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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-1978885-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=1978885&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 1978885,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001440893.1",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "NM_001042424.3",
          "protein_id": "NP_001035889.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000508803.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001042424.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000508803.6",
          "protein_id": "ENSP00000423972.1",
          "transcript_support_level": 1,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001042424.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000508803.6"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000382892.6",
          "protein_id": "ENSP00000372348.2",
          "transcript_support_level": 1,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000382892.6"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000382895.7",
          "protein_id": "ENSP00000372351.3",
          "transcript_support_level": 1,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000382895.7"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.2118G>T",
          "hgvs_p": "p.Trp706Cys",
          "transcript": "ENST00000382888.3",
          "protein_id": "ENSP00000372344.3",
          "transcript_support_level": 1,
          "aa_start": 706,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 2118,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000382888.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "n.*2357G>T",
          "hgvs_p": null,
          "transcript": "ENST00000312087.10",
          "protein_id": "ENSP00000308780.6",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000312087.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "n.*2224G>T",
          "hgvs_p": null,
          "transcript": "ENST00000353275.9",
          "protein_id": "ENSP00000329167.5",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000353275.9"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "n.2691G>T",
          "hgvs_p": null,
          "transcript": "ENST00000482415.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000482415.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "n.*2357G>T",
          "hgvs_p": null,
          "transcript": "ENST00000312087.10",
          "protein_id": "ENSP00000308780.6",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000312087.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "n.*2224G>T",
          "hgvs_p": null,
          "transcript": "ENST00000353275.9",
          "protein_id": "ENSP00000329167.5",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000353275.9"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4173G>T",
          "hgvs_p": "p.Trp1391Cys",
          "transcript": "NM_001440893.1",
          "protein_id": "NP_001427822.1",
          "transcript_support_level": null,
          "aa_start": 1391,
          "aa_end": null,
          "aa_length": 1398,
          "cds_start": 4173,
          "cds_end": null,
          "cds_length": 4197,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440893.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "NM_001440892.1",
          "protein_id": "NP_001427821.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440892.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "NM_133330.3",
          "protein_id": "NP_579877.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_133330.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "NM_133331.3",
          "protein_id": "NP_579878.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_133331.3"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "NM_133335.4",
          "protein_id": "NP_579890.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_133335.4"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000382891.9",
          "protein_id": "ENSP00000372347.5",
          "transcript_support_level": 5,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000382891.9"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000899091.1",
          "protein_id": "ENSP00000569150.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899091.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000899092.1",
          "protein_id": "ENSP00000569151.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000899092.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000922145.1",
          "protein_id": "ENSP00000592204.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
          "cds_length": 4098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922145.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 23,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NSD2",
          "gene_hgnc_id": 12766,
          "hgvs_c": "c.4074G>T",
          "hgvs_p": "p.Trp1358Cys",
          "transcript": "ENST00000922150.1",
          "protein_id": "ENSP00000592209.1",
          "transcript_support_level": null,
          "aa_start": 1358,
          "aa_end": null,
          "aa_length": 1365,
          "cds_start": 4074,
          "cds_end": null,
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      "dbsnp": "rs1553881444",
      "frequency_reference_population": 7.017534e-7,
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      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.01753e-7,
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      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.4942961037158966,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.567,
      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.09,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.127,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001440893.1",
          "gene_symbol": "NSD2",
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          "effects": [
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          "hgvs_p": "p.Trp1391Cys"
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      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}