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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 8-144511458-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=8&pos=144511458&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "8",
      "pos": 144511458,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "NM_001413019.1",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3600G>A",
          "hgvs_p": "p.Thr1200Thr",
          "transcript": "NM_004260.4",
          "protein_id": "NP_004251.4",
          "transcript_support_level": null,
          "aa_start": 1200,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3600,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000617875.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004260.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3600G>A",
          "hgvs_p": "p.Thr1200Thr",
          "transcript": "ENST00000617875.6",
          "protein_id": "ENSP00000482313.2",
          "transcript_support_level": 1,
          "aa_start": 1200,
          "aa_end": null,
          "aa_length": 1208,
          "cds_start": 3600,
          "cds_end": null,
          "cds_length": 3627,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004260.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000617875.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2529G>A",
          "hgvs_p": "p.Thr843Thr",
          "transcript": "ENST00000621189.4",
          "protein_id": "ENSP00000483145.1",
          "transcript_support_level": 1,
          "aa_start": 843,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2529,
          "cds_end": null,
          "cds_length": 2556,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000621189.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3675G>A",
          "hgvs_p": "p.Thr1225Thr",
          "transcript": "NM_001413019.1",
          "protein_id": "NP_001399948.1",
          "transcript_support_level": null,
          "aa_start": 1225,
          "aa_end": null,
          "aa_length": 1233,
          "cds_start": 3675,
          "cds_end": null,
          "cds_length": 3702,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413019.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3609G>A",
          "hgvs_p": "p.Thr1203Thr",
          "transcript": "NM_001413036.1",
          "protein_id": "NP_001399965.1",
          "transcript_support_level": null,
          "aa_start": 1203,
          "aa_end": null,
          "aa_length": 1211,
          "cds_start": 3609,
          "cds_end": null,
          "cds_length": 3636,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413036.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3570G>A",
          "hgvs_p": "p.Thr1190Thr",
          "transcript": "NM_001413039.1",
          "protein_id": "NP_001399968.1",
          "transcript_support_level": null,
          "aa_start": 1190,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 3570,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413039.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3507G>A",
          "hgvs_p": "p.Thr1169Thr",
          "transcript": "ENST00000971710.1",
          "protein_id": "ENSP00000641769.1",
          "transcript_support_level": null,
          "aa_start": 1169,
          "aa_end": null,
          "aa_length": 1177,
          "cds_start": 3507,
          "cds_end": null,
          "cds_length": 3534,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000971710.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3504G>A",
          "hgvs_p": "p.Thr1168Thr",
          "transcript": "NM_001413020.1",
          "protein_id": "NP_001399949.1",
          "transcript_support_level": null,
          "aa_start": 1168,
          "aa_end": null,
          "aa_length": 1176,
          "cds_start": 3504,
          "cds_end": null,
          "cds_length": 3531,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413020.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3471G>A",
          "hgvs_p": "p.Thr1157Thr",
          "transcript": "NM_001413025.1",
          "protein_id": "NP_001399954.1",
          "transcript_support_level": null,
          "aa_start": 1157,
          "aa_end": null,
          "aa_length": 1165,
          "cds_start": 3471,
          "cds_end": null,
          "cds_length": 3498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413025.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3468G>A",
          "hgvs_p": "p.Thr1156Thr",
          "transcript": "NM_001413033.1",
          "protein_id": "NP_001399962.1",
          "transcript_support_level": null,
          "aa_start": 1156,
          "aa_end": null,
          "aa_length": 1164,
          "cds_start": 3468,
          "cds_end": null,
          "cds_length": 3495,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413033.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3468G>A",
          "hgvs_p": "p.Thr1156Thr",
          "transcript": "ENST00000887977.1",
          "protein_id": "ENSP00000558036.1",
          "transcript_support_level": null,
          "aa_start": 1156,
          "aa_end": null,
          "aa_length": 1164,
          "cds_start": 3468,
          "cds_end": null,
          "cds_length": 3495,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000887977.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3438G>A",
          "hgvs_p": "p.Thr1146Thr",
          "transcript": "ENST00000922086.1",
          "protein_id": "ENSP00000592145.1",
          "transcript_support_level": null,
          "aa_start": 1146,
          "aa_end": null,
          "aa_length": 1154,
          "cds_start": 3438,
          "cds_end": null,
          "cds_length": 3465,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000922086.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3402G>A",
          "hgvs_p": "p.Thr1134Thr",
          "transcript": "NM_001413018.1",
          "protein_id": "NP_001399947.1",
          "transcript_support_level": null,
          "aa_start": 1134,
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          "aa_length": 1142,
          "cds_start": 3402,
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          "cds_length": 3429,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413018.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3306G>A",
          "hgvs_p": "p.Thr1102Thr",
          "transcript": "NM_001413017.1",
          "protein_id": "NP_001399946.1",
          "transcript_support_level": null,
          "aa_start": 1102,
          "aa_end": null,
          "aa_length": 1110,
          "cds_start": 3306,
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          "biotype": "protein_coding",
          "feature": "NM_001413017.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3249G>A",
          "hgvs_p": "p.Thr1083Thr",
          "transcript": "NM_001413029.1",
          "protein_id": "NP_001399958.1",
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          "aa_end": null,
          "aa_length": 1091,
          "cds_start": 3249,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413029.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 21,
          "exon_rank_end": null,
          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.3156G>A",
          "hgvs_p": "p.Thr1052Thr",
          "transcript": "ENST00000922087.1",
          "protein_id": "ENSP00000592146.1",
          "transcript_support_level": null,
          "aa_start": 1052,
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          "aa_length": 1060,
          "cds_start": 3156,
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          "cds_length": 3183,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000922087.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2604G>A",
          "hgvs_p": "p.Thr868Thr",
          "transcript": "NM_001413023.1",
          "protein_id": "NP_001399952.1",
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          "biotype": "protein_coding",
          "feature": "NM_001413023.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
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          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2538G>A",
          "hgvs_p": "p.Thr846Thr",
          "transcript": "NM_001413041.1",
          "protein_id": "NP_001399970.1",
          "transcript_support_level": null,
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          "cds_length": 2565,
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          "biotype": "protein_coding",
          "feature": "NM_001413041.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
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          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2529G>A",
          "hgvs_p": "p.Thr843Thr",
          "transcript": "NM_001413021.1",
          "protein_id": "NP_001399950.1",
          "transcript_support_level": null,
          "aa_start": 843,
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          "cds_start": 2529,
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          "cds_length": 2556,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001413021.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL4",
          "gene_hgnc_id": 9949,
          "hgvs_c": "c.2529G>A",
          "hgvs_p": "p.Thr843Thr",
          "transcript": "NM_001413022.1",
          "protein_id": "NP_001399951.1",
          "transcript_support_level": null,
          "aa_start": 843,
          "aa_end": null,
          "aa_length": 851,
          "cds_start": 2529,
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          "transcript": "XM_011516806.3",
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        {
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          "transcript": "NR_130120.2",
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          "biotype": "pseudogene",
          "feature": "NR_130120.2"
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      ],
      "gene_symbol": "RECQL4",
      "gene_hgnc_id": 9949,
      "dbsnp": "rs35225938",
      "frequency_reference_population": 0.00015193308,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 245,
      "gnomad_exomes_af": 0.000105466,
      "gnomad_genomes_af": 0.000597262,
      "gnomad_exomes_ac": 154,
      "gnomad_genomes_ac": 91,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.6700000166893005,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.67,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -2.402,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -6,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6,BP7",
      "acmg_by_gene": [
        {
          "score": -6,
          "benign_score": 6,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001413019.1",
          "gene_symbol": "RECQL4",
          "hgnc_id": 9949,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.3675G>A",
          "hgvs_p": "p.Thr1225Thr"
        },
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_138431.3",
          "gene_symbol": "SLC33A2",
          "hgnc_id": 25157,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*294C>T",
          "hgvs_p": null
        },
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000301327.5",
          "gene_symbol": "MFSD3",
          "hgnc_id": 25157,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*294C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Baller-Gerold syndrome,Hereditary cancer-predisposing syndrome,RECQL4-related disorder,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 LB:2",
      "phenotype_combined": "not provided|Baller-Gerold syndrome|RECQL4-related disorder|Hereditary cancer-predisposing syndrome",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}