref": "G",
      "alt": "GC",
      "effect": "frameshift_variant",
      "transcript": "NM_001290061.1",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "NM_001290060.2",
          "protein_id": "NP_001276989.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000616701.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001290060.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000616701.5",
          "protein_id": "ENSP00000484146.1",
          "transcript_support_level": 1,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001290060.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000616701.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000611067.4",
          "protein_id": "ENSP00000480680.1",
          "transcript_support_level": 1,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000611067.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000433753.4",
          "protein_id": "ENSP00000485281.1",
          "transcript_support_level": 1,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 748,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2247,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000433753.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942600.1",
          "protein_id": "ENSP00000612659.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 756,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2271,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942600.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942595.1",
          "protein_id": "ENSP00000612654.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 755,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2268,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942595.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "NM_001290061.1",
          "protein_id": "NP_001276990.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001290061.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "NM_001435956.1",
          "protein_id": "NP_001422885.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001435956.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "NM_004636.4",
          "protein_id": "NP_004627.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004636.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000618865.4",
          "protein_id": "ENSP00000481957.1",
          "transcript_support_level": 5,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000618865.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852511.1",
          "protein_id": "ENSP00000522570.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852511.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852512.1",
          "protein_id": "ENSP00000522571.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852512.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852513.1",
          "protein_id": "ENSP00000522572.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852513.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852514.1",
          "protein_id": "ENSP00000522573.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852514.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852515.1",
          "protein_id": "ENSP00000522574.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852515.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000852516.1",
          "protein_id": "ENSP00000522575.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000852516.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942593.1",
          "protein_id": "ENSP00000612652.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942593.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942594.1",
          "protein_id": "ENSP00000612653.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942594.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942596.1",
          "protein_id": "ENSP00000612655.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
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          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942596.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P?",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "frameshift_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEMA3B",
          "gene_hgnc_id": 10724,
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs",
          "transcript": "ENST00000942597.1",
          "protein_id": "ENSP00000612656.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 749,
          "cds_start": 87,
          "cds_end": null,
          "cds_length": 2250,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942597.1"
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        {
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        {
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        },
        {
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          "gene_symbol": "SEMA3B-AS1",
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          "biotype": "pseudogene",
          "feature": "ENST00000736629.1"
        }
      ],
      "gene_symbol": "SEMA3B",
      "gene_hgnc_id": 10724,
      "dbsnp": "rs67324803",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": 0,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 0,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": null,
      "computational_prediction_selected": null,
      "computational_source_selected": null,
      "splice_score_selected": null,
      "splice_prediction_selected": null,
      "splice_source_selected": null,
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": null,
      "bayesdelnoaf_prediction": null,
      "phylop100way_score": -0.055,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": null,
      "spliceai_max_prediction": null,
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP6_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP6_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001290061.1",
          "gene_symbol": "SEMA3B",
          "hgnc_id": 10724,
          "effects": [
            "frameshift_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.86dupC",
          "hgvs_p": "p.Arg30fs"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP6_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000736627.1",
          "gene_symbol": "SEMA3B-AS1",
          "hgnc_id": 49096,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.134-2434dupG",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "SEMA3B-related disorder",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "B:1",
      "phenotype_combined": "SEMA3B-related disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}