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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-134115992-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=134115992&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 134115992,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001346425.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "NM_003202.5",
          "protein_id": "NP_003193.2",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000342854.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_003202.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "ENST00000342854.10",
          "protein_id": "ENSP00000340347.5",
          "transcript_support_level": 1,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_003202.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000342854.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000395023.5",
          "protein_id": "ENSP00000378469.1",
          "transcript_support_level": 1,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395023.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000518915.5",
          "protein_id": "ENSP00000430179.1",
          "transcript_support_level": 1,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000518915.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000378560.8",
          "protein_id": "ENSP00000367822.4",
          "transcript_support_level": 1,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 268,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 807,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000378560.8"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "ENST00000395029.5",
          "protein_id": "ENSP00000378472.1",
          "transcript_support_level": 5,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 481,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1446,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000395029.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "NM_001346425.2",
          "protein_id": "NP_001333354.1",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001346425.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "ENST00000851078.1",
          "protein_id": "ENSP00000521137.1",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851078.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000522375.6",
          "protein_id": "ENSP00000427870.2",
          "transcript_support_level": 4,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 300,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 903,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000522375.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "NM_001346450.2",
          "protein_id": "NP_001333379.1",
          "transcript_support_level": null,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 281,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 846,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001346450.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "NM_001134851.4",
          "protein_id": "NP_001128323.2",
          "transcript_support_level": null,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001134851.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "NM_201632.5",
          "protein_id": "NP_963963.1",
          "transcript_support_level": null,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 810,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_201632.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "NM_213648.5",
          "protein_id": "NP_998813.1",
          "transcript_support_level": null,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_213648.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000520958.5",
          "protein_id": "ENSP00000429547.1",
          "transcript_support_level": 2,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 269,
          "cds_start": 55,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "NM_201634.5",
          "protein_id": "NP_963965.1",
          "transcript_support_level": null,
          "aa_start": 19,
          "aa_end": null,
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          "cds_start": 55,
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          "cds_length": 807,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_201634.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000517851.5",
          "protein_id": "ENSP00000429946.1",
          "transcript_support_level": 4,
          "aa_start": 19,
          "aa_end": null,
          "aa_length": 166,
          "cds_start": 55,
          "cds_end": null,
          "cds_length": 503,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000517851.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000521639.5",
          "protein_id": "ENSP00000427782.1",
          "transcript_support_level": 4,
          "aa_start": 19,
          "aa_end": null,
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          "cds_start": 55,
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          "cds_length": 442,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "P",
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.55C>T",
          "hgvs_p": "p.Pro19Ser",
          "transcript": "ENST00000518887.5",
          "protein_id": "ENSP00000430617.1",
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "protein_coding": true,
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            "missense_variant"
          ],
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          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "XM_006714678.4",
          "protein_id": "XP_006714741.1",
          "transcript_support_level": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_006714678.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser",
          "transcript": "XM_006714679.4",
          "protein_id": "XP_006714742.1",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 400,
          "cds_end": null,
          "cds_length": 1290,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_006714679.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
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          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "n.120C>T",
          "hgvs_p": null,
          "transcript": "ENST00000522653.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000522653.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TCF7",
          "gene_hgnc_id": 11639,
          "hgvs_c": "n.380C>T",
          "hgvs_p": null,
          "transcript": "NR_033449.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_033449.3"
        }
      ],
      "gene_symbol": "TCF7",
      "gene_hgnc_id": 11639,
      "dbsnp": "rs5742913",
      "frequency_reference_population": 0.000011155499,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 18,
      "gnomad_exomes_af": 0.00000958026,
      "gnomad_genomes_af": 0.0000262784,
      "gnomad_exomes_ac": 14,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.06779026985168457,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.374,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0606,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.15,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -1.708,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -8,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BS2",
      "acmg_by_gene": [
        {
          "score": -8,
          "benign_score": 8,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001346425.2",
          "gene_symbol": "TCF7",
          "hgnc_id": 11639,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.400C>T",
          "hgvs_p": "p.Pro134Ser"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}