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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-126346253-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=126346253&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "CENPW",
          "hgnc_id": 21488,
          "hgvs_c": "c.220A>G",
          "hgvs_p": "p.Arg74Gly",
          "inheritance_mode": "AR",
          "pathogenic_score": 3,
          "score": 3,
          "transcript": "NM_001286524.2",
          "verdict": "Uncertain_significance"
        },
        {
          "benign_score": 0,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "ENSG00000293110",
          "hgnc_id": null,
          "hgvs_c": "n.2418-39554T>C",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 3,
          "score": 3,
          "transcript": "ENST00000651326.1",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_score": 3,
      "allele_count_reference_population": 16,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.468,
      "alt": "G",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": 0,
      "chr": "6",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": "not specified",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "computational_prediction_selected": "Pathogenic",
      "computational_score_selected": 0.8275593519210815,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 88,
          "aa_ref": "R",
          "aa_start": 59,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 809,
          "cdna_start": 334,
          "cds_end": null,
          "cds_length": 267,
          "cds_start": 175,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "NM_001012507.4",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.175A>G",
          "hgvs_p": "p.Arg59Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000368328.5",
          "protein_coding": true,
          "protein_id": "NP_001012525.1",
          "strand": true,
          "transcript": "NM_001012507.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 88,
          "aa_ref": "R",
          "aa_start": 59,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 809,
          "cdna_start": 334,
          "cds_end": null,
          "cds_length": 267,
          "cds_start": 175,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000368328.5",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.175A>G",
          "hgvs_p": "p.Arg59Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001012507.4",
          "protein_coding": true,
          "protein_id": "ENSP00000357311.4",
          "strand": true,
          "transcript": "ENST00000368328.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 103,
          "aa_ref": "R",
          "aa_start": 74,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 854,
          "cdna_start": 379,
          "cds_end": null,
          "cds_length": 312,
          "cds_start": 220,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "NM_001286524.2",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.220A>G",
          "hgvs_p": "p.Arg74Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001273453.1",
          "strand": true,
          "transcript": "NM_001286524.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 103,
          "aa_ref": "R",
          "aa_start": 74,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 528,
          "cdna_start": 320,
          "cds_end": null,
          "cds_length": 312,
          "cds_start": 220,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000368325.5",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.220A>G",
          "hgvs_p": "p.Arg74Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000357308.1",
          "strand": true,
          "transcript": "ENST00000368325.5",
          "transcript_support_level": 3
        },
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 99,
          "aa_ref": "R",
          "aa_start": 74,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 529,
          "cdna_start": 333,
          "cds_end": null,
          "cds_length": 300,
          "cds_start": 220,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000930041.1",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.220A>G",
          "hgvs_p": "p.Arg74Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000600100.1",
          "strand": true,
          "transcript": "ENST00000930041.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "G",
          "aa_end": null,
          "aa_length": 84,
          "aa_ref": "R",
          "aa_start": 59,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 724,
          "cdna_start": 334,
          "cds_end": null,
          "cds_length": 255,
          "cds_start": 175,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000930040.1",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.175A>G",
          "hgvs_p": "p.Arg59Gly",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000600099.1",
          "strand": true,
          "transcript": "ENST00000930040.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "P",
          "aa_end": null,
          "aa_length": 67,
          "aa_ref": "P",
          "aa_start": 45,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 769,
          "cdna_start": 294,
          "cds_end": null,
          "cds_length": 204,
          "cds_start": 135,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "NM_001286525.2",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.135A>G",
          "hgvs_p": "p.Pro45Pro",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001273454.1",
          "strand": true,
          "transcript": "NM_001286525.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "P",
          "aa_end": null,
          "aa_length": 67,
          "aa_ref": "P",
          "aa_start": 45,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 443,
          "cdna_start": 235,
          "cds_end": null,
          "cds_length": 204,
          "cds_start": 135,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_count": 3,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "ENST00000368326.5",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.135A>G",
          "hgvs_p": "p.Pro45Pro",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000357309.1",
          "strand": true,
          "transcript": "ENST00000368326.5",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "P",
          "aa_end": null,
          "aa_length": 67,
          "aa_ref": "P",
          "aa_start": 45,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 363,
          "cdna_start": 294,
          "cds_end": null,
          "cds_length": 204,
          "cds_start": 135,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_count": 2,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "XM_017010845.2",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "c.135A>G",
          "hgvs_p": "p.Pro45Pro",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_016866334.1",
          "strand": true,
          "transcript": "XM_017010845.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3217,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 2,
          "exon_rank_end": null,
          "feature": "NR_104462.2",
          "gene_hgnc_id": 21488,
          "gene_symbol": "CENPW",
          "hgvs_c": "n.334A>G",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "NR_104462.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2558,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 7,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000651326.1",
          "gene_hgnc_id": null,
          "gene_symbol": "ENSG00000293110",
          "hgvs_c": "n.2418-39554T>C",
          "hgvs_p": null,
          "intron_rank": 6,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000651326.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1284,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000652383.1",
          "gene_hgnc_id": null,
          "gene_symbol": "ENSG00000293110",
          "hgvs_c": "n.631-35496T>C",
          "hgvs_p": null,
          "intron_rank": 3,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000652383.1",
          "transcript_support_level": null
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs143719119",
      "effect": "missense_variant",
      "frequency_reference_population": 0.000009944905,
      "gene_hgnc_id": 21488,
      "gene_symbol": "CENPW",
      "gnomad_exomes_ac": 12,
      "gnomad_exomes_af": 0.00000823826,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 4,
      "gnomad_genomes_af": 0.0000262733,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "not specified",
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 2.918,
      "pos": 126346253,
      "ref": "A",
      "revel_prediction": "Uncertain_significance",
      "revel_score": 0.323,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_001286524.2"
    }
  ]
}
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