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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 6-87514471-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=6&pos=87514471&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "6",
      "pos": 87514471,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001350505.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1679G>T",
          "hgvs_p": "p.Arg560Leu",
          "transcript": "NM_020320.5",
          "protein_id": "NP_064716.2",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000369536.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020320.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1679G>T",
          "hgvs_p": "p.Arg560Leu",
          "transcript": "ENST00000369536.10",
          "protein_id": "ENSP00000358549.5",
          "transcript_support_level": 1,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 578,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1737,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020320.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000369536.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1769G>T",
          "hgvs_p": "p.Arg590Leu",
          "transcript": "ENST00000687437.1",
          "protein_id": "ENSP00000508968.1",
          "transcript_support_level": null,
          "aa_start": 590,
          "aa_end": null,
          "aa_length": 608,
          "cds_start": 1769,
          "cds_end": null,
          "cds_length": 1827,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000687437.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1679G>T",
          "hgvs_p": "p.Arg560Leu",
          "transcript": "NM_001350505.2",
          "protein_id": "NP_001337434.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 588,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1767,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350505.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1679G>T",
          "hgvs_p": "p.Arg560Leu",
          "transcript": "ENST00000691725.1",
          "protein_id": "ENSP00000509453.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 588,
          "cds_start": 1679,
          "cds_end": null,
          "cds_length": 1767,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000691725.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1694G>T",
          "hgvs_p": "p.Arg565Leu",
          "transcript": "ENST00000879834.1",
          "protein_id": "ENSP00000549893.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879834.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1268G>T",
          "hgvs_p": "p.Arg423Leu",
          "transcript": "ENST00000451155.2",
          "protein_id": "ENSP00000389656.2",
          "transcript_support_level": 3,
          "aa_start": 423,
          "aa_end": null,
          "aa_length": 441,
          "cds_start": 1268,
          "cds_end": null,
          "cds_length": 1326,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000451155.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350506.2",
          "protein_id": "NP_001337435.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 1154,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001350506.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "ENST00000693431.1",
          "protein_id": "ENSP00000509147.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 1154,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000693431.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001318785.2",
          "protein_id": "NP_001305714.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1154,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318785.2"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350507.2",
          "protein_id": "NP_001337436.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1154,
          "cds_end": null,
          "cds_length": 1212,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350508.2",
          "protein_id": "NP_001337437.1",
          "transcript_support_level": null,
          "aa_start": 385,
          "aa_end": null,
          "aa_length": 403,
          "cds_start": 1154,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
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          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350509.2",
          "protein_id": "NP_001337438.1",
          "transcript_support_level": null,
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          "aa_length": 403,
          "cds_start": 1154,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350510.2",
          "protein_id": "NP_001337439.1",
          "transcript_support_level": null,
          "aa_start": 385,
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          "cds_start": 1154,
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          "cdna_start": null,
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        {
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          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "NM_001350511.2",
          "protein_id": "NP_001337440.1",
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          "aa_length": 403,
          "cds_start": 1154,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001350511.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "ENST00000685408.1",
          "protein_id": "ENSP00000509026.1",
          "transcript_support_level": null,
          "aa_start": 385,
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          "cds_start": 1154,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          "consequences": [
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          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
          "hgvs_p": "p.Arg385Leu",
          "transcript": "ENST00000689174.1",
          "protein_id": "ENSP00000510542.1",
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        {
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          "exon_count": 20,
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          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1154G>T",
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          "transcript": "ENST00000692684.1",
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          "cds_start": 1154,
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        },
        {
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          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RARS2",
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          "hgvs_c": "c.1154G>T",
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          "protein_id": "ENSP00000509195.1",
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          "biotype": "protein_coding",
          "feature": "ENST00000693327.1"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "c.1097G>T",
          "hgvs_p": "p.Arg366Leu",
          "transcript": "ENST00000687729.1",
          "protein_id": "ENSP00000508582.1",
          "transcript_support_level": null,
          "aa_start": 366,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": 1097,
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          "cdna_start": null,
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          "cdna_length": null,
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          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000691238.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "n.*595G>T",
          "hgvs_p": null,
          "transcript": "ENST00000691815.1",
          "protein_id": "ENSP00000509579.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000691815.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "n.*595G>T",
          "hgvs_p": null,
          "transcript": "ENST00000692270.1",
          "protein_id": "ENSP00000510055.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000692270.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "n.*666G>T",
          "hgvs_p": null,
          "transcript": "ENST00000692843.1",
          "protein_id": "ENSP00000509592.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000692843.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RARS2",
          "gene_hgnc_id": 21406,
          "hgvs_c": "n.*595G>T",
          "hgvs_p": null,
          "transcript": "ENST00000693605.1",
          "protein_id": "ENSP00000510050.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000693605.1"
        }
      ],
      "gene_symbol": "RARS2",
      "gene_hgnc_id": 21406,
      "dbsnp": "rs756502974",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": null,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8913782835006714,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.629,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.373,
      "alphamissense_prediction": "Uncertain_significance",
      "bayesdelnoaf_score": 0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.297,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PM5,PP3_Moderate",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PM5",
            "PP3_Moderate"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001350505.2",
          "gene_symbol": "RARS2",
          "hgnc_id": 21406,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1679G>T",
          "hgvs_p": "p.Arg560Leu"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}