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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-5260703-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=5260703&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 5260703,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000330636.9",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "NM_020408.6",
          "protein_id": "NP_065141.3",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 91,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 276,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 1497,
          "mane_select": "ENST00000330636.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "ENST00000330636.9",
          "protein_id": "ENSP00000418787.1",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 91,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 276,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 1497,
          "mane_select": "NM_020408.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "ENST00000480566.5",
          "protein_id": "ENSP00000419928.1",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 70,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 213,
          "cdna_start": 40,
          "cdna_end": null,
          "cdna_length": 941,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "ENST00000468929.5",
          "protein_id": "ENSP00000418321.1",
          "transcript_support_level": 1,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 40,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 123,
          "cdna_start": 40,
          "cdna_end": null,
          "cdna_length": 1168,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "NM_001164840.3",
          "protein_id": "NP_001158312.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 130,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 393,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 805,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "ENST00000500576.4",
          "protein_id": "ENSP00000443900.3",
          "transcript_support_level": 2,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 130,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 393,
          "cdna_start": 35,
          "cdna_end": null,
          "cdna_length": 592,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "NM_001318783.1",
          "protein_id": "NP_001305712.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 115,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 348,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 764,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "NM_001164841.3",
          "protein_id": "NP_001158313.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 96,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 291,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 1622,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "ENST00000464010.5",
          "protein_id": "ENSP00000420026.1",
          "transcript_support_level": 3,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 96,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 291,
          "cdna_start": 31,
          "cdna_end": null,
          "cdna_length": 1401,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "NM_001318782.1",
          "protein_id": "NP_001305711.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 70,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 213,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 1149,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "XM_017011084.3",
          "protein_id": "XP_016866573.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 129,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 390,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 3435,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala",
          "transcript": "XM_017011083.3",
          "protein_id": "XP_016866572.1",
          "transcript_support_level": null,
          "aa_start": 11,
          "aa_end": null,
          "aa_length": 97,
          "cds_start": 31,
          "cds_end": null,
          "cds_length": 294,
          "cdna_start": 248,
          "cdna_end": null,
          "cdna_length": 876,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "n.19T>G",
          "hgvs_p": null,
          "transcript": "ENST00000455814.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 450,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "n.248T>G",
          "hgvs_p": null,
          "transcript": "NR_104417.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1844,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "n.248T>G",
          "hgvs_p": null,
          "transcript": "NR_104418.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1376,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LYRM4",
          "gene_hgnc_id": 21365,
          "hgvs_c": "n.248T>G",
          "hgvs_p": null,
          "transcript": "NR_134856.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 856,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FARS2",
          "gene_hgnc_id": 21062,
          "hgvs_c": "c.-22+10750A>C",
          "hgvs_p": null,
          "transcript": "XM_047418086.1",
          "protein_id": "XP_047274042.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 451,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1356,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1551,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "LYRM4",
      "gene_hgnc_id": 21365,
      "dbsnp": "rs2224391",
      "frequency_reference_population": 0.27694646,
      "hom_count_reference_population": 71365,
      "allele_count_reference_population": 430187,
      "gnomad_exomes_af": 0.262923,
      "gnomad_genomes_af": 0.406373,
      "gnomad_exomes_ac": 368480,
      "gnomad_genomes_ac": 61707,
      "gnomad_exomes_homalt": 54603,
      "gnomad_genomes_homalt": 16762,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.000011671280844893772,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.093,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0928,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.58,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.361,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000330636.9",
          "gene_symbol": "LYRM4",
          "hgnc_id": 21365,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown,AR",
          "hgvs_c": "c.31T>G",
          "hgvs_p": "p.Ser11Ala"
        },
        {
          "score": -20,
          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "XM_047418086.1",
          "gene_symbol": "FARS2",
          "hgnc_id": 21062,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-22+10750A>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Combined oxidative phosphorylation deficiency 19,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:3",
      "phenotype_combined": "not specified|not provided|Combined oxidative phosphorylation deficiency 19",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}