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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-126559262-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=126559262&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 126559262,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001182.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "NM_001182.5",
          "protein_id": "NP_001173.2",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 539,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1620,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000409134.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001182.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000409134.8",
          "protein_id": "ENSP00000387123.3",
          "transcript_support_level": 1,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 539,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1620,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001182.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409134.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.1031G>C",
          "hgvs_p": "p.Arg344Thr",
          "transcript": "ENST00000636879.1",
          "protein_id": "ENSP00000490811.1",
          "transcript_support_level": 5,
          "aa_start": 344,
          "aa_end": null,
          "aa_length": 554,
          "cds_start": 1031,
          "cds_end": null,
          "cds_length": 1665,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636879.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.1028G>C",
          "hgvs_p": "p.Arg343Thr",
          "transcript": "ENST00000939100.1",
          "protein_id": "ENSP00000609159.1",
          "transcript_support_level": null,
          "aa_start": 343,
          "aa_end": null,
          "aa_length": 553,
          "cds_start": 1028,
          "cds_end": null,
          "cds_length": 1662,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939100.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000865294.1",
          "protein_id": "ENSP00000535353.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 549,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1650,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865294.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.1007G>C",
          "hgvs_p": "p.Arg336Thr",
          "transcript": "ENST00000865298.1",
          "protein_id": "ENSP00000535357.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 546,
          "cds_start": 1007,
          "cds_end": null,
          "cds_length": 1641,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865298.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.983G>C",
          "hgvs_p": "p.Arg328Thr",
          "transcript": "ENST00000635851.1",
          "protein_id": "ENSP00000490819.1",
          "transcript_support_level": 5,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1635,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000635851.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000939101.1",
          "protein_id": "ENSP00000609160.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 538,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1617,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000939101.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.977G>C",
          "hgvs_p": "p.Arg326Thr",
          "transcript": "ENST00000637272.1",
          "protein_id": "ENSP00000489686.1",
          "transcript_support_level": 5,
          "aa_start": 326,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 977,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637272.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000865293.1",
          "protein_id": "ENSP00000535352.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865293.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.947G>C",
          "hgvs_p": "p.Arg316Thr",
          "transcript": "ENST00000865287.1",
          "protein_id": "ENSP00000535346.1",
          "transcript_support_level": null,
          "aa_start": 316,
          "aa_end": null,
          "aa_length": 526,
          "cds_start": 947,
          "cds_end": null,
          "cds_length": 1581,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865287.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.944G>C",
          "hgvs_p": "p.Arg315Thr",
          "transcript": "ENST00000865296.1",
          "protein_id": "ENSP00000535355.1",
          "transcript_support_level": null,
          "aa_start": 315,
          "aa_end": null,
          "aa_length": 525,
          "cds_start": 944,
          "cds_end": null,
          "cds_length": 1578,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865296.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000637782.1",
          "protein_id": "ENSP00000490024.1",
          "transcript_support_level": 5,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 521,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1566,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637782.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.908G>C",
          "hgvs_p": "p.Arg303Thr",
          "transcript": "ENST00000865295.1",
          "protein_id": "ENSP00000535354.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 908,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865295.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
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          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.902G>C",
          "hgvs_p": "p.Arg301Thr",
          "transcript": "NM_001201377.2",
          "protein_id": "NP_001188306.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 511,
          "cds_start": 902,
          "cds_end": null,
          "cds_length": 1536,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001201377.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.884G>C",
          "hgvs_p": "p.Arg295Thr",
          "transcript": "ENST00000865291.1",
          "protein_id": "ENSP00000535350.1",
          "transcript_support_level": null,
          "aa_start": 295,
          "aa_end": null,
          "aa_length": 505,
          "cds_start": 884,
          "cds_end": null,
          "cds_length": 1518,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865291.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000865288.1",
          "protein_id": "ENSP00000535347.1",
          "transcript_support_level": null,
          "aa_start": 329,
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          "cds_start": 986,
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          "cds_length": 1503,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.866G>C",
          "hgvs_p": "p.Arg289Thr",
          "transcript": "ENST00000636743.1",
          "protein_id": "ENSP00000489725.1",
          "transcript_support_level": 5,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 499,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1500,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636743.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
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          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.863G>C",
          "hgvs_p": "p.Arg288Thr",
          "transcript": "ENST00000865292.1",
          "protein_id": "ENSP00000535351.1",
          "transcript_support_level": null,
          "aa_start": 288,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 863,
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          "cds_length": 1497,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000865292.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALDH7A1",
          "gene_hgnc_id": 877,
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr",
          "transcript": "ENST00000960036.1",
          "protein_id": "ENSP00000630095.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 489,
          "cds_start": 986,
          "cds_end": null,
          "cds_length": 1470,
          "cdna_start": null,
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      "gene_symbol": "ALDH7A1",
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      "dbsnp": "rs864622558",
      "frequency_reference_population": 6.841649e-7,
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      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84165e-7,
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      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9290840029716492,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9979,
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      "bayesdelnoaf_score": 0.36,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.889,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 11,
      "acmg_classification": "Pathogenic",
      "acmg_criteria": "PM1,PM2,PM5,PP2,PP3_Moderate,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 11,
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          "criteria": [
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            "PM2",
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            "PP2",
            "PP3_Moderate",
            "PP5_Moderate"
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          "verdict": "Pathogenic",
          "transcript": "NM_001182.5",
          "gene_symbol": "ALDH7A1",
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          "effects": [
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          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.986G>C",
          "hgvs_p": "p.Arg329Thr"
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      ],
      "clinvar_disease": "Pyridoxine-dependent epilepsy",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Pyridoxine-dependent epilepsy",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}