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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 5-256367-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=5&pos=256367&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "5",
      "pos": 256367,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_004168.4",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1942A>C",
          "hgvs_p": "p.Thr648Pro",
          "transcript": "NM_004168.4",
          "protein_id": "NP_004159.2",
          "transcript_support_level": null,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1942,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000264932.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004168.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1942A>C",
          "hgvs_p": "p.Thr648Pro",
          "transcript": "ENST00000264932.11",
          "protein_id": "ENSP00000264932.6",
          "transcript_support_level": 1,
          "aa_start": 648,
          "aa_end": null,
          "aa_length": 664,
          "cds_start": 1942,
          "cds_end": null,
          "cds_length": 1995,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004168.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264932.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*675A>C",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 24,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000286001",
          "gene_hgnc_id": null,
          "hgvs_c": "n.*675A>C",
          "hgvs_p": null,
          "transcript": "ENST00000651543.1",
          "protein_id": "ENSP00000499215.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000651543.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2128A>C",
          "hgvs_p": "p.Thr710Pro",
          "transcript": "ENST00000874235.1",
          "protein_id": "ENSP00000544294.1",
          "transcript_support_level": null,
          "aa_start": 710,
          "aa_end": null,
          "aa_length": 726,
          "cds_start": 2128,
          "cds_end": null,
          "cds_length": 2181,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874235.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.2095A>C",
          "hgvs_p": "p.Thr699Pro",
          "transcript": "ENST00000952715.1",
          "protein_id": "ENSP00000622774.1",
          "transcript_support_level": null,
          "aa_start": 699,
          "aa_end": null,
          "aa_length": 715,
          "cds_start": 2095,
          "cds_end": null,
          "cds_length": 2148,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952715.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1996A>C",
          "hgvs_p": "p.Thr666Pro",
          "transcript": "ENST00000874242.1",
          "protein_id": "ENSP00000544301.1",
          "transcript_support_level": null,
          "aa_start": 666,
          "aa_end": null,
          "aa_length": 682,
          "cds_start": 1996,
          "cds_end": null,
          "cds_length": 2049,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874242.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1993A>C",
          "hgvs_p": "p.Thr665Pro",
          "transcript": "ENST00000952713.1",
          "protein_id": "ENSP00000622772.1",
          "transcript_support_level": null,
          "aa_start": 665,
          "aa_end": null,
          "aa_length": 681,
          "cds_start": 1993,
          "cds_end": null,
          "cds_length": 2046,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952713.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1969A>C",
          "hgvs_p": "p.Thr657Pro",
          "transcript": "ENST00000952712.1",
          "protein_id": "ENSP00000622771.1",
          "transcript_support_level": null,
          "aa_start": 657,
          "aa_end": null,
          "aa_length": 673,
          "cds_start": 1969,
          "cds_end": null,
          "cds_length": 2022,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952712.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1966A>C",
          "hgvs_p": "p.Thr656Pro",
          "transcript": "ENST00000874248.1",
          "protein_id": "ENSP00000544307.1",
          "transcript_support_level": null,
          "aa_start": 656,
          "aa_end": null,
          "aa_length": 672,
          "cds_start": 1966,
          "cds_end": null,
          "cds_length": 2019,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874248.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1948A>C",
          "hgvs_p": "p.Thr650Pro",
          "transcript": "ENST00000952708.1",
          "protein_id": "ENSP00000622767.1",
          "transcript_support_level": null,
          "aa_start": 650,
          "aa_end": null,
          "aa_length": 666,
          "cds_start": 1948,
          "cds_end": null,
          "cds_length": 2001,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952708.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
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          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1939A>C",
          "hgvs_p": "p.Thr647Pro",
          "transcript": "ENST00000874234.1",
          "protein_id": "ENSP00000544293.1",
          "transcript_support_level": null,
          "aa_start": 647,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 1939,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874234.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1939A>C",
          "hgvs_p": "p.Thr647Pro",
          "transcript": "ENST00000952707.1",
          "protein_id": "ENSP00000622766.1",
          "transcript_support_level": null,
          "aa_start": 647,
          "aa_end": null,
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          "cds_start": 1939,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000952707.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1918A>C",
          "hgvs_p": "p.Thr640Pro",
          "transcript": "ENST00000874246.1",
          "protein_id": "ENSP00000544305.1",
          "transcript_support_level": null,
          "aa_start": 640,
          "aa_end": null,
          "aa_length": 656,
          "cds_start": 1918,
          "cds_end": null,
          "cds_length": 1971,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874246.1"
        },
        {
          "aa_ref": "T",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "SDHA",
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          "hgvs_c": "c.1909A>C",
          "hgvs_p": "p.Thr637Pro",
          "transcript": "ENST00000874244.1",
          "protein_id": "ENSP00000544303.1",
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          "aa_start": 637,
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          "cds_start": 1909,
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          "cds_length": 1962,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874244.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1909A>C",
          "hgvs_p": "p.Thr637Pro",
          "transcript": "ENST00000952709.1",
          "protein_id": "ENSP00000622768.1",
          "transcript_support_level": null,
          "aa_start": 637,
          "aa_end": null,
          "aa_length": 653,
          "cds_start": 1909,
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          "cds_length": 1962,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000952709.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1897A>C",
          "hgvs_p": "p.Thr633Pro",
          "transcript": "ENST00000952716.1",
          "protein_id": "ENSP00000622775.1",
          "transcript_support_level": null,
          "aa_start": 633,
          "aa_end": null,
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          "cds_start": 1897,
          "cds_end": null,
          "cds_length": 1950,
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          "biotype": "protein_coding",
          "feature": "ENST00000952716.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
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          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1891A>C",
          "hgvs_p": "p.Thr631Pro",
          "transcript": "ENST00000952711.1",
          "protein_id": "ENSP00000622770.1",
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          "aa_length": 647,
          "cds_start": 1891,
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          "cds_length": 1944,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000952711.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1828A>C",
          "hgvs_p": "p.Thr610Pro",
          "transcript": "ENST00000874236.1",
          "protein_id": "ENSP00000544295.1",
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          "aa_start": 610,
          "aa_end": null,
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          "cds_start": 1828,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874236.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SDHA",
          "gene_hgnc_id": 10680,
          "hgvs_c": "c.1798A>C",
          "hgvs_p": "p.Thr600Pro",
          "transcript": "NM_001294332.2",
          "protein_id": "NP_001281261.1",
          "transcript_support_level": null,
          "aa_start": 600,
          "aa_end": null,
          "aa_length": 616,
          "cds_start": 1798,
          "cds_end": null,
          "cds_length": 1851,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001294332.2"
        },
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          "gene_symbol": "SDHA",
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          "transcript": "ENST00000514027.5",
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          "biotype": "retained_intron",
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        {
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          "gene_symbol": "SDHA",
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        },
        {
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            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 16,
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          "gene_symbol": "SDHA",
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          "hgvs_c": "n.1944+1861A>C",
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          "transcript": "XR_007058614.1",
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          "biotype": "pseudogene",
          "feature": "XR_007058614.1"
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      ],
      "gene_symbol": "SDHA",
      "gene_hgnc_id": 10680,
      "dbsnp": "rs758054627",
      "frequency_reference_population": 0.00006134483,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 99,
      "gnomad_exomes_af": 0.00006705,
      "gnomad_genomes_af": 0.00000656892,
      "gnomad_exomes_ac": 98,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5689453482627869,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.633,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.247,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.312,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 0,
          "pathogenic_score": 0,
          "criteria": [],
          "verdict": "Uncertain_significance",
          "transcript": "NM_004168.4",
          "gene_symbol": "SDHA",
          "hgnc_id": 10680,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1942A>C",
          "hgvs_p": "p.Thr648Pro"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000651543.1",
          "gene_symbol": "ENSG00000286001",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.*675A>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " nuclear type 1,Hereditary cancer-predisposing syndrome,Mitochondrial complex II deficiency,Pheochromocytoma/paraganglioma syndrome 5,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:4",
      "phenotype_combined": "Pheochromocytoma/paraganglioma syndrome 5;Mitochondrial complex II deficiency, nuclear type 1|Hereditary cancer-predisposing syndrome|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}