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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-52420435-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=52420435&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 52420435,
      "ref": "T",
      "alt": "C",
      "effect": "synonymous_variant",
      "transcript": "NM_018100.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "NM_018100.4",
          "protein_id": "NP_060570.2",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000371068.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_018100.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000371068.11",
          "protein_id": "ENSP00000360107.4",
          "transcript_support_level": 1,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_018100.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000371068.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "n.693T>C",
          "hgvs_p": null,
          "transcript": "ENST00000637340.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000637340.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-148T>C",
          "hgvs_p": null,
          "transcript": "ENST00000636702.1",
          "protein_id": "ENSP00000489623.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636702.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-161T>C",
          "hgvs_p": null,
          "transcript": "ENST00000636489.1",
          "protein_id": "ENSP00000489998.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 621,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1866,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636489.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000637353.1",
          "protein_id": "ENSP00000490441.1",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 641,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1926,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637353.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000635996.1",
          "protein_id": "ENSP00000490256.1",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 632,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1899,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000635996.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000637089.1",
          "protein_id": "ENSP00000489854.1",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 631,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1896,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637089.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000953671.1",
          "protein_id": "ENSP00000623730.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 593,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1782,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000953671.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000914231.1",
          "protein_id": "ENSP00000584290.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 589,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1770,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914231.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000636107.1",
          "protein_id": "ENSP00000489680.1",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 582,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1749,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000636107.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000914230.1",
          "protein_id": "ENSP00000584289.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000914230.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000637263.1",
          "protein_id": "ENSP00000489700.1",
          "transcript_support_level": 5,
          "aa_start": 9,
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          "aa_length": 552,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1659,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637263.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000636379.1",
          "protein_id": "ENSP00000490622.1",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 544,
          "cds_start": 25,
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          "cds_length": 1635,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000636379.1"
        },
        {
          "aa_ref": "L",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 1,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu",
          "transcript": "ENST00000953672.1",
          "protein_id": "ENSP00000623731.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 518,
          "cds_start": 25,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000953672.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-148T>C",
          "hgvs_p": null,
          "transcript": "ENST00000636702.1",
          "protein_id": "ENSP00000489623.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": null,
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          "cds_length": 1893,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000636702.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-161T>C",
          "hgvs_p": null,
          "transcript": "ENST00000636489.1",
          "protein_id": "ENSP00000489998.1",
          "transcript_support_level": 5,
          "aa_start": null,
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          "aa_length": 621,
          "cds_start": null,
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          "cds_length": 1866,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
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          "exon_count": 11,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-261-3511T>C",
          "hgvs_p": null,
          "transcript": "ENST00000635760.1",
          "protein_id": "ENSP00000489765.1",
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          "aa_length": 532,
          "cds_start": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000635760.1"
        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
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          ],
          "exon_rank": null,
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          "exon_count": 10,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.-261-3511T>C",
          "hgvs_p": null,
          "transcript": "ENST00000635984.1",
          "protein_id": "ENSP00000489921.1",
          "transcript_support_level": 5,
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          "aa_length": 474,
          "cds_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000635984.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "EFHC1",
          "gene_hgnc_id": 16406,
          "hgvs_c": "c.46-3511T>C",
          "hgvs_p": null,
          "transcript": "ENST00000637315.1",
          "protein_id": "ENSP00000489708.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": null,
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          "cds_length": 445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000637315.1"
        },
        {
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      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "alphamissense_score": null,
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      "bayesdelnoaf_score": -0.61,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.419,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
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      "acmg_score": -17,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BP7,BS2",
      "acmg_by_gene": [
        {
          "score": -17,
          "benign_score": 17,
          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BP7",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_018100.4",
          "gene_symbol": "EFHC1",
          "hgnc_id": 16406,
          "effects": [
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          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.25T>C",
          "hgvs_p": "p.Leu9Leu"
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        {
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          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000729269.1",
          "gene_symbol": "ENSG00000295329",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.547A>G",
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        },
        {
          "score": -16,
          "benign_score": 16,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "XR_007059611.1",
          "gene_symbol": "LOC124901331",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.547A>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": " 1, juvenile, susceptibility to,Absence seizure,EFHC1-related disorder,Juvenile myoclonic epilepsy,Myoclonic epilepsy,not provided,not specified",
      "clinvar_classification": "Benign/Likely benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "LB:2 B:3",
      "phenotype_combined": "not specified|Juvenile myoclonic epilepsy|EFHC1-related disorder|not provided|Absence seizure;Myoclonic epilepsy, juvenile, susceptibility to, 1",
      "pathogenicity_classification_combined": "Benign/Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}