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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 7-66089678-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=7&pos=66089678&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "7",
      "pos": 66089678,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000304874.14",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.1045G>A",
          "hgvs_p": "p.Val349Ile",
          "transcript": "NM_000048.4",
          "protein_id": "NP_000039.2",
          "transcript_support_level": null,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1045,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1126,
          "cdna_end": null,
          "cdna_length": 2140,
          "mane_select": "ENST00000304874.14",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.1045G>A",
          "hgvs_p": "p.Val349Ile",
          "transcript": "ENST00000304874.14",
          "protein_id": "ENSP00000307188.9",
          "transcript_support_level": 1,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1045,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1126,
          "cdna_end": null,
          "cdna_length": 2140,
          "mane_select": "NM_000048.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.1045G>A",
          "hgvs_p": "p.Val349Ile",
          "transcript": "ENST00000395332.8",
          "protein_id": "ENSP00000378741.3",
          "transcript_support_level": 1,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1045,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1238,
          "cdna_end": null,
          "cdna_length": 1608,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000249319",
          "gene_hgnc_id": null,
          "hgvs_c": "c.358G>A",
          "hgvs_p": "p.Val120Ile",
          "transcript": "ENST00000450043.2",
          "protein_id": "ENSP00000396527.2",
          "transcript_support_level": 5,
          "aa_start": 120,
          "aa_end": null,
          "aa_length": 306,
          "cds_start": 358,
          "cds_end": null,
          "cds_length": 922,
          "cdna_start": 358,
          "cdna_end": null,
          "cdna_length": 922,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.1045G>A",
          "hgvs_p": "p.Val349Ile",
          "transcript": "NM_001024943.2",
          "protein_id": "NP_001020114.1",
          "transcript_support_level": null,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1045,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1308,
          "cdna_end": null,
          "cdna_length": 2322,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.985G>A",
          "hgvs_p": "p.Val329Ile",
          "transcript": "NM_001024944.2",
          "protein_id": "NP_001020115.1",
          "transcript_support_level": null,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 985,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": 1248,
          "cdna_end": null,
          "cdna_length": 2262,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.985G>A",
          "hgvs_p": "p.Val329Ile",
          "transcript": "ENST00000395331.4",
          "protein_id": "ENSP00000378740.3",
          "transcript_support_level": 5,
          "aa_start": 329,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 985,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": 1123,
          "cdna_end": null,
          "cdna_length": 1478,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.967G>A",
          "hgvs_p": "p.Val323Ile",
          "transcript": "NM_001024946.2",
          "protein_id": "NP_001020117.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": 1230,
          "cdna_end": null,
          "cdna_length": 2244,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.967G>A",
          "hgvs_p": "p.Val323Ile",
          "transcript": "ENST00000380839.9",
          "protein_id": "ENSP00000370219.4",
          "transcript_support_level": 5,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": 1202,
          "cdna_end": null,
          "cdna_length": 1974,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.967G>A",
          "hgvs_p": "p.Val323Ile",
          "transcript": "ENST00000673518.1",
          "protein_id": "ENSP00000499889.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 438,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1317,
          "cdna_start": 1045,
          "cdna_end": null,
          "cdna_length": 1436,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.850G>A",
          "hgvs_p": "p.Val284Ile",
          "transcript": "ENST00000362000.10",
          "protein_id": "ENSP00000354710.6",
          "transcript_support_level": 2,
          "aa_start": 284,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 850,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 995,
          "cdna_end": null,
          "cdna_length": 1388,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.967G>A",
          "hgvs_p": "p.Val323Ile",
          "transcript": "ENST00000671817.1",
          "protein_id": "ENSP00000500462.1",
          "transcript_support_level": null,
          "aa_start": 323,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 967,
          "cds_end": null,
          "cds_length": 1042,
          "cdna_start": 1104,
          "cdna_end": null,
          "cdna_length": 1179,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.164G>A",
          "hgvs_p": null,
          "transcript": "ENST00000464970.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 640,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.526G>A",
          "hgvs_p": null,
          "transcript": "ENST00000493708.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 885,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.*344G>A",
          "hgvs_p": null,
          "transcript": "ENST00000672498.1",
          "protein_id": "ENSP00000500227.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3323,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.1804G>A",
          "hgvs_p": null,
          "transcript": "ENST00000672586.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2172,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.2069G>A",
          "hgvs_p": null,
          "transcript": "ENST00000672676.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2441,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.857G>A",
          "hgvs_p": null,
          "transcript": "ENST00000673149.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
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          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 1221,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.3162G>A",
          "hgvs_p": null,
          "transcript": "ENST00000673350.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3520,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "n.*344G>A",
          "hgvs_p": null,
          "transcript": "ENST00000672498.1",
          "protein_id": "ENSP00000500227.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 3323,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ASL",
          "gene_hgnc_id": 746,
          "hgvs_c": "c.147+343G>A",
          "hgvs_p": null,
          "transcript": "ENST00000488343.2",
          "protein_id": "ENSP00000500864.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 51,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 156,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 198,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "ASL",
      "gene_hgnc_id": 746,
      "dbsnp": "rs372774556",
      "frequency_reference_population": 0.000019214105,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 31,
      "gnomad_exomes_af": 0.0000164243,
      "gnomad_genomes_af": 0.0000460066,
      "gnomad_exomes_ac": 24,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.384130597114563,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.426,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1009,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 4.112,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP2,BP4",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 1,
          "pathogenic_score": 1,
          "criteria": [
            "PP2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000304874.14",
          "gene_symbol": "ASL",
          "hgnc_id": 746,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1045G>A",
          "hgvs_p": "p.Val349Ile"
        },
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000450043.2",
          "gene_symbol": "ENSG00000249319",
          "hgnc_id": null,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.358G>A",
          "hgvs_p": "p.Val120Ile"
        }
      ],
      "clinvar_disease": "Argininosuccinate lyase deficiency,Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:3",
      "phenotype_combined": "Argininosuccinate lyase deficiency|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}