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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-129503261-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=129503261&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 129503261,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000421865.3",
      "consequences": [
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 65,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.8528A>G",
          "hgvs_p": "p.Asn2843Ser",
          "transcript": "NM_000426.4",
          "protein_id": "NP_000417.3",
          "transcript_support_level": null,
          "aa_start": 2843,
          "aa_end": null,
          "aa_length": 3122,
          "cds_start": 8528,
          "cds_end": null,
          "cds_length": 9369,
          "cdna_start": 8636,
          "cdna_end": null,
          "cdna_length": 9696,
          "mane_select": "ENST00000421865.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 60,
          "exon_rank_end": null,
          "exon_count": 65,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.8528A>G",
          "hgvs_p": "p.Asn2843Ser",
          "transcript": "ENST00000421865.3",
          "protein_id": "ENSP00000400365.2",
          "transcript_support_level": 5,
          "aa_start": 2843,
          "aa_end": null,
          "aa_length": 3122,
          "cds_start": 8528,
          "cds_end": null,
          "cds_length": 9369,
          "cdna_start": 8636,
          "cdna_end": null,
          "cdna_length": 9696,
          "mane_select": "NM_000426.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 61,
          "exon_rank_end": null,
          "exon_count": 66,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.8792A>G",
          "hgvs_p": "p.Asn2931Ser",
          "transcript": "ENST00000618192.5",
          "protein_id": "ENSP00000480802.2",
          "transcript_support_level": 5,
          "aa_start": 2931,
          "aa_end": null,
          "aa_length": 3210,
          "cds_start": 8792,
          "cds_end": null,
          "cds_length": 9633,
          "cdna_start": 8900,
          "cdna_end": null,
          "cdna_length": 9960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.8516A>G",
          "hgvs_p": "p.Asn2839Ser",
          "transcript": "NM_001079823.2",
          "protein_id": "NP_001073291.2",
          "transcript_support_level": null,
          "aa_start": 2839,
          "aa_end": null,
          "aa_length": 3118,
          "cds_start": 8516,
          "cds_end": null,
          "cds_length": 9357,
          "cdna_start": 8624,
          "cdna_end": null,
          "cdna_length": 9684,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 59,
          "exon_rank_end": null,
          "exon_count": 64,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.8516A>G",
          "hgvs_p": "p.Asn2839Ser",
          "transcript": "ENST00000617695.5",
          "protein_id": "ENSP00000481744.2",
          "transcript_support_level": 5,
          "aa_start": 2839,
          "aa_end": null,
          "aa_length": 3118,
          "cds_start": 8516,
          "cds_end": null,
          "cds_length": 9357,
          "cdna_start": 8624,
          "cdna_end": null,
          "cdna_length": 9656,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.593A>G",
          "hgvs_p": "p.Asn198Ser",
          "transcript": "ENST00000494137.2",
          "protein_id": "ENSP00000510626.1",
          "transcript_support_level": 3,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": 993,
          "cdna_end": null,
          "cdna_length": 2015,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.593A>G",
          "hgvs_p": "p.Asn198Ser",
          "transcript": "ENST00000498257.6",
          "protein_id": "ENSP00000510533.1",
          "transcript_support_level": 5,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": 841,
          "cdna_end": null,
          "cdna_length": 1901,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "N",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "c.593A>G",
          "hgvs_p": "p.Asn198Ser",
          "transcript": "ENST00000688799.1",
          "protein_id": "ENSP00000508458.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 477,
          "cds_start": 593,
          "cds_end": null,
          "cds_length": 1434,
          "cdna_start": 690,
          "cdna_end": null,
          "cdna_length": 1750,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "n.1506A>G",
          "hgvs_p": null,
          "transcript": "ENST00000688198.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2528,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "n.1522A>G",
          "hgvs_p": null,
          "transcript": "ENST00000690858.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4443,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LAMA2",
          "gene_hgnc_id": 6482,
          "hgvs_c": "n.865A>G",
          "hgvs_p": null,
          "transcript": "ENST00000693461.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1888,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.807-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000657779.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3385,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1111-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000659721.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 1444,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1100-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000664071.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1445,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": 8,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.789-470T>C",
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          "transcript": "ENST00000665046.1",
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": 9,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1106-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000668058.1",
          "protein_id": null,
          "transcript_support_level": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 1578,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.471-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000810118.1",
          "protein_id": null,
          "transcript_support_level": null,
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          "cds_start": -4,
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          "cdna_length": 726,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000226149",
          "gene_hgnc_id": null,
          "hgvs_c": "n.573-470T>C",
          "hgvs_p": null,
          "transcript": "ENST00000810120.1",
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        },
        {
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          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "LOC102723409",
          "gene_hgnc_id": null,
          "hgvs_c": "n.14824-470T>C",
          "hgvs_p": null,
          "transcript": "XR_001743859.2",
          "protein_id": null,
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          "biotype": null,
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        },
        {
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "LOC102723409",
          "gene_hgnc_id": null,
          "hgvs_c": "n.12103-470T>C",
          "hgvs_p": null,
          "transcript": "XR_001743860.2",
          "protein_id": null,
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          "aa_start": null,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 14100,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "LOC102723409",
          "gene_hgnc_id": null,
          "hgvs_c": "n.12103-470T>C",
          "hgvs_p": null,
          "transcript": "XR_007059754.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": -4,
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          "cdna_start": null,
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      "frequency_reference_population": 0.0038595134,
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      "allele_count_reference_population": 6229,
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      "computational_prediction_selected": "Benign",
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      "splice_source_selected": "max_spliceai",
      "revel_score": 0.368,
      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.428,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "mitotip_score": null,
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      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
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          "criteria": [
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            "BP6_Very_Strong",
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          "verdict": "Benign",
          "transcript": "ENST00000421865.3",
          "gene_symbol": "LAMA2",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.8528A>G",
          "hgvs_p": "p.Asn2843Ser"
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        {
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          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000657779.1",
          "gene_symbol": "ENSG00000226149",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.807-470T>C",
          "hgvs_p": null
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "XR_001743859.2",
          "gene_symbol": "LOC102723409",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.14824-470T>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " autosomal recessive 23, limb-girdle,Congenital muscular dystrophy due to partial LAMA2 deficiency,LAMA2-related muscular dystrophy,Merosin deficient congenital muscular dystrophy,Muscular dystrophy,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:4 B:5",
      "phenotype_combined": "not specified|not provided|Congenital muscular dystrophy due to partial LAMA2 deficiency|Merosin deficient congenital muscular dystrophy|Muscular dystrophy, limb-girdle, autosomal recessive 23;Merosin deficient congenital muscular dystrophy|LAMA2-related muscular dystrophy",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}