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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 4-39300409-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=4&pos=39300409&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "4",
      "pos": 39300409,
      "ref": "T",
      "alt": "C",
      "effect": "synonymous_variant",
      "transcript": "NM_001204747.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2541A>G",
          "hgvs_p": "p.Pro847Pro",
          "transcript": "NM_002913.5",
          "protein_id": "NP_002904.3",
          "transcript_support_level": null,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 2541,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": 2662,
          "cdna_end": null,
          "cdna_length": 4870,
          "mane_select": "ENST00000349703.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002913.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2541A>G",
          "hgvs_p": "p.Pro847Pro",
          "transcript": "ENST00000349703.7",
          "protein_id": "ENSP00000261424.4",
          "transcript_support_level": 1,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 1147,
          "cds_start": 2541,
          "cds_end": null,
          "cds_length": 3444,
          "cdna_start": 2662,
          "cdna_end": null,
          "cdna_length": 4870,
          "mane_select": "NM_002913.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000349703.7"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2544A>G",
          "hgvs_p": "p.Pro848Pro",
          "transcript": "ENST00000381897.5",
          "protein_id": "ENSP00000371321.1",
          "transcript_support_level": 1,
          "aa_start": 848,
          "aa_end": null,
          "aa_length": 1148,
          "cds_start": 2544,
          "cds_end": null,
          "cds_length": 3447,
          "cdna_start": 2678,
          "cdna_end": null,
          "cdna_length": 4886,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381897.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2544A>G",
          "hgvs_p": "p.Pro848Pro",
          "transcript": "ENST00000906184.1",
          "protein_id": "ENSP00000576243.1",
          "transcript_support_level": null,
          "aa_start": 848,
          "aa_end": null,
          "aa_length": 1163,
          "cds_start": 2544,
          "cds_end": null,
          "cds_length": 3492,
          "cdna_start": 2668,
          "cdna_end": null,
          "cdna_length": 4921,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906184.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2577A>G",
          "hgvs_p": "p.Pro859Pro",
          "transcript": "ENST00000967837.1",
          "protein_id": "ENSP00000637896.1",
          "transcript_support_level": null,
          "aa_start": 859,
          "aa_end": null,
          "aa_length": 1159,
          "cds_start": 2577,
          "cds_end": null,
          "cds_length": 3480,
          "cdna_start": 2698,
          "cdna_end": null,
          "cdna_length": 4904,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000967837.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2544A>G",
          "hgvs_p": "p.Pro848Pro",
          "transcript": "NM_001204747.2",
          "protein_id": "NP_001191676.1",
          "transcript_support_level": null,
          "aa_start": 848,
          "aa_end": null,
          "aa_length": 1148,
          "cds_start": 2544,
          "cds_end": null,
          "cds_length": 3447,
          "cdna_start": 2665,
          "cdna_end": null,
          "cdna_length": 4873,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001204747.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2541A>G",
          "hgvs_p": "p.Pro847Pro",
          "transcript": "ENST00000923045.1",
          "protein_id": "ENSP00000593103.1",
          "transcript_support_level": null,
          "aa_start": 847,
          "aa_end": null,
          "aa_length": 1130,
          "cds_start": 2541,
          "cds_end": null,
          "cds_length": 3393,
          "cdna_start": 2647,
          "cdna_end": null,
          "cdna_length": 4803,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000923045.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2466A>G",
          "hgvs_p": "p.Pro822Pro",
          "transcript": "NM_001363496.2",
          "protein_id": "NP_001350425.1",
          "transcript_support_level": null,
          "aa_start": 822,
          "aa_end": null,
          "aa_length": 1122,
          "cds_start": 2466,
          "cds_end": null,
          "cds_length": 3369,
          "cdna_start": 2587,
          "cdna_end": null,
          "cdna_length": 4795,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363496.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2466A>G",
          "hgvs_p": "p.Pro822Pro",
          "transcript": "ENST00000906185.1",
          "protein_id": "ENSP00000576244.1",
          "transcript_support_level": null,
          "aa_start": 822,
          "aa_end": null,
          "aa_length": 1122,
          "cds_start": 2466,
          "cds_end": null,
          "cds_length": 3369,
          "cdna_start": 2587,
          "cdna_end": null,
          "cdna_length": 4035,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000906185.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
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          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2466A>G",
          "hgvs_p": "p.Pro822Pro",
          "transcript": "ENST00000923043.1",
          "protein_id": "ENSP00000593102.1",
          "transcript_support_level": null,
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          "cds_start": 2466,
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          "cdna_start": 2587,
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          "mane_select": null,
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        },
        {
          "aa_ref": "P",
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          "canonical": false,
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 19,
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          "intron_rank": null,
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          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "c.2463A>G",
          "hgvs_p": "p.Pro821Pro",
          "transcript": "NM_001363495.2",
          "protein_id": "NP_001350424.1",
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          "cds_start": 2463,
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          "cdna_start": 2584,
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          "biotype": "protein_coding",
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        },
        {
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          "gene_symbol": "RFC1",
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          "hgvs_c": "c.2463A>G",
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          "transcript": "ENST00000906183.1",
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        },
        {
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          "strand": false,
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          ],
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          "gene_symbol": "RFC1",
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          ],
          "exon_rank": 18,
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          "intron_rank": null,
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          "gene_symbol": "RFC1",
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          "hgvs_c": "c.2388A>G",
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          "transcript": "ENST00000923042.1",
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        {
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          "gene_symbol": "RFC1",
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          "transcript": "ENST00000967836.1",
          "protein_id": "ENSP00000637895.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000967836.1"
        },
        {
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          "strand": false,
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          ],
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          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "RFC1",
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          "hgvs_p": "p.Pro583Pro",
          "transcript": "ENST00000923046.1",
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        {
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          "strand": false,
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          ],
          "exon_rank": 18,
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          "exon_count": 23,
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          "gene_symbol": "RFC1",
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          "transcript": "XM_011513731.2",
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        {
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          ],
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          "gene_symbol": "RFC1",
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          "hgvs_c": "c.1956A>G",
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          "protein_id": "XP_047272010.1",
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        },
        {
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          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RFC1",
          "gene_hgnc_id": 9969,
          "hgvs_c": "n.473A>G",
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          "transcript": "ENST00000505077.1",
          "protein_id": null,
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          "cdna_start": null,
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          "cdna_length": 566,
          "mane_select": null,
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          "biotype": "retained_intron",
          "feature": "ENST00000505077.1"
        }
      ],
      "gene_symbol": "RFC1",
      "gene_hgnc_id": 9969,
      "dbsnp": "rs2066786",
      "frequency_reference_population": 0.5514715,
      "hom_count_reference_population": 253413,
      "allele_count_reference_population": 888758,
      "gnomad_exomes_af": 0.561697,
      "gnomad_genomes_af": 0.453286,
      "gnomad_exomes_ac": 819854,
      "gnomad_genomes_ac": 68904,
      "gnomad_exomes_homalt": 234752,
      "gnomad_genomes_homalt": 18661,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.7799999713897705,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.78,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.951,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -21,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BP7,BA1",
      "acmg_by_gene": [
        {
          "score": -21,
          "benign_score": 21,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BP7",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001204747.2",
          "gene_symbol": "RFC1",
          "hgnc_id": 9969,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2544A>G",
          "hgvs_p": "p.Pro848Pro"
        }
      ],
      "clinvar_disease": "Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome,RFC1-related disorder,not provided",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:2",
      "phenotype_combined": "Cerebellar ataxia with neuropathy and bilateral vestibular areflexia syndrome|RFC1-related disorder|not provided",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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