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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-32183666-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=32183666&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 32183666,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001206929.2",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "NM_001136.5",
          "protein_id": "NP_001127.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000375076.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001136.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000375076.9",
          "protein_id": "ENSP00000364217.4",
          "transcript_support_level": 1,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001136.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375076.9"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000375069.7",
          "protein_id": "ENSP00000364210.4",
          "transcript_support_level": 1,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1263,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375069.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000438221.6",
          "protein_id": "ENSP00000387887.2",
          "transcript_support_level": 1,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000438221.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000375055.6",
          "protein_id": "ENSP00000364195.2",
          "transcript_support_level": 1,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 347,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1044,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375055.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.202G>A",
          "hgvs_p": "p.Gly68Ser",
          "transcript": "ENST00000375067.7",
          "protein_id": "ENSP00000364208.3",
          "transcript_support_level": 1,
          "aa_start": 68,
          "aa_end": null,
          "aa_length": 342,
          "cds_start": 202,
          "cds_end": null,
          "cds_length": 1029,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000375067.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "n.451G>A",
          "hgvs_p": null,
          "transcript": "ENST00000484849.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000484849.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "NM_001206929.2",
          "protein_id": "NP_001193858.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1263,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001206929.2"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.238G>A",
          "hgvs_p": "p.Gly80Ser",
          "transcript": "ENST00000851471.1",
          "protein_id": "ENSP00000521530.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851471.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Gly79Ser",
          "transcript": "ENST00000966081.1",
          "protein_id": "ENSP00000636140.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966081.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000851466.1",
          "protein_id": "ENSP00000521525.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851466.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.238G>A",
          "hgvs_p": "p.Gly80Ser",
          "transcript": "ENST00000966077.1",
          "protein_id": "ENSP00000636136.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966077.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Gly79Ser",
          "transcript": "ENST00000851480.1",
          "protein_id": "ENSP00000521539.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 414,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1245,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851480.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000966078.1",
          "protein_id": "ENSP00000636137.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 412,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1239,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966078.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.202G>A",
          "hgvs_p": "p.Gly68Ser",
          "transcript": "ENST00000851470.1",
          "protein_id": "ENSP00000521529.1",
          "transcript_support_level": null,
          "aa_start": 68,
          "aa_end": null,
          "aa_length": 406,
          "cds_start": 202,
          "cds_end": null,
          "cds_length": 1221,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851470.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000851475.1",
          "protein_id": "ENSP00000521534.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851475.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.238G>A",
          "hgvs_p": "p.Gly80Ser",
          "transcript": "ENST00000851463.1",
          "protein_id": "ENSP00000521522.1",
          "transcript_support_level": null,
          "aa_start": 80,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 238,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851463.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser",
          "transcript": "ENST00000966076.1",
          "protein_id": "ENSP00000636135.1",
          "transcript_support_level": null,
          "aa_start": 82,
          "aa_end": null,
          "aa_length": 402,
          "cds_start": 244,
          "cds_end": null,
          "cds_length": 1209,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966076.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Gly79Ser",
          "transcript": "ENST00000851468.1",
          "protein_id": "ENSP00000521527.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 401,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1206,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851468.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AGER",
          "gene_hgnc_id": 320,
          "hgvs_c": "c.232G>A",
          "hgvs_p": "p.Gly78Ser",
          "transcript": "ENST00000851478.1",
          "protein_id": "ENSP00000521537.1",
          "transcript_support_level": null,
          "aa_start": 78,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 232,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851478.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          "transcript": "ENST00000966079.1",
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        {
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          "consequences": [
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          "gene_symbol": "AGER",
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          "hgvs_c": "n.458G>A",
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          "transcript": "NR_038190.2",
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          "biotype": "pseudogene",
          "feature": "NR_038190.2"
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      ],
      "gene_symbol": "AGER",
      "gene_hgnc_id": 320,
      "dbsnp": "rs2070600",
      "frequency_reference_population": 0.056141622,
      "hom_count_reference_population": 3365,
      "allele_count_reference_population": 90557,
      "gnomad_exomes_af": 0.0579575,
      "gnomad_genomes_af": 0.0387192,
      "gnomad_exomes_ac": 84662,
      "gnomad_genomes_ac": 5895,
      "gnomad_exomes_homalt": 3131,
      "gnomad_genomes_homalt": 234,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.001693129539489746,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.7300000190734863,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.164,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3066,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.37,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.459,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.73,
      "spliceai_max_prediction": "Pathogenic",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -7,
      "acmg_classification": "Benign",
      "acmg_criteria": "PP3,BA1",
      "acmg_by_gene": [
        {
          "score": -7,
          "benign_score": 8,
          "pathogenic_score": 1,
          "criteria": [
            "PP3",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "NM_001206929.2",
          "gene_symbol": "AGER",
          "hgnc_id": 320,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.244G>A",
          "hgvs_p": "p.Gly82Ser"
        }
      ],
      "clinvar_disease": " severe early onset,COPD",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "COPD, severe early onset",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}